Flow Cytometry (FC/FACS)
(Flow cytometry analysis (surface staining) of murine splenocytes with anti-CD45 antibody EM-05 PE.)
Rat anti-Mouse CD45 Monoclonal Antibody | anti-CD45 antibody
Anti-Ms CD45 PE
Gene Names
Ptprc; loc; B220; Cd45; L-CA; Ly-5; T200; CD45R; Lyt-4
Reactivity
Mouse
Applications
Flow Cytometry, Functional Assay
Purity
The purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Synonyms
CD45; Monoclonal Antibody; Anti-Ms CD45 PE; protein tyrosine phosphatase; receptor type; C; LCA; Receptor-type tyrosine-protein phosphatase C; Leukocyte common antigen; L-CA; T200; LY5; GP180; anti-CD45 antibody
Host
Rat
Reactivity
Mouse
Clonality
Monoclonal
Isotype
IgG
Clone Number
EM-05
Specificity
The rat monoclonal antibody EM-05 reacts with an extracellular epitope of murine CD45 antigen (Leukocyte Common Antigen), a single chain type I transmembrane protein expressed at high level on cells of hematopoietic origin, except erythrocytes and platelets.
Purity/Purification
The purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Form/Format
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Concentration
0.5 mg/ml (varies by lot)
Sequence Length
879
Applicable Applications for anti-CD45 antibody
Flow Cytometry (FC/FACS)
Application Notes
FC/FACS: QC tested
Immunogen
Murine peripheral blood leukocytes
Conjugation
PE
Preparation and Storage
Store in the dark at 2-8 degree C. Do not freeze. Avoid prolonged exposure to light.
Flow Cytometry (FC/FACS)
(Flow cytometry analysis (surface staining) of murine splenocytes with anti-CD45 antibody EM-05 PE.)
Flow Cytometry (FC/FACS)
(Flow cytometry analysis (surface staining) of murine splenocytes with anti-CD45 antibody EM-05 PE.)
Related Product Information for anti-CD45 antibody
CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing; these isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. These isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. Besides the role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
2 days pregnant adult female oviduct cDNA, RIKEN full-length enriched library, clone:E230015G23 product:protein tyrosine phosphatase, receptor type, C, full insert sequence
NCBI Official Synonym Full Names
protein tyrosine phosphatase, receptor type, C
NCBI Official Symbol
Ptprc
NCBI Official Synonym Symbols
loc; B220; Cd45; L-CA; Ly-5; T200; CD45R; Lyt-4
NCBI Protein Information
receptor-type tyrosine-protein phosphatase C
Research Articles on CD45
Similar Products
Product Notes
The CD45 (Catalog #AAA1800588) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The Anti-Ms CD45 PE reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CD45 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS). FC/FACS: QC tested. Researchers should empirically determine the suitability of the CD45 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD45, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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