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Mouse anti-Human CD45 (LCA) Monoclonal Antibody

Mouse Anti-Human CD45/LCA-PE/CY7

Reactivity
Human
Applications
Flow Cytometry
Synonyms
CD45 (LCA); Monoclonal Antibody; Mouse Anti-Human CD45/LCA-PE/CY7; Mouse Anti-Human CD45/LCA - Phycoerythrin-Labeled/CY7; Mouse Anti-Human CD45 (LCA); anti-CD45 (LCA) antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
Mouse IgG2a
Clone Number
F10-89-4
Specificity
Human/Horse CD45; Mr 180-220 kDa
Form/Format
R - phycoerythrin - Cyanine 7 (R - PE - CY7) Conjugate
Applicable Applications for anti-CD45 (LCA) antibody
Flow Cytometry
Application Notes
Flow Cytometry:

Purified (UNLB) antibody: < = 1ug/10^6 cells
FITC,BIOT,PE,PE/TXRD, APC, SPRD,CY5,PE/CY5.5M ABD PE/CY7 conjugates: 10ul/10^6 cells

For flow cytometry, the suggested use of these reagents is in a final volume of 100ul

Other Application:
Since applications vary, you should determine the optimum working dilution for the product that is appropriate for your specific need.
Immunogen
Purified T cells from human lymph nodes
Workshop
IV N819
Characterization
To insure lot-to-lot consistency, each batch of product is tested by flow cytometry to conform to characteristics of a standard reference reagent.
Preparation and Storage
The purified (UNLB) antibody is supplied as 0.1 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.0. No preservatives or amine-containing buffer salts added. Store at 2- 8 degree C.
The fluorescein (FITC) conjugate is supplied as 100 tests in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The biotin (BIOT) conjugate is supplied as 100 tests in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The R-phycoerythrin (R-PE) and Allophycocyanin conjugates are supplied as 100 tests in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The Spectral Red (SPRD), R-phycoerythrin-Cyanine 5.5 (R-PE-CY 5.5), and R-phycoerythrin- Cyanine 7 (R-PE-CY 7) conjugates are supplied as 100 tests in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The Cyanine 5 (CY 5) conjugate is supplied as 100 tests in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
Protect conjugated forms from light. Reagents are stable for the period shown on the label if stored as directed.
Related Product Information for anti-CD45 (LCA) antibody
CD45, also known as the leukocyte common antigen (LCA), exists as four different isoforms (180, 190, 205, 220 kDa) that are generated by alternative splicing of three exons. LCA is found on all cells of hematopoietic origin, except erythrocytes and platelets. CD45 expression is required for T cell and B cell antigen receptor-mediated activation.1-4
References
1. McMichael, A.K., P.C.L. Beverly, S. Cobbold, M.J. Crumpton, W. Gilks, F.M. Gotch, N. Hogg, M. Horton, N. Ling, I.C.M. MacLennan, D.Y. Mason, C. Milstein, D. Spiegelhalter, and H. Waldmann, eds. 1987. Leukocyte Typing III: White Cell Differentiation Antigens, Oxford University Press, Oxford.
2. Barclay, A.N., M.H. Brown, S.K.A. Law, A.J. McKnight, M.G. Tomlinson, and P.A. van der Merwe, eds. 1997. The Leukocyte Antigens Facts Book, 2nd Edition, CD45 Section, Academic Press, New York, p. 244.
3. Dalchau, R., J. Kirkley, and J.W. Fabre. Eur. J. Immunol. 10:737.
4. Thomas, M.L. 1989. Annu. Rev. Immunol. 7:339.
5. Trowbridge, I.S., and M.L. Thomas. 1994. Annu. Rev. Immunol. 12:85.

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Product Notes

The CD45 (LCA) (Catalog #AAA674265) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Anti-Human CD45/LCA-PE/CY7 reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD45 (LCA) can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry. Flow Cytometry: Purified (UNLB) antibody: < = 1ug/10^6 cells FITC,BIOT,PE,PE/TXRD, APC, SPRD,CY5,PE/CY5.5M ABD PE/CY7 conjugates: 10ul/10^6 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100ul Other Application: Since applications vary, you should determine the optimum working dilution for the product that is appropriate for your specific need. Researchers should empirically determine the suitability of the CD45 (LCA) for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD45 (LCA), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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