Rat CD44 Monoclonal Antibody | anti-CD44 antibody

Anti-Ms CD44 PE

• Gene Names: Cd44; Ly-24; Pgp-1; HERMES; AU023126; AW121933; AW146109
• Reactivity: Dog, Mouse, Human, Cat, Horse
• Applications: Flow Cytometry, Functional Assay
• Purity: The purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
• Synonyms: CD44; Monoclonal Antibody; Anti-Ms CD44 PE; PGP-I; HUTCH-I; ECMR-III; Hermes antigen; Hyaluronate receptor; Heparan sulfate proteoglycan; Epican; MC56; MIC4; CD44 molecule; anti-CD44 antibody

• Host: Rat
• Reactivity: Dog, Mouse, Human, Cat, Horse
• Clonality: Monoclonal
• Isotype: Rat IgG2b kappa
• Clone Number: IM7
• Specificity: The rat monoclonal antibody IM7 reacts with an extracellular epitope of CD44 antigen (Phagocyte glycoprotein 1), an 80-95kDa transmembrane glycoprotein (hyaladherin family) present on the most of cells and tissues (leukocytes, endothelial cells, mesenchymal cells, etc.); it is negative on platelets and hepatocytes. The antibody reacts with all isoforms of mouse CD44.
• Purity/Purification: The purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
• Form/Format: Phosphate buffered saline (PBS) solution with 15mM sodium azide
• Concentration: 0.5mg/ml (varies by lot)

• Applicable Applications for anti-CD44 antibody: Flow Cytometry (FC/FACS) (QC tested)
• Application Notes: FC/FACS: Recommended dilution: 1-3ug/ml; positive control: murine peripheral blood.
• Conjugation: PE
• Immunogen: Dexamethasone-induced cells of the SJL mouse spontaneous myeloid leukemia M1
• Preparation and Storage: Store in the dark at 2-8 degree C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.

Related Product Information for anti-CD44 antibody

CD44 is a transmembrane glycoprotein expressed on the surface of most cells, which serves as a receptor for hyaluronan. CD44 mediates angiogenesis, cell adhesion, proliferation and migration, it is thus important for lymphocyte activation, recirculation and homing, it can thus serve e.g. as a modulator of macrophage recruitment in response to pathogen. Although CD44 Functional Application (FUNC)tions are essential for physiological activities of normal cells, elevated CD44 expression correlates with poor prognosis in many carcinomas, facilitating tumour growth and metastasis, antiapoptosis and directional motility of cancer cells.

References

*Lesley J, Trowbridge IS: Genetic characterization of a polymorphic murine cell-surface glycoprotein. Immunogenetics. 1982 Mar;15(3):313-20., URL: https://www.ncbi.nlm.nih.gov/pubmed/7068175itool=,*Hegde VL, Singh NP, Nagarkatti PS, Nagarkatti M: CD44 mobilization in allogeneic dendritic cell-T cell immunological synapse plays a key role in T cell activation. J Leukoc Biol. 2008 Jul;84(1):134-42., URL: https://www.ncbi.nlm.nih.gov/pubmed/18388297itool,*van Royen N, Voskuil M, Hoefer I, Jost M, de Graaf S, Hedwig F, Andert JP, Wormhoudt TA, Hua J, Hartmann S, Bode C, Buschmann I, Schaper W, van der Neut R, Piek JJ, Pals ST: CD44 regulates arteriogenesis in mice and is differentially expressed in patients with poor and good collateralization. Circulation. 2004 Apr 6;109(13):1647-52., URL: https://www.ncbi.nlm.nih.gov/pubmed/15023889itool,*McKallip RJ, Do Y, Fisher MT, Robertson JL, Nagarkatti PS, Nagarkatti M: Role of CD44 in activation-induced cell death: CD44-deficient mice exhibit enhanced T cell response to conventional and superantigens. Int Immunol. 2002 Sep;14(9):1015-26., URL: https://www.ncbi.nlm.nih.gov/pubmed/12202399itool,*Katoh S, Matsumoto N, Kawakita K, Tominaga A, Kincade PW, Matsukura S: A role for CD44 in an antigen-induced murine model of pulmonary eosinophilia. J Clin Invest. 2003 May;111(10):1563-70., URL: https://www.ncbi.nlm.nih.gov/pubmed/12750406itool,*Legg JW, Lewis CA, Parsons M, Ng T, Isacke CM: A novel PKC-regulated mechanism controls CD44 ezrin association and directional cell motility. Nat Cell Biol. 2002 Jun;4(6):399-407., URL: https://www.ncbi.nlm.nih.gov/pubmed/12032545itool,*Si-Tahar M, Sitaraman S, Shibahara T, Madara JL: Negative regulation of epithelium-neutrophil interactions via activation of CD44. Am J Physiol Cell Physiol. 2001 Mar;280(3):C423-32., URL: https://www.ncbi.nlm.nih.gov/pubmed/11171560itool,*Larkin J, Renukaradhya GJ, Sriram V, Du W, Gervay-Hague J, Brutkiewicz RR: CD44 differentially activates mouse NK T cells and conventional T cells. J Immunol. 2006 Jul 1;177(1):268-79., URL: https://www.ncbi.nlm.nih.gov/pubmed/16785522itool,*Xu H, Manivannan A, Liversidge J, Sharp PF, Forrester JV, Crane IJ: Involvement of CD44 in leukocyte trafficking at the blood-retinal barrier. J Leukoc Biol. 2002 Dec;72(6):1133-41., URL: https://www.ncbi.nlm.nih.gov/pubmed/12488494itool

NCBI and Uniprot Product Information

• NCBI GI #: 85540468
• NCBI GeneID: 12505
• NCBI Accession #: NP_001034240.1
• NCBI GenBank Nucleotide #: NM_001039151.1
• UniProt Accession #: P15379; Q05732; Q61395; Q62060; Q62061; Q62062; Q62063; Q62408; Q62409; Q64296; Q99J14; Q9QYX8
• Molecular Weight: 39,999 Da
• NCBI Official Full Name: CD44 antigen isoform c
• NCBI Official Synonym Full Names: CD44 antigen
• NCBI Official Symbol: Cd44
• NCBI Official Synonym Symbols: Ly-24; Pgp-1; HERMES; AU023126; AW121933; AW146109
• NCBI Protein Information: CD44 antigen
• UniProt Protein Name: CD44 antigen
• Protein Family: CD44 antigen
• UniProt Gene Name: Cd44
• UniProt Synonym Gene Names: Ly-24; ECMR-III; Ly-24; PGP-1; PGP-I
• UniProt Entry Name: CD44_MOUSE

Uniprot Description

Receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. In cancer cells, may play an important role in invadopodia formation. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis (). Receptor for LGALS9; the interaction enhances binding of SMAD3 to the FOXP3 promoter, leading to up-regulation of FOXP3 expression and increased induced regulatory T (iTreg) cell stability and suppressive function (PubMed:25065622).