CD44 recombinant protein

CD44 Protein, Mouse, Recombinant (His Tag)

Cat. Num. AAA8123250

• Gene Names: Cd44; Ly-24; Pgp-1; HERMES; AU023126; AW121933; AW146109
• Purity: >95% as determined by SDS-PAGE
• Synonyms: CD44; CD44 Protein; Mouse; Recombinant (His Tag); Mouse CD44 Protein (His Tag); AU023126 Protein; AW121933 Protein; AW146109 Protein; HERMES Protein; Ly-24 Protein; Pgp-1 Protein; CD44 molecule (Indian blood group); CD44 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Thr224

• Species: Mouse
• Endotoxin: <1.0EU per ug protein as determined by the LAL method.
• Predicted N Terminal: Gln 25
• Tag: C-His
• Protein Construction: A DNA sequence encoding the mouse CD44 (NP_001034240.1)(Met1-Thr224) was expressed with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for CD44 recombinant protein

Background: CD44 is a type I transmembrane protein and a member of the cartilage link protein family. It is involved in cell-cell and cell-matrix interactions and signal transduction. Several CD44 ligands have been identified. The most extensively characterized ligand for CD44 is hyaluronan, a component of the extracellular matrix. CD44 protein is expressed on the majority of immune cells. The binding of CD44 to hyaluronan is induced on T lymphocytes after activation by antigen and on monocytes after stimulation by inflammatory agents. Under inflammatory conditions, CD44 on endothelial cells presents hyaluronan to CD44 on activated T lymphocytes and mediates a rolling interaction under flow conditions. Perturbations of the hyaluronan-CD44 interaction at the plasma membrane by various antagonists result in attenuation of receptor tyrosine kinase and transporter activities and inhibition of tumor progression in vivo. CD44 is known to interact with the ezrin family (ERM family) members and form a complex that plays diverse roles within both normal and abnormal cells, particularly cancer cells. CD44 and ezrin and their respective complex have properties suggesting that they may be important in the process of tumour-endothelium interactions, cell migrations, cell adhesion, tumour progression and metastasis.

References

Bajorath J. (2000) Molecular organization, structural features, and ligand binding characteristics of CD44, a highly variable cell surface glycoprotein with multiple functions. Proteins. 39(2): 103-11.Johnson P, et al. (2000) A role for the cell adhesion molecule CD44 and sulfation in leukocyte-endothelial cell adhesion during an inflammatory response Biochem Pharmacol. 59(5): 455-65.Martin TA, et al. (2003) The role of the CD44/ezrin complex in cancer metastasis. Crit Rev Oncol Hematol. 46(2): 165-86.Toole BP, et al. (2008) Hyaluronan, CD44 and Emmprin: partners in cancer cell chemoresistance. Drug Resist Updat. 11(3): 110-21.Johnson P, et al. (2009) CD44 and its role in inflammation and inflammatory diseases. Inflamm Allergy Drug Targets. 8(3): 208-20.

NCBI and Uniprot Product Information

• NCBI GI #: 85540468
• NCBI GeneID: 12505
• NCBI Accession #: NP_001034240.1
• NCBI GenBank Nucleotide #: NM_001039151.1
• UniProt Accession #: P15379; Q05732; Q61395; Q62060; Q62061; Q62062; Q62063; Q62408; Q62409; Q64296; Q99J14; Q9QYX8
• Molecular Weight: 39,999 Da
• NCBI Official Full Name: CD44 antigen isoform c
• NCBI Official Synonym Full Names: CD44 antigen
• NCBI Official Symbol: Cd44
• NCBI Official Synonym Symbols: Ly-24; Pgp-1; HERMES; AU023126; AW121933; AW146109
• NCBI Protein Information: CD44 antigen
• UniProt Protein Name: CD44 antigen
• Protein Family: CD44 antigen
• UniProt Gene Name: Cd44
• UniProt Synonym Gene Names: Ly-24; ECMR-III; Ly-24; PGP-1; PGP-I
• UniProt Entry Name: CD44_MOUSE

Uniprot Description

Receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. In cancer cells, may play an important role in invadopodia formation. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis (). Receptor for LGALS9; the interaction enhances binding of SMAD3 to the FOXP3 promoter, leading to up-regulation of FOXP3 expression and increased induced regulatory T (iTreg) cell stability and suppressive function (PubMed:25065622).