Rabbit anti-Human CD3D  Monoclonal Antibody | anti-CD3D  antibody

Rabbit anti Human CD3D  Monoclonal Antibody

Cat. Num. AAA2544132

• Gene Names: CD3D; T3D; IMD19; CD3-DELTA
• Reactivity: Human
• Applications: Western Blot, ELISA, Immunoprecipitation
• Synonyms: CD3D ; Monoclonal Antibody; Rabbit anti Human CD3D Monoclonal Antibody; anti-CD3D antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG
• Sequence Length: 183

• Applicable Applications for anti-CD3D antibody: Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Application Notes: WB: 10-20 mug/mL; ELISA: 0.1-0.2 mug/mL. This antibody can be used at 0.1-0.2 mug/mL with the appropriate secondary reagents to detect Human CD3D. The detection limit for Human CD3D is 0.00245 ng/well.; IP: 4-6 mug/mg of lysate
• Immunogen: Recombinant Human CD3D protein
• Buffer: 0.2 mum filtered solution in PBS with 5% trehalose
• Preparation and Storage: This antibody can be stored at 2 degree C- 8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Related Product Information for anti-CD3D  antibody

T-cell surface glycoprotein CD3 delta chain, also known as CD3D, is a single-pass type I membrane protein. CD3D, together with CD3-gamma, CD3-epsilon and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. The majority of T cell receptor (TCR) complexes in mice and humans consist of a heterodimer of polymorphic TCRalpha and beta chains along with invariant CD3gamma, delta, epsilon, and zeta chains. CD3 chains are present as CD3gammaepsilon, deltaepsilon, and zetazeta dimers in the receptor complex and play critical roles in the antigen receptor assembly, transport to the cell surface, and the receptor-mediated signal transduction. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. This complex is critical for T-cell development and function, and represents one of the most complex transmembrane receptors. The T cell receptor-CD3 complex is unique in having ten cytoplasmic immunoreceptor tyrosine-based activation motifs (ITAMs). CD3D contains 1 ITAM domain and has been shown to interact with CD8A. In the mouse, knockout of CD3delta allows some degree of T lymphocyte differentiation since mature CD4 and CD8 as well as TCRgammadelta T lymphocytes are observed in the periphery. In contrast, deleterious mutation of the CD3delta encoding gene in the human leads to a severe combined immunodeficiency characterised by the complete absence of mature T cell subpopulations including TCRalpha/beta and TCRgamma/delta. Defects in CD3D cause severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T-/B+/NK+ SCID) which is a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. In humans the absence of CD3 delta results in a complete arrest in thymocyte development at the stage of double negative to double positive transition and the development of gamma delta T-cell receptor-positive T cells is also impaired.

NCBI and Uniprot Product Information

• NCBI GI #: 119587770
• NCBI GeneID: 915
• Molecular Weight: 14,484 Da
• NCBI Official Full Name: CD3d antigen, delta polypeptide (TiT3 complex), isoform CRA_b
• NCBI Official Synonym Full Names: CD3d molecule
• NCBI Official Symbol: CD3D
• NCBI Official Synonym Symbols: T3D; IMD19; CD3-DELTA
• NCBI Protein Information: T-cell surface glycoprotein CD3 delta chain
• UniProt Protein Name: T-cell surface glycoprotein CD3 delta chain
• UniProt Gene Name: CD3D
• UniProt Synonym Gene Names: T3D
• UniProt Entry Name: CD3D_HUMAN

NCBI Description

The protein encoded by this gene is part of the T-cell receptor/CD3 complex (TCR/CD3 complex) and is involved in T-cell development and signal transduction. The encoded membrane protein represents the delta subunit of the CD3 complex, and along with four other CD3 subunits, binds either TCR alpha/beta or TCR gamma/delta to form the TCR/CD3 complex on the surface of T-cells. Defects in this gene are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (SCIDBNK). Two transcript variants encoding different isoforms have been found for this gene. Other variants may also exist, but the full-length natures of their transcripts has yet to be defined. [provided by RefSeq, Feb 2009]

Uniprot Description

CD3D: a T cell surface glycoprotein that is a component of the T cell antigen receptor. A defect in this protein causes a severe combined immunodeficiency characterized by the absence of T cells but normal B cells. Contains 1 ITAM domain.

Protein type: Membrane protein, integral; Receptor, misc.

Chromosomal Location of Human Ortholog: 11q23

Cellular Component: plasma membrane; T cell receptor complex

Molecular Function: protein heterodimerization activity; transcription coactivator activity; transmembrane receptor activity

Biological Process: cell surface receptor linked signal transduction; positive regulation of transcription from RNA polymerase II promoter; positive thymic T cell selection; regulation of immune response; T cell costimulation; T cell differentiation; T cell receptor signaling pathway

Disease: Immunodeficiency 19