Rat CD41 Monoclonal Antibody | anti-CD41 antibody

RAT ANTI MOUSE CD41:RPE

Cat. Num. AAA213760

• Gene Names: Itga2b; CD41; CD41B; GpIIb; AI172977; alphaIIb
• Applications: Flow Cytometry, Functional Assay
• Synonyms: CD41; Monoclonal Antibody; RAT ANTI MOUSE CD41:RPE; anti-CD41 antibody

• Host: Rat
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: MWReg30
• Form/Format: Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
• Sequence Length: 1,033

• Applicable Applications for anti-CD41 antibody: Flow cytometry (FC/FACS)
• Application Notes: Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity fc receptors. This may be reduced by using SeroBlock FcR.; Flow Cytometry: Maximum Dilution: Neat
• Reconstitution: Reconstitute with 1 ml distilled water; Perservative Stabilisers
• Buffer Solution: Phosphate buffered saline
• Target Species: Mouse
• Preparation and Storage: Prior to reconstitution store at 4 degree C. Following reconstitution store at 4 degree C. DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 12 months from date of reconstitution.

Testing Data

(Staining of mouse peripheral blood with Rat anti Mouse CD41 (MBS212608). Platelets stain strongly for CD41)

Related Product Information for anti-CD41 antibody

Rat anti Mouse CD41 antibody, clone MWReg30 recognizes the mouse integrin alpha IIb subunit CD41. CD41 is a ~125kDa single pass type 1 transmembrane glycoprotein expressed by platelets, megakaryocytes (Zhang et al. 2007), mast cells (Berlanga et al. 2005)and hematopoietic progenitors (Mitjavila-Garcia et al. 2002). CD41 forms a heterodimer with CD61. The CD41/CD61 complex is important for platelet adhesion and aggregation (Patel et al. 2003) acting as a receptor for many extracellular matrix proteins including fibronectin, thrombospondin and vitronectin (Weisel et al. 1992). Rat anti mouse CD41, clone MWReg30 has been reported to inhibit PMA induced aggregation in vitro and to induce hypothermia in vivo (Nieswandt et al. 1999).

NCBI and Uniprot Product Information

• NCBI GI #: 159110663
• NCBI GeneID: 16399
• NCBI Accession #: NP_034705.2
• NCBI GenBank Nucleotide #: NM_010575.2
• UniProt Accession #: Q9QUM0; Q3U3R7; Q64229; Q9Z2M0
• Molecular Weight: 112,678 Da
• NCBI Official Full Name: integrin alpha-IIb
• NCBI Official Synonym Full Names: integrin alpha 2b
• NCBI Official Symbol: Itga2b
• NCBI Official Synonym Symbols: CD41; CD41B; GpIIb; AI172977; alphaIIb
• NCBI Protein Information: integrin alpha-IIb; GP IIb; GPalpha IIb; platelet glycoprotein IIb of IIb/II Ia complex; platelet membrane glycoprotein IIb
• UniProt Protein Name: Integrin alpha-IIb
• UniProt Gene Name: Itga2b
• UniProt Synonym Gene Names: GPIIb
• UniProt Entry Name: ITA2B_MOUSE

Uniprot Description

ITGA2B: Integrin alpha-IIb/beta-3 is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. It recognizes the sequence R-G-D in a wide array of ligands. It recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin alpha- IIb/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen. This step leads to rapid platelet aggregation which physically plugs ruptured endothelial cell surface. Belongs to the integrin alpha chain family. Heterodimer of an alpha and a beta subunit. The alpha subunit is composed of an heavy and a light chain linked by a disulfide bond. Alpha-IIb associates with beta-3. Directly interacts with RNF181. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion; Motility/polarity/chemotaxis; Membrane protein, integral

Cellular Component: focal adhesion; cell surface; membrane; integral to membrane; integrin complex; external side of plasma membrane

Molecular Function: identical protein binding; extracellular matrix binding; metal ion binding

Biological Process: integrin-mediated signaling pathway; cell-matrix adhesion; positive regulation of leukocyte migration; cell adhesion