Immunohistochemistry (IHC)
(Immunoperoxidase staining of formalin fixed, paraffin embedded human breast cancer tissue with 214693. Low power)
Mouse anti-Human CD44v6 Monoclonal Antibody | anti-CD44v6 antibody
CD44v6 (HRP)
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
Purified
Synonyms
CD44v6; Monoclonal Antibody; CD44v6 (HRP); anti-CD44v6 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
VFF-7
Specificity
Recognizes human CD44v6.
Purity/Purification
Purified
Form/Format
Supplied as a liquid in PBS, pH 7.2. Labeled with horseradish peroxidase (HRP).
Concentration
0.8 mg/mL (varies by lot)
Applicable Applications for anti-CD44v6 antibody
Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
Immunohistochemistry (Paraffin): Requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate, pH 6.0 is recommended.
Optimal dilutions to be determined by the researcher.
Optimal dilutions to be determined by the researcher.
Immunogen
Glutathione S Transferase (GST) fusion protein corresponding to the variable domains (v3 to v10) of human CD44.
Conjugate
HRP
Hybridoma
P3X63-Ag8.653 myeloma cells with spleen cells from Balb/c mice.
Preparation and Storage
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer.
Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunohistochemistry (IHC)
(Immunoperoxidase staining of formalin fixed, paraffin embedded human breast cancer tissue with 214693. Low power)
Immunohistochemistry (IHC)
(Immunoperoxidase staining of formalin fixed, paraffin embedded human breast cancer tissue with 214693. Low power)
Immunohistochemistry (IHC)
(Immunoperoxidase staining of formalin fixed, paraffin embedded human breast cancer tissue with 214693. High power)
Immunohistochemistry (IHC)
(Immunoperoxidase staining of formalin fixed, paraffin embedded human breast cancer tissue with 214693. High power)
Product Categories/Family for anti-CD44v6 antibody
References
1. Neumayer, R. et al. (1999) CD44 expression in benign and malignant colorectal polyps. Dis. Colon. Rectum. 42: 50-55. 2. Koopman, G. et al. (1993) Activated human lymphocytes and aggressive non- Hodgkin's lymphomas express a homologue of the rat metastasis-associated variant of CD44. J. Exp. Med. 177: 897-904. 3. Yoo, S.H. et al. (2005) Expression patterns of markers for type II pneumocytes in pulmonary sclerosing hemangiomas and fetal lung tissues. Arch. Pathol. Lab. Med. 129: 915-919 4. Kim, J.S. and Werth, V.P. (2011) Identification of specific chondroitin sulfate species in cutaneous autoimmune disease. J Histochem Cytochem. 59: 780-90. 5. Chaiyarit, P. et al. (2008) Alteration of the expression of CD44 isoforms in oral epithelia and saliva from patients with oral lichen planus. J Clin Immunol.28:26-34.
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Product Notes
The CD44v6 (Catalog #AAA6250588) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The CD44v6 (HRP) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD44v6 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), Western Blot (WB). Immunohistochemistry (Paraffin): Requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate, pH 6.0 is recommended. Optimal dilutions to be determined by the researcher. Researchers should empirically determine the suitability of the CD44v6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD44v6, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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