Mouse anti-Human CD4 Monoclonal Antibody | anti-CD4 antibody

Mouse Anti CD4

Cat. Num. AAA570222

• Gene Names: CD4; CD4mut
• Reactivity: Human
• Applications: Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Immunohistochemistry, Immunohistochemistry
• Synonyms: CD4; Monoclonal Antibody; Mouse Anti CD4; anti-CD4 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG
• Clone Number: Edu-2
• Specificity: CD4 (Edu-2) recognizes the CD4 antigen (a 55 kD glycoprotein).
• Form/Format: Each vial contains 1 ml of 100ug/ml purified monoclonal antibody in 0.01 M sodium phosphate, 0.15 M NaCl, 0.2% BSA, 0.09% sodium azide; pH 7.3.

• Applicable Applications for anti-CD4 antibody: Flow Cytometry (FC), Immunocytochemistry (IHC), Immunohistochemistry (IHC) (frozen), Immunohistochemistry (IHC) (paraffin)
• Application Notes: Flow cytometry and immunohistochemistry using frozen and paraffin embedded tissue sections. Optimal antibody dilution should be determined by titration. CD4 (Edu-2) is used in routine blood testing for CD4+ cells and CD4/CD8 ratios (e.g. HIV/AIDS patients) or as part of panels for the detection and differentiation of certain T cell leukemias. CD4 is also used in studies of functional activity of Th-cells in bacterial and viral infections, development of auto-immune diseases, transplant rejection, immune protection in response to allergens or allergenic reactivity.
• Source Note: Edu-2 is a mouse monoclonal IgG2a antibody raised against CD4.
• Product Note: All these reagents are effectively formulated for direct immunofluorescent staining of human tissue for flow cytometric analysis using 10 ul/106 leukocytes. Staining with clone Edu-2 (CD4) monoclonal antibodies is illustrated by flow cytometry analysis of normal blood cells. Indirect staining was performed using 10 ul of the purified monoclonal antibody with RaM FITC conjugate and 100 ul blood sample. However each investigator should titer the antibody in their application to determine the optimal per-test amount.
• Preparation and Storage: Store at 4 degree C.

Testing Data

Related Product Information for anti-CD4 antibody

CD4 (Cluster of Differentiation 4) is a glycoprotein expressed on the surface of T helper cells, monocytes, macrophages, and dendritic cells. It was discovered in the late 1970s and was originally known as leu-3 and T4 (after the OKT4 monoclonal antibody that reacted with it) before being named CD4 in 1984. In humans, the CD4 protein is encoded by the CD4 gene. CD4 plays a role in the recognition of foreign antigens presented to T cells by MHC class II molecules. Furthermore, this antigen acts as a receptor for HIV-1 by binding the viral protein gp120. The CD4 antigen is present on most thymocytes and a subpopulation of peripheral blood T cells, called T helper cells (Th). In addition, CD4 is expressed on monocytes and weak on macrophages.

Product Categories/Family for anti-CD4 antibody

CD markers

References

Carrière, D., et al., 1995, In: Leukocyte Typing V: 475-476. S.F. Schlossman, L. Boumsell, W., et al., eds. Oxford University Press, New York Piatier-Tonneau, D., et al.,1995, In: Leukocyte Typing V: 476-478. S.F. Schlossman, L. Boumsell, W. Gilks, et al., eds. Oxford University Press, New York Kaneoka, H. et al., 1983. J. Immunol., 131: 158 Lanier, L.L., et al., 1986. J. Immunol., 137: 2501 Knowles, R.W., 1986. In: Leukocyte Typing II : 259-288; E.L. Reinhert, B.F. Haynes, L.M. Nadler/ and I.D. Bernstein, eds. Springer-Verlag, New York Friedrich, W., 1982. Blood, 59: 696 Allison, J.P. and Lanier, L.L., 1987. Ann. Rev. Immunol., 5: 503

NCBI and Uniprot Product Information

• NCBI GI #: 3947700
• NCBI GeneID: 920
• UniProt Accession #: Q13969; P01730
• Molecular Weight: 7,844 Da
• NCBI Official Full Name: CD4
• NCBI Official Synonym Full Names: CD4 molecule
• NCBI Official Symbol: CD4
• NCBI Official Synonym Symbols: CD4mut
• NCBI Protein Information: T-cell surface glycoprotein CD4; CD4 receptor; CD4 antigen (p55); T-cell surface antigen T4/Leu-3
• UniProt Protein Name: CD4 protein
• Protein Family: CD40 ligand
• UniProt Gene Name: CD4
• UniProt Synonym Gene Names: CD4 receptor
• UniProt Entry Name: Q13969_HUMAN

NCBI Description

This gene encodes a membrane glycoprotein of T lymphocytes that interacts with major histocompatibility complex class II antigenes and is also a receptor for the human immunodeficiency virus. This gene is expressed not only in T lymphocytes, but also in B cells, macrophages, and granulocytes. It is also expressed in specific regions of the brain. The protein functions to initiate or augment the early phase of T-cell activation, and may function as an important mediator of indirect neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcript variants encoding different isoforms have been identified in this gene. [provided by RefSeq, Aug 2010]

Uniprot Description

CD4: Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts. Associates with LCK. Binds to HIV-1 gp120 and to P4HB/PDI and upon HIV-1 binding to the cell membrane, is part of P4HB/PDI- CD4-CXCR4-gp120 complex. Interacts with HIV-1 Envelope polyprotein gp160 and protein Vpu. Interacts with Human Herpes virus 7 capsid proteins. Interacts with PTK2/FAK1; this interaction requires the presence of HIV-1 gp120.

Protein type: Membrane protein, integral; Cell surface

Chromosomal Location of Human Ortholog: 12p13.31

Cellular Component: T cell receptor complex; endoplasmic reticulum membrane; endoplasmic reticulum lumen; early endosome; integral to membrane; plasma membrane; lipid raft; external side of plasma membrane

Molecular Function: protein binding; transmembrane receptor activity; protein homodimerization activity; enzyme binding; zinc ion binding; extracellular matrix structural constituent; coreceptor activity; receptor activity; glycoprotein binding; protein kinase binding; MHC class II protein binding

Biological Process: maintenance of cellular protein localization; viral reproduction; positive regulation of interleukin-2 biosynthetic process; T cell selection; cytokine production; positive regulation of calcium-mediated signaling; induction by virus of cell-cell fusion in host; signal transduction; defense response to Gram-negative bacterium; enzyme linked receptor protein signaling pathway; T cell receptor signaling pathway; regulation of defense response to virus by virus; positive regulation of peptidyl-tyrosine phosphorylation; cell surface receptor linked signal transduction; positive regulation of protein kinase activity; T cell costimulation; protein palmitoleylation; innate immune response; entry into host cell; immune response; transmembrane receptor protein tyrosine kinase signaling pathway; T cell differentiation; regulation of T cell activation

Disease: Okt4 Epitope Deficiency