Mouse anti-Feline CD4 Monoclonal Antibody | anti-CD4 antibody

Mouse Anti-Feline CD4-FITC

Cat. Num. AAA673858

• Gene Names: CD4; CD4mut
• Reactivity: Feline
• Applications: Flow Cytometry, Immunohistochemistry, Immunoprecipitation
• Synonyms: CD4; Monoclonal Antibody; Mouse Anti-Feline CD4-FITC; Mouse Anti-Feline CD4 - FITC; Mouse Anti-Feline CD4; anti-CD4 antibody

• Host: Mouse
• Reactivity: Feline
• Clonality: Monoclonal
• Isotype: Mouse IgG1 kappa
• Clone Number: 3-4F4
• Specificity: Feline CD4, Mr 65 kDa; Feline CD4, a member of the immunoglobulin superfamily of cell surface receptors, is a type I transmembrane glycoprotein that is expressed on the "helper/inducer" subpopulation of peripheral T lymphocytes. It is present on approximately 55% of thymocytes, 15% of splenocytes, 40% of lymph node cells, and 25% of peripheral blood lymphocytes.1-3 CD4 functions as an accessory molecule in the recognition of foreign antigens in association with MHC Class II antigens by T cells.
• Form/Format: Fluorescein (FITC) Conjugate
• Sequence Length: 71

• Applicable Applications for anti-CD4 antibody: Flow Cytometry; Immunohistochemistry (Acetone Fixed/Frozen Sections); Immunoprecipitation
• Application Notes: Flow Cytometry Suggested Dilution: <= 1 ug/10^6 cells
• Characterization: To insure lot to lot consistency, each batch of product is tested by flow cytometry to conform to characteristics of a standard reference reagent. Representative data are included in this product insert.
• Preparation and Storage: The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2- 8 degree C. ; The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C. ; The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C. ; The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze! ; Protect conjugated forms from light. Each reagent is stable for the period shown on the bottle label if stored as directed.

Testing Data

References

1. Ackley, C.D., E.A. Hoover, and M.D. Cooper. 1990. Tissue Antigens 35:92-98.
2. Ackley, C.D., J.K. Yamamoto, N. Levy, N.C. Pedersen, and M.D. Cooper. 1990. J. Virol. 64:5652-5655.
3. Dean, G.A., S.L. Quackenbush, C.D. Ackley, M.D. Cooper, and E.A. Hoover. 1991. Vet. Immunol. Immunopathol 28:327-335.

NCBI and Uniprot Product Information

• NCBI GI #: 3947700
• NCBI GeneID: 920
• UniProt Accession #: Q13969; P01730; Q6LCP8
• Molecular Weight: 7,844 Da
• NCBI Official Full Name: CD4
• NCBI Official Synonym Full Names: CD4 molecule
• NCBI Official Symbol: CD4
• NCBI Official Synonym Symbols: CD4mut
• NCBI Protein Information: T-cell surface glycoprotein CD4; CD4 receptor; CD4 antigen (p55); OTTHUMP00000238897; T-cell surface antigen T4/Leu-3
• UniProt Protein Name: CD4 protein
• Protein Family: CD40 ligand
• UniProt Gene Name: CD4
• UniProt Synonym Gene Names: CD4 receptor
• UniProt Entry Name: Q13969_HUMAN

NCBI Description

This gene encodes a membrane glycoprotein of T lymphocytes that interacts with major histocompatibility complex class II antigenes and is also a receptor for the human immunodeficiency virus. This gene is expressed not only in T lymphocytes, but also in B cells, macrophages, and granulocytes. It is also expressed in specific regions of the brain. The protein functions to initiate or augment the early phase of T-cell activation, and may function as an important mediator of indirect neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcript variants encoding different isoforms have been identified in this gene. [provided by RefSeq]

Uniprot Description

CD4: Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts. Associates with LCK. Binds to HIV-1 gp120 and to P4HB/PDI and upon HIV-1 binding to the cell membrane, is part of P4HB/PDI- CD4-CXCR4-gp120 complex. Interacts with HIV-1 Envelope polyprotein gp160 and protein Vpu. Interacts with Human Herpes virus 7 capsid proteins. Interacts with PTK2/FAK1; this interaction requires the presence of HIV-1 gp120.

Protein type: Membrane protein, integral; Cell surface

Chromosomal Location of Human Ortholog: 12p13.31

Cellular Component: T cell receptor complex; endoplasmic reticulum membrane; endoplasmic reticulum lumen; early endosome; plasma membrane; integral to membrane; lipid raft; external side of plasma membrane

Molecular Function: protein binding; protein homodimerization activity; transmembrane receptor activity; enzyme binding; zinc ion binding; extracellular matrix structural constituent; coreceptor activity; receptor activity; glycoprotein binding; protein kinase binding; MHC class II protein binding

Biological Process: maintenance of cellular protein localization; viral reproduction; positive regulation of interleukin-2 biosynthetic process; T cell selection; cytokine production; positive regulation of calcium-mediated signaling; induction by virus of cell-cell fusion in host; defense response to Gram-negative bacterium; signal transduction; enzyme linked receptor protein signaling pathway; T cell receptor signaling pathway; regulation of defense response to virus by virus; positive regulation of peptidyl-tyrosine phosphorylation; cell surface receptor linked signal transduction; positive regulation of protein kinase activity; protein palmitoleylation; T cell costimulation; innate immune response; immune response; entry into host cell; transmembrane receptor protein tyrosine kinase signaling pathway; regulation of T cell activation; T cell differentiation

Disease: Okt4 Epitope Deficiency