Rat CD4 Monoclonal Antibody | anti-CD4 antibody

RAT ANTI MOUSE CD4

Cat. Num. AAA219512

• Gene Names: Cd4; L3T4; Ly-4
• Applications: Immunohistochemistry, ELISA, Flow Cytometry, Functional Assay, Immunofluorescence, Immunoprecipitation, Western Blot
• Synonyms: CD4; Monoclonal Antibody; RAT ANTI MOUSE CD4; anti-CD4 antibody

• Host: Rat
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone Number: GK1.5
• Form/Format: Purified; Purified IgG - liquid
• Concentration: IgG concentration 1.0 mg/ml (varies by lot)
• Sequence Length: 457

• Applicable Applications for anti-CD4 antibody: Immunohistology Frozen, ELISA (EIA), Flow cytometry (FC/FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blot (WB)
• Application Notes: Flow Cytometry: Use 10ul of the suggested working dilution to label 1x106 cells in 100ul. ; Flow Cytometry: Minimum Dilution: 1/10; Maximum Dilution: 1/200
• Perservative Stabilisers: 0.09% Sodium Azide; Preparation
• Immunogen: Murine CD4; Fusion Partners
• Target Species: Mouse
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Testing Data

(Staining of mouse spleen with Rat anti Mouse CD4 (MBS219566))

Testing Data

(Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD4 antibody, clone GK1.5 (MBS219566) followed by horseradish peroxidase conjugated Goat anti Rat IgG (MBS235195). Medium power)

Testing Data

(Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD4 antibody, clone GK1.5 (MBS219566) followed by horseradish peroxidase conjugated Goat anti Rat IgG (MBS235195). High power)

Testing Data

(Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD4 antibody, clone GK1.5 (MBS219566) followed by horseradish peroxidase conjugated Goat anti Rat IgG (MBS235195). Low power)

Testing Data

(Published customer image: Intrafollicular location of MZB cells in B6.TC spleens. A. Representative spleen sections from 3 mo old B6.TC (left) and B6 (right) mice stained with Moma-1-FITC, CD1d-PE and B220-PB. The B220+ CD1d+ MZB cells show as bright pink while the other B cells show as blue. The ring of Moma-1+ metallophillic macrophages delineates the MZ inner edge. B. Percentage of CD1d+ B220+ B cells relative to total B220+ B cells outside (MZ) and inside (FO) the Moma-1+ ring in 3 mo and 10 mo old B6 and B6.TC mice. The data show means and standard errors of the mean (SEM) calculated of 4 MZ and FO areas for each mouse. ***: p < 0.001 for t tests. C. Representative spleen sections from 10 mo old B6.TC (left) and B6 (right) mice stained with CD4-FITC, CD1d-PE and B220-PB. Boxed areas show multiple contacts between green B6.TC CD4 T cells and pink MZB cells, but not in the B6 spleen. Original magnification: 200X.From: Zhou et al. BMC Immunology 2011 12:7.)

Testing Data

(Staining of mouse peripheral blood lymphocytes with Rat anti Mouse CD4 Alexa Fluor ?647 (MBS219566A647))

Testing Data

(Staining of mouse peripheral blood lymphocytes with Rat anti Mouse CD4:FITC (MBS219462))

Testing Data

(Staining of mouse peripheral blood lymphocytes with Rat anti Mouse CD4:Low Endotoxin (MBS219398))

Testing Data

(Staining of mouse spleen with Rat anti Mouse CD4:Alexa Fluor ?488 (MBS219566A488))

Testing Data

(Staining of mouse spleen with Rat anti Mouse CD4:RPE (MBS219761))

NCBI and Uniprot Product Information

• NCBI GI #: 7304953
• NCBI GeneID: 12504
• NCBI Accession #: NP_038516.1
• NCBI GenBank Nucleotide #: NM_013488.2
• Molecular Weight: 24,473 Da
• NCBI Official Full Name: T-cell surface glycoprotein CD4
• NCBI Official Synonym Full Names: CD4 antigen
• NCBI Official Symbol: Cd4
• NCBI Official Synonym Symbols: L3T4; Ly-4
• NCBI Protein Information: T-cell surface glycoprotein CD4; T-cell differentiation antigen L3T4; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4 precursor (T-cell surface antigen T4/Leu-3) (T-cell differentiation antigen L3T4)
• UniProt Protein Name: T-cell surface glycoprotein CD4
• Protein Family: CD40 ligand
• UniProt Gene Name: Cd4
• UniProt Entry Name: CD4_MOUSE

Uniprot Description

CD4: Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts. Associates with LCK. Binds to HIV-1 gp120 and to P4HB/PDI and upon HIV-1 binding to the cell membrane, is part of P4HB/PDI- CD4-CXCR4-gp120 complex. Interacts with HIV-1 Envelope polyprotein gp160 and protein Vpu. Interacts with Human Herpes virus 7 capsid proteins. Interacts with PTK2/FAK1; this interaction requires the presence of HIV-1 gp120.

Protein type: Membrane protein, integral; Cell surface

Cellular Component: endoplasmic reticulum membrane; cell surface; membrane; endoplasmic reticulum lumen; plasma membrane; integral to membrane; lipid raft; external side of plasma membrane

Molecular Function: protein binding; protein homodimerization activity; enzyme binding; zinc ion binding; coreceptor activity; glycoprotein binding; protein kinase binding

Biological Process: maintenance of cellular protein localization; T cell activation; immune system process; cytokine production; T cell selection; positive regulation of calcium-mediated signaling; induction by virus of cell-cell fusion in host; defense response to Gram-negative bacterium; positive regulation of peptidyl-tyrosine phosphorylation; cell surface receptor linked signal transduction; positive regulation of protein kinase activity; protein palmitoleylation; immune response; positive regulation of T cell activation; cell adhesion; regulation of T cell activation; T cell differentiation