Rat anti-Canine CD4 Monoclonal Antibody | anti-CD4 antibody

CD4 (MaxLight 550)

Cat. Num. AAA6254593

• Reactivity: Canine
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry
• Purity: Purified by protein G affinity chromatography from tissue culture supernatant.
• Synonyms: CD4; Monoclonal Antibody; CD4 (MaxLight 550); anti-CD4 antibody

• Host: Rat
• Reactivity: Canine
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone Number: YKIX302.9
• Specificity: Recognizes canine CD4.
• Purity/Purification: Purified by protein G affinity chromatography from tissue culture supernatant.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight550.

• Applicable Applications for anti-CD4 antibody: Flow Cytometry (FC/FACS), Immunohistochemistry (IHC)
• Immunogen: Canine Concanavilin A activated T-cell blasts.
• Conjugate: MaxLight550
• Preparation and Storage: Store at 4 degree C. Do Not Freeze.

Testing Data

(Staining of canine peripheral blood lymphocytes with Rat anti Canine CD4: FITC)

Testing Data

(Staining of canine peripheral blood lymphocytes with Rat anti Canine CD4: Alexa Fluor® 488)

Testing Data

(Staining of canine peripheral blood lymphocytes with Rat anti Canine CD4: APC)

Product Categories/Family for anti-CD4 antibody

Antibodies; CD Markers

References

1. Cobbold, S. & Metcalfe, S. (1994) Monoclonal antibodies that define canine homologues of human CD antigens: summary of the First International Canine Leukocyte Antigen Workshop (CLAW). Tissue Antigens 43(3): 137-154. 2. Gorman, S. et al. (1994) Isolation and expression of cDNA encoding the canine CD4 and CD8a antigens.Tissue Antigens 43(3): 184-188. 3. Watson, C.J.E. et al. (1993) CD4 and CD8 monoclonal antibody therapy: strategies to prolong renal allograft survival in the dog. Brit. J. Surg. 80: 1389-1392. 4. Papadogiannakis, E.I. et al. (2009) Determination of intracellular cytokines IFN-gamma and IL-4 in canine T lymphocytes by flow cytometry following whole-blood culture.Can J Vet Res. 73: 137-143. 5. Bauer. T.R. Jr. et al. (2006) Correction of the disease phenotype in canine leukocyte adhesion deficiency using ex vivo hematopoietic stem cell gene therapy.Blood. 108: 3313-20. 6. Boggiatto, P.M. et al. (2010) Immunologic indicators of clinical progression during canine Leishmania infantum infection.Clin Vaccine Immunol. 17: 267-73. 7. Araujo, M.S. et al. (2011) Immunological changes in canine peripheral blood leukocytes triggered by immunization with first or second generation vaccines against canine visceral leishmaniasis.Vet Immunol Immunopathol. 141: 64-75. 8. Benyacoub, J. et al. (2003) Supplementation of food with Enterococcus faecium (SF68) stimulates immune functions in young dogs.J Nutr. 133: 1158-62. 9. Bund, D. et al. (2010) Canine-DCs using different serum-free methods as an approach to provide an animal-model for immunotherapeutic strategies.Cell Immunol. 263: 88-98. 10. Estrela-Lima, A. et al. (2010) Immunophenotypic features of tumor infiltrating lymphocytes from mammary carcinomas in female dogs associated with prognostic factors and survival rates.BMC Cancer. 10: 256. 11. Out, T.A. et al. (2002) Local T-cell activation after segmental allergen challenge in the lungs of allergic dogs.Immunology. 105: 499-508. 12. Pinheiro, D. (2011) Phenotypic and functional characterization of a CD4(+) CD25(high) FOXP3(high) regulatory T-cell population in the dog. Immunology. 132: 111-22. 13. Mitchell, L. et al. (2012) Clinical and immunomodulatory effects of toceranib combined with low-dose cyclophosphamide in dogs with cancer.J Vet Intern Med. 26: 355-62. 14. Tominaga, M. et al. (2010) Flow cytometric analysis of peripheral blood and tumor-infiltrating regulatory T cells in dogs with oral malignant melanoma.J Vet Diagn Invest. 22: 438-41.

NCBI and Uniprot Product Information

• NCBI GI #: 55742794
• NCBI GeneID: 403931
• NCBI Accession #: NP_001003252.1
• NCBI GenBank Nucleotide #: NM_001003252.1
• UniProt Accession #: P33705
• Molecular Weight: 51,640 Da
• NCBI Official Full Name: T-cell surface glycoprotein CD4
• NCBI Official Symbol: CD4
• NCBI Protein Information: T-cell surface glycoprotein CD4; CD4 antigen (p55); T-cell surface antigen T4/Leu-3
• UniProt Protein Name: T-cell surface glycoprotein CD4
• Protein Family: CD40 ligand
• UniProt Gene Name: CD4
• UniProt Entry Name: CD4_CANFA