Flow Cytometry (FC/FACS)
(C57BL/6 murine splenocytes are stained with Anti-Mouse CD45 Monoclonal Antibody(PE/Cyanine5.5 Conjugated)(filled gray histogram). Unstained splenocytes (empty black histogram) are used as control.)
Rat anti-Mouse CD45 Monoclonal Antibody | anti-CD45 antibody
Anti-Mouse CD45 Monoclonal Antibody (PE/Cyanine5.5 Conjugated) [30-F11]
Gene Names
Ptprc; loc; B220; Cd45; L-CA; Ly-5; T200; CD45R; Lyt-4
Reactivity
Mouse
Applications
Flow Cytometry, Functional Assay
Synonyms
CD45; Monoclonal Antibody; Anti-Mouse CD45 Monoclonal Antibody (PE/Cyanine5.5 Conjugated) [30-F11]; Receptor-type tyrosine-protein phosphatase C; Ptprc; L-CA; Ly-5; T200; anti-CD45 antibody
Host
Rat
Reactivity
Mouse
Clonality
Monoclonal
Isotype
IgG2b, kappa
Clone Number
30-F11
Form/Format
PBS with 0.05% Proclin300, 1% BSA
Concentration
0.5 mg/mL (varies by lot)
Sequence Length
1293
Applicable Applications for anti-CD45 antibody
Flow Cytometry (FC/FACS)
Application Notes
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is <= 1 ug per 106 cells in 100 ul volume or 100 ul of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Conjugation
PE/Cyanine5.5
Preparation and Storage
Store at 2-8 degree C and protect from prolonged exposure to light. Do not freeze.
Flow Cytometry (FC/FACS)
(C57BL/6 murine splenocytes are stained with Anti-Mouse CD45 Monoclonal Antibody(PE/Cyanine5.5 Conjugated)(filled gray histogram). Unstained splenocytes (empty black histogram) are used as control.)
Flow Cytometry (FC/FACS)
(C57BL/6 murine splenocytes are stained with Anti-Mouse CD45 Monoclonal Antibody(PE/Cyanine5.5 Conjugated)(filled gray histogram). Unstained splenocytes (empty black histogram) are used as control.)
Related Product Information for anti-CD45 antibody
CD45 is a 180-240 kD glycoprotein also known as the leukocyte common antigen (LCA), T200, or Ly-5. It is a member of the protein tyrosine phosphatase (PTP) family, expressed on all hematopoietic cells except mature erythrocytes and platelets. There are different isoforms of CD45 that arise from alternative splicing of exons 4, 5, and 6, which encode A, B, and C determinants, respectively. CD45 plays a key role in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation state of the cell as well as cell type. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
Receptor-type tyrosine-protein phosphatase C
NCBI Official Synonym Full Names
protein tyrosine phosphatase, receptor type, C
NCBI Official Symbol
Ptprc
NCBI Official Synonym Symbols
loc; B220; Cd45; L-CA; Ly-5; T200; CD45R; Lyt-4
NCBI Protein Information
receptor-type tyrosine-protein phosphatase C
UniProt Protein Name
Receptor-type tyrosine-protein phosphatase C
UniProt Gene Name
Ptprc
UniProt Synonym Gene Names
Ly-5
UniProt Entry Name
PTPRC_MOUSE
Research Articles on CD45
Similar Products
Product Notes
The CD45 ptprc (Catalog #AAA2558991) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The Anti-Mouse CD45 Monoclonal Antibody (PE/Cyanine5.5 Conjugated) [30-F11] reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CD45 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS). Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is <= 1 ug per 106 cells in 100 ul volume or 100 ul of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. Researchers should empirically determine the suitability of the CD45 ptprc for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD45, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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