Rat anti-Mouse CD44/Pgp-1 Monoclonal Antibody | anti-Cd44 antibody

Rat Anti-Mouse CD44/Pgp-1-LE/AF

Cat. Num. AAA673109

• Gene Names: Cd44; CD44A; METAA; RHAMM; MGC124941
• Reactivity: Mouse
• Applications: Flow Cytometry, Immunoprecipitation, Western Blot
• Synonyms: CD44/Pgp-1; Monoclonal Antibody; Rat Anti-Mouse CD44/Pgp-1-LE/AF; Rat Anti-Mouse CD44/Pgp-1 - Low Endotoxin/Azide-Free; Rat Anti-Mouse CD44/Pgp-1; anti-Cd44 antibody

• Host: Rat
• Reactivity: Mouse
• Clonality: Monoclonal
• Isotype: Rat IgG1 kappa
• Clone Number: KM201
• Specificity: All isoforms of CD44/pgp-1 (Mr 80-95 kDa); CD44 represents a family of type I transmembrane glycoproteins expressed on many cell types such as B cells, monocytes, macrophages, and variable subsets of thymocytes and peripheral T cells. CD44 primarily functions as an adhesion molecule. It has been implicated in various processes, such as hematopoiesis, lymphocyte homing, leukocyte activation, tumor metastasis and development. The KM201 monoclonal antibody reacts with an epitope very close to the hyaluronate binding domain on the CD44 molecule. KM201 can inhibit hyaluronate-dependent cell aggregation, prevent lympho-hemopoiesis in both Dexter and Whitlock- Witte Cultures, prevent the earliest intrathymic precursors from homing to the thymus, and costimulate the activation of freshly purified splenic CD^4+ T cells.^1-6
• Form/Format: Low Endotoxin, Azide - Free (LE/AF)
• Sequence Length: 780

• Applicable Applications for anti-Cd44 antibody: Flow Cytometry; Immunoprecipitation; Western Blot
• Application Notes: Research Application(s): Identification and enumeration of CD44^+ cells by flow cytometry; Studies of cell adhesion and homing
• Characterization: To ensure lot-to-lot consistency, each batch of monoclonal antibody is tested to conform to characteristics of a standard reference by flow cytometry. Representative data are included in this technical bulletin.
• Preparation and Storage: The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2-8 degree C. ; The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C. ; The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C. ; The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL or 0.2 mg in 2.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze! ; The allophycocyanin (APC) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze! ; The Spectral Red (SPRD) conjugate is supplied as 0.1 mg in 1.0 mL of PBS, pH 7.4, containing 0.1% sodium azide as preservative and a stabilizing agent. Store at 2-8 degree C. Do not freeze! ; The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below -20 degree C. ; Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide-free product at -20 degree C immediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored as directed.

References

1. Miyake, K., K. L. Medina, S. Hayashi, S. Ono, T. Hamaoka, and P. W. Kincade. 1990. J. Exp. Med. 171:477.
2. Lesley, J., Q. He, K. Miyake, A. Hamann, R. Hyman, and P. W. Kincade. 1992. J. Exp. Med. 175:257.
3. Lazaar, A. L. and E. Pure. 1995. The Immunologist 3:19.
4. Culty, M., K. Miyake, P. W. Kincade, E. Sikorski, E. C. Butcher, C. Underhill, and E. T. Silorski. 1990. J. Cell Biol. 111:2765.
5. Wu, L., P. W. Kincade, and K. Shortman. 1993. Immunol. Lett. 38:69.
6. Sommer, F., M. Huber, M. Rollinghoff, and M. Lohoff. 1996. Int. Immunol. 7:1779.
7. Murakami, S., K. Miyake, R. Abe, and P. W. Kincade. 1991. J. Immunol. 146:1422.
8. He, Q., J. Lesley, R. Hyman, K. Ishihara, and P. W. Kincade. 1992. J. Cell Biol. 119:1711.

NCBI and Uniprot Product Information

• NCBI GI #: 19923703
• NCBI GeneID: 25406
• NCBI Accession #: NP_037056.2
• NCBI GenBank Nucleotide #: NM_012924.2
• UniProt Accession #: O08779; O35249; O70509; P26051; Q9EP99; Q9ERN2; Q9ERN3
• Molecular Weight: 85,918 Da
• NCBI Official Full Name: CD44 antigen
• NCBI Official Synonym Full Names: Cd44 molecule
• NCBI Official Symbol: Cd44
• NCBI Official Synonym Symbols: CD44A; METAA; RHAMM; MGC124941
• NCBI Protein Information: CD44 antigen; PGP-1; PGP-I; HUTCH-I; ECMR-III; hermes antigen; hyaluronate receptor; phagocytic glycoprotein 1; phagocytic glycoprotein I; extracellular matrix receptor III; GP90 lymphocyte homing/adhesion receptor; Cell surface glycoprotein CD44 (hyaluronate binding protein)
• UniProt Protein Name: CD44 protein
• Protein Family: CD44 antigen
• UniProt Gene Name: Cd44
• UniProt Synonym Gene Names: CD44
• UniProt Entry Name: O08779_RAT

NCBI Description

adhesion molecule involved in migration, cell fusion and resportion in osteoclasts that also plays a role in cellular metastasis [RGD]

Uniprot Description

CD44: Receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction causes numerous pathogenic phenotypes. Great protein heterogeneity due to numerous alternative splicing and post-translational modification events. Interacts with PKN2. Interacts with HA, as well as other glycosaminoglycans, collagen, laminin, and fibronectin via its N-terminal segment. Interacts with ANK, the ERM proteins (VIL2, RDX and MSN), and NF2 via its C-terminal segment. Isoform 10 (epithelial isoform) is expressed by cells of epithelium and highly expressed by carcinomas. Expression is repressed in neuroblastoma cells. 19 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Membrane protein, integral; Receptor, misc.; Cell adhesion

Cellular Component: Golgi apparatus; focal adhesion; cell surface; protein complex; basolateral plasma membrane; apical plasma membrane; cytoplasm; plasma membrane; integral to membrane; nucleus; external side of plasma membrane

Molecular Function: hematopoietin/interferon-class (D200-domain) cytokine receptor activity; protein binding; transmembrane receptor activity; cytokine binding; hyaluronic acid binding; phosphoprotein binding; protein kinase binding; epidermal growth factor receptor binding; hyalurononglucosaminidase activity

Biological Process: cell migration; positive regulation of heterotypic cell-cell adhesion; Wnt receptor signaling pathway; positive regulation of neutrophil apoptosis; negative regulation of caspase activity; response to organic cyclic substance; blood vessel maturation; positive regulation of peptidyl-serine phosphorylation; response to vitamin A; hyaluronan catabolic process; negative regulation of DNA damage response, signal transduction by p53 class mediator; positive regulation of peptidyl-tyrosine phosphorylation; negative regulation of mature B cell apoptosis; ureteric bud branching; cartilage development; regulation of cell growth; cell adhesion; inflammatory response; healing during inflammatory response; neurite development; negative regulation of apoptosis