Mouse CD40 Monoclonal Antibody | anti-CD40 antibody

Anti-Hu CD40 Purified

• Gene Names: Cd4; p55; W3/25
• Reactivity: Non-human primates, Human
• Applications: Immunocytochemistry, Flow Cytometry, Functional Assay, Immunohistochemistry
• Purity: Purified by protein-A affinity chromatography
• Synonyms: CD40; Monoclonal Antibody; Anti-Hu CD40 Purified; TNFRSF5; Bp50; CD40 molecule; anti-CD40 antibody

• Host: Mouse
• Reactivity: Non-human primates, Human
• Clonality: Monoclonal
• Isotype: Mouse IgG1 kappa
• Clone Number: HB14
• Specificity: The mouse monoclonal antibody HB14 recognizes an extracellular epitope of CD40 (BP50), a 48kDa type I single chain transmembrane glycoprotein expressed on normal and neoplastic B cells, but not on terminally differentiated plasma cells. CD40 antigen is also present on Hodgkin's and Reed-Sternberg cells, follicular dendritic cells, some macrophages, basal epithelial cells and endothelial cells.
• Purity/Purification: Purified by protein-A affinity chromatography
• Form/Format: Purified. Phosphate buffered saline (PBS) solution with 15mM sodium azide
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-CD40 antibody: Immunocytochemistry (ICC), Flow Cytometry (FC/FACS) (QC tested), IHC
• Application Notes: FC/FACS: Recommended dilution: 1-4ug/ml
• Immunogen: human B lymphoblastoid cell lines
• Preparation and Storage: Store at 2-8 degree C. Do not freeze. Do not use after expiration date stamped on the label.

Related Product Information for anti-CD40 antibody

CD40 is a costimulatory molecule of the TNF receptor superfamily and is expressed on many cell types, such as B cells, monocytes/macrophages, dendritic cells, endothelial cells, fibroblasts or vascular smooth muscle cells. Interaction of CD40 and its ligand CD154 (CD40L) is required for the generation of antibody responses to T-dependent antigens as well as for the development of germinal centers and memory B cells. In monocytes/macrophages CD40 engagement induces production of pro-inflammatory cytokines and chemokines. CD40-CD154 interactions are also critical for development of CD4 T cell-dependent effector Functional Application (FUNC)tions. CD40 links innate and adaptive immune responses to bacterial stimuli and serves as an important regulator affecting Functional Application (FUNC)tions of other costimulatory molecules.

References

*Leukocyte Typing V., Schlossman S. et al. (Eds.), Oxford University Press (1995)., URL:,*Kansas GS, Tedder TF: Transmembrane signals generated through MHC class II, CD19, CD20, CD39, and CD40 antigens induce LFA-1-dependent and independent adhesion in human B cells through a tyrosine kinase-dependent pathway. J Immunol. 1991 Dec 15;147(12):4094-102., URL: https://www.ncbi.nlm.nih.gov/pubmed/1721639
*Oxenius A, Campbell KA, Maliszewski CR, Kishimoto T, Kikutani H, Hengartner H, Zinkernagel RM, Bachmann MF. CD40-CD40 ligand interactions are critical in T-B cooperation but not for other anti-viral CD4+ T cell Functional Application (FUNC)tions. J Exp Med. 1996 May 1;183(5):2209-18., URL: https://www.ncbi.nlm.nih.gov/pubmed/8642330ordina,*Grewal IS, Flavell RA: The role of CD40 ligand in costimulation and T-cell activation. Immunol Rev. 1996 Oct;153:85-106., URL: https://www.ncbi.nlm.nih.gov/pubmed/9010720ordina,*Pearson LL, Castle BE, Kehry MR: CD40-mediated signaling in monocytic cells: up-regulation of tumor necrosis factor receptor-associated factor mRNAs and activation of mitogen-activated protein kinase signaling pathways. Int Immunol. 2001 Mar;13(3):273-83., URL: https://www.ncbi.nlm.nih.gov/pubmed/11222496ordin,*Wu W, Alexis NE, Chen X, Bromberg PA, Peden DB: Involvement of mitogen-activated protein kinases and NFkappaB in LPS-induced CD40 expression on human monocytic cells. Toxicol Appl Pharmacol. 2007 Dec 14, URL: https://www.ncbi.nlm.nih.gov/pubmed/18187173ordin

NCBI and Uniprot Product Information

• NCBI GI #: 6978631
• NCBI GeneID: 24932
• NCBI Accession #: NP_036837.1
• NCBI GenBank Nucleotide #: NM_012705.1
• UniProt Accession #: P05540
• Molecular Weight: 51,438 Da
• NCBI Official Full Name: T-cell surface glycoprotein CD4
• NCBI Official Synonym Full Names: Cd4 molecule
• NCBI Official Symbol: Cd4
• NCBI Official Synonym Symbols: p55; W3/25
• NCBI Protein Information: T-cell surface glycoprotein CD4; CD4 antigen; T-cell surface antigen T4/Leu-3; W3/25 antigen
• UniProt Protein Name: T-cell surface glycoprotein CD4
• Protein Family: CD40 ligand
• UniProt Gene Name: Cd4
• UniProt Entry Name: CD4_RAT

NCBI Description

MHC class II binding protein that may be a candidate gene for collagen-induced arthritis; human CD4 is a receptor required for HIV infection [RGD, Feb 2006]

Uniprot Description

Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation.