Hamster anti-Mouse CD3z Monoclonal Antibody | anti-CD3z antibody

Anti-Mouse CD3z, Biotin (Clone H146-968) (hamster IgG)

Cat. Num. AAA520100

• Gene Names: CD247; T3Z; CD3H; CD3Q; CD3Z; TCRZ; CD3-ZETA
• Reactivity: Mouse
• Applications: Immunohistochemistry, Immunofluorescence
• Synonyms: CD3z; Monoclonal Antibody; Anti-Mouse CD3z; Biotin (Clone H146-968) (hamster IgG); Biotin Anti-Mouse CD3z (CD247) Monoclonal Antibody; anti-CD3z antibody

• Host: Hamster
• Reactivity: Mouse
• Clonality: Monoclonal
• Isotype: Hamster IgG2kappa
• Clone Number: H146-968
• Specificity: CD3z (CD247) (Mouse CD3z (zeta chain); also known as CD247)
• Form/Format: Biotin (Biotin conjugated Ig buffered in PBS, 0.02% NaN3 and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/ml. (Purified from ascitic fluid via Protein G Chromatography))
• Concentration: Antibody Concentration: 0.1 mg/ml (varies by lot)
• Sequence Length: 164

• Applicable Applications for anti-CD3z antibody: Immunohistochemistry (IHC), Immunofluorescence (IF)
• Immunogen: KLH conjugated mouse zeta-chain (CD3) sequence 151-164
• Donor: Armenian hamster
• Presentation: 100 ug or 300 ug Bitoin conjugated Ig buffered in PBS, 0.02% NaN3 and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/ml.
• Preparation and Storage: Stable at 4 degree C. For long term storage, aliquot and freeze unused portions at -20 degree C in volumes appropriate for single usage. Avoid repeated freeze/thaw cycles.

Related Product Information for anti-CD3z antibody

The TCR complex consists of a ligand specific alpha/beta heterodimer non-covalently associated with five invariant chains including the CD3 gamma, delta, eta and zeta subunits. This complex regulates assembly and expression of the receptor and is thought to be responsible for transmembrane transduction of signals after binding to TCRalphabeta. Studies have suggested that the CD3z subunit (also know as CD247) plays an important role in two distinct cellular compartments. In the cytoplasm, it may function to regulate Ag receptor expression and in the plasma membrane it may be required for optimal signaling by the physiologic ligand Ag/MHC. The CD3z subunit is also associated with the CD16 IgG Fc receptor on NK cells. This clone (H146-968) has been reported to work in flow cytometry (in permeabilized cells), Western Blotting and ELISA.

NCBI and Uniprot Product Information

• NCBI GI #: 66841741
• NCBI GeneID: 919
• UniProt Accession #: P20963; Q5VX13; Q8TAX4; B1AK49; A46461
• Molecular Weight: 18,696 Da
• NCBI Official Full Name: CD3Z antigen, zeta polypeptide (TiT3 complex)
• NCBI Official Synonym Full Names: CD247 molecule
• NCBI Official Symbol: CD247
• NCBI Official Synonym Symbols: T3Z; CD3H; CD3Q; CD3Z; TCRZ; CD3-ZETA
• NCBI Protein Information: T-cell surface glycoprotein CD3 zeta chain; CD3zeta chain; TCR zeta chain; CD247 antigen, zeta subunit; T-cell receptor T3 zeta chain; CD3Z antigen, zeta polypeptide (TiT3 complex); T-cell antigen receptor complex, zeta subunit of CD3
• UniProt Protein Name: T-cell surface glycoprotein CD3 zeta chain
• UniProt Gene Name: CD247
• UniProt Synonym Gene Names: CD3Z; T3Z; TCRZ
• UniProt Entry Name: CD3Z_HUMAN

NCBI Description

The protein encoded by this gene is T-cell receptor zeta, which together with T-cell receptor alpha/beta and gamma/delta heterodimers, and with CD3-gamma, -delta and -epsilon, forms the T-cell receptor-CD3 complex. The zeta chain plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. Low expression of the antigen results in impaired immune response. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Probable role in assembly and expression of the TCR complex as well as signal transduction upon antigen triggering.

Subunit structure: The TCR/CD3 complex of T-lymphocytes consists of either a TCR alpha/beta or TCR gamma/delta heterodimer coexpressed at the cell surface with the invariant subunits of CD3 labeled gamma, delta, epsilon, zeta, and eta. CD3-zeta forms either homodimers or heterodimers with CD3-eta. Interacts with SLA and SLA2. Interacts with DOCK2 and TRAT1. Interacts with HIV-1; this interaction up-regulates the expression of the Fas ligand (FASLG) at the cell surface. Interacts with HIV-2 Nef protein; this interaction induces down-regulation of cell surface TCR/CD3 complexes. Interacts with SHB. Interacts with ZAP70. Interacts (tyrosine phosphorylated) with SHC1 (via SH2 domain). Interacts with PTPRC. Ref.7 Ref.8 Ref.9 Ref.10 Ref.11 Ref.12 Ref.15 Ref.20 Ref.21 Ref.22 Ref.23

Subcellular location: Membrane; Single-pass type I membrane protein.

Domain: The ITAM domains mediate interaction with SHB.

Post-translational modification: Phosphorylated on Tyr residues after T-cell receptor triggering

By similarity.

Involvement in disease: Immunodeficiency due to defect in CD3-zeta (CD3ZID) [MIM:610163]: An immunological deficiency characterized by T-cells impaired immune response to alloantigens, tetanus toxoid and mitogens.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.19

Sequence similarities: Belongs to the CD3Z/FCER1G family.Contains 3 ITAM domains.