Rabbit anti-Human CD3D Monoclonal Antibody | anti-CD3D antibody

Recombinant Anti-CD3D Antibody, Rabbit Monoclonal

Cat. Num. AAA8104500

• Gene Names: CD3D; T3D; IMD19; CD3-DELTA
• Reactivity: Human
• Applications: Western Blot, ELISA, Immunoprecipitation
• Purity: Protein A
• Synonyms: CD3D; Monoclonal Antibody; Recombinant Anti-CD3D Antibody; Rabbit Monoclonal; CD3d/CD3 delta Antibody; Rabbit MAb; CD3d molecule; delta (CD3-TCR complex); Anti-CD3 delta Antibody; Anti-CD3-DELTA Antibody; Anti-IMD19 Antibody; Anti-T3D Antibody; anti-CD3D antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Rabbit IgG
• Clone Number: 6
• Specificity: Human CD3D/CD3 delta chain
• Purity/Purification: Protein A
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-CD3D antibody: Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:1000; ELISA: 1:25,000-1:50,000; IP: 1-2uL/mg of lysate
• Antibody Type: Recombinant Antibody
• Immunogen: Recombinant Human CD3D protein
• Conjugation: Unconjugated
• Preparation: This antibody was obtained from a rabbit immunized with purified, recombinant Human CD3D (rh CD3D).
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunoprecipitation (IP)

(CD3D was immunoprecipitated using:Lane A:0.5 mg HuT78 Whole Cell Lysate0.5 uL anti-CD3D rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-CD3D rabbit monoclonal antibody,at 1:500 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 20 kDaObserved band size: 20 kDa)

Western Blot (WB)

(Anti-CD3D rabbit monoclonal antibody at 1:500 dilutionLane A: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:19 kDaObserved band size:25 kDa(We are unsure as to the identity of these extra bands.))

Related Product Information for anti-CD3D antibody

T-cell surface glycoprotein CD3 delta chain, also known as CD3D, is a single-pass type I membrane protein. CD3D, together with CD3-gamma, CD3-epsilon and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. The majority of T cell receptor (TCR) complexes in mice and humans consist of a heterodimer of polymorphic TCRalpha and beta chains along with invariant CD3gamma, delta, epsilon, and zeta chains. CD3 chains are present as CD3gammaepsilon, deltaepsilon, and zetazeta dimers in the receptor complex and play critical roles in the antigen receptor assembly, transport to the cell surface, and the receptor-mediated signal transduction. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. This complex is critical for T-cell development and function, and represents one of the most complex transmembrane receptors. The T cell receptor-CD3 complex is unique in having ten cytoplasmic immunoreceptor tyrosine-based activation motifs (ITAMs). CD3D contains 1 ITAM domain and has been shown to interact with CD8A. In the mouse, knockout of CD3delta allows some degree of T lymphocyte differentiation since mature CD4 and CD8 as well as TCRgammadelta T lymphocytes are observed in the periphery. In contrast, deleterious mutation of the CD3delta encoding gene in the human leads to a severe combined immunodeficiency characterised by the complete absence of mature T cell subpopulations including TCRalpha/beta and TCRgamma/delta. Defects in CD3D cause severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T-/B+/NK+ SCID) which is a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. In humans the absence of CD3 delta results in a complete arrest in thymocyte development at the stage of double negative to double positive transition and the development of gamma delta T-cell receptor-positive T cells is also impaired.

Product Categories/Family for anti-CD3D antibody

T Cell Receptor Signaling Pathway

References

Roifman CM. (2004) CD3 delta immunodeficiency. Curr Opin Allergy Clin Immunol. 4(6): 479-84.
Pan Q, et al. (2006) Different role for mouse and human CD3delta/epsilon heterodimer in preT cell receptor (preTCR) function: human CD3delta/epsilon heterodimer restores the defective preTCR function in CD3gamma-and CD3gammadelta-deficient mice. Mol Immunol. 43(11): 1741-50.
Le Deist F, et al. (2007) Expression anomalies of the CD3-TCR complex expression and immunodeficiencies. Med Sci (Paris). 23(2): 161-6.

NCBI and Uniprot Product Information

• NCBI GI #: 4502669
• NCBI GeneID: 915
• NCBI Accession #: NP_000723.1
• NCBI GenBank Nucleotide #: NM_000732.4
• Molecular Weight: 14,484 Da
• NCBI Official Full Name: T-cell surface glycoprotein CD3 delta chain isoform A
• NCBI Official Synonym Full Names: CD3d molecule
• NCBI Official Symbol: CD3D
• NCBI Official Synonym Symbols: T3D; IMD19; CD3-DELTA
• NCBI Protein Information: T-cell surface glycoprotein CD3 delta chain
• Protein Family: T-cell surface glycoprotein

NCBI Description

The protein encoded by this gene is part of the T-cell receptor/CD3 complex (TCR/CD3 complex) and is involved in T-cell development and signal transduction. The encoded membrane protein represents the delta subunit of the CD3 complex, and along with four other CD3 subunits, binds either TCR alpha/beta or TCR gamma/delta to form the TCR/CD3 complex on the surface of T-cells. Defects in this gene are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (SCIDBNK). Two transcript variants encoding different isoforms have been found for this gene. Other variants may also exist, but the full-length natures of their transcripts has yet to be defined. [provided by RefSeq, Feb 2009]