Western Blot (WB)
(PARG Antibody (C-term) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the PARG antibody detected the PARG protein (arrow).)
Rabbit anti-Human PARG Polyclonal Antibody | anti-PARG antibody
PARG, CT (PARG, Poly(ADP-ribose) glycohydrolase) (PE)
Gene Names
PARG; PARG99
Reactivity
Human
Applications
Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
PARG; Polyclonal Antibody; CT (PARG; Poly(ADP-ribose) glycohydrolase) (PE); Poly(ADP-ribose) glycohydrolase; anti-PARG antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Applicable Applications for anti-PARG antibody
Western Blot (WB), FLISA
Application Notes
WB: 1:100-500
FLISA: 1:1,000
Applications are based on unconjugated antibody.
FLISA: 1:1,000
Applications are based on unconjugated antibody.
Immunogen
PARG antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 820-849 amino acids from the C-terminal region of human PARG.
Conjugate
PE
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(PARG Antibody (C-term) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the PARG antibody detected the PARG protein (arrow).)
Western Blot (WB)
(PARG Antibody (C-term) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the PARG antibody detected the PARG protein (arrow).)
Related Product Information for anti-PARG antibody
Poly(ADP-ribose) glycohydrolase (PARG) is the major enzyme responsible for the catabolism of poly(ADP-ribose), a reversible covalent-modifier of chromosomal proteins. The protein is found in many tissues and may be subject to proteolysis generating smaller, active products.
Product Categories/Family for anti-PARG antibody
References
Whatcott, C.J., et al. Exp. Cell Res. 315(20):3477-3485(2009). Frizzell, K.M., et al. J. Biol. Chem. 284(49):33926-33938(2009). Erdelyi, K., et al. FASEB J. 23(10):3553-3563(2009). Ame, J.C., et al. J. Cell. Sci. 122 (PT 12), 1990-2002 (2009): Uchiumi, F., et al. Genes Cells 13(12):1229-1247(2008).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
111,110 Da
NCBI Official Full Name
poly(ADP-ribose) glycohydrolase
NCBI Official Synonym Full Names
poly (ADP-ribose) glycohydrolase
NCBI Official Symbol
PARG
NCBI Official Synonym Symbols
PARG99
NCBI Protein Information
poly(ADP-ribose) glycohydrolase; poly(ADP-ribose) glycohydrolase; mitochondrial poly(ADP-ribose) glycohydrolase; poly(ADP-ribose) glycohydrolase 60 kDa isoform
UniProt Protein Name
Poly(ADP-ribose) glycohydrolase
Protein Family
UniProt Gene Name
PARG
Uniprot Description
PARG: Poly(ADP-ribose) synthesized after DNA damage is only present transiently and is rapidly degraded by poly(ADP-ribose) glycohydrolase. Poly(ADP-ribose) metabolism may be required for maintenance of the normal function of neuronal cells. Belongs to the poly(ADP-ribose) glycohydrolase family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 3.2.1.143; Hydrolase
Chromosomal Location of Human Ortholog: 10q11.23
Cellular Component: cytoplasm; cytosol; intracellular membrane-bound organelle; mitochondrial matrix; nucleoplasm; nucleus
Molecular Function: poly(ADP-ribose) glycohydrolase activity; protein binding
Biological Process: ATP generation from poly-ADP-D-ribose; carbohydrate metabolic process
Similar Products
Product Notes
The PARG parg (Catalog #AAA6330834) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PARG, CT (PARG, Poly(ADP-ribose) glycohydrolase) (PE) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PARG can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), FLISA. WB: 1:100-500 FLISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the PARG parg for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PARG, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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