Mouse anti-Human, Mouse CD3D Monoclonal Antibody | anti-CD3D antibody

CD3D Monoclonal Antibody

Cat. Num. AAA2542249

• Gene Names: CD3D; T3D; IMD19; CD3-DELTA
• Reactivity: Human, Mouse
• Applications: Western Blot, ELISA
• Purity: Protein G purification
• Synonyms: CD3D; Monoclonal Antibody; CD3D Monoclonal Antibody; CD3 antigen delta subunit; CD3 delta; CD3-delta; CD3d; CD3d antigen delta polypeptide (TiT3 complex); CD3d molecule; CD3d molecule delta (CD3-TCR complex); IMD19; OKT3 delta chain; T cell receptor T3 delta chain; T-cell receptor T3 delta chain; T-cell surface glycoprotein CD3 delta chain; T3D; anti-CD3D antibody

• Host: Mouse
• Reactivity: Human, Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 222
• Purity/Purification: Protein G purification
• Concentration: 1mg/mL (varies by lot)
• Sequence Length: 171

• Applicable Applications for anti-CD3D antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:200-1:2000
• Immunogen: Fusion protein of CD3 delta
• Buffer: PBS with 0.02% sodium azide, 50% glycerol, PH7.3
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C. Avoid freeze / thaw cycles.

Western Blot (WB)

(Western Blot analysis of Jurkat cells using CD3 delta Monoclonal Antibody at dilution of 1:500)

Related Product Information for anti-CD3D antibody

CD3 is a complex of proteins that directly associates with the T cell receptor (TCR). The TCR/CD3 complex of T-lymphocytes consists of either a TCR alpha/beta or TCR gamma/delta heterodimer coexpressed at the cell surface with the invariant subunits of CD3 labeled gamma, delta, epsilon, zeta, and eta. The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction. CD3 is considered to be a pan-T cell marker for detection of normal and neoplastic T cells.

Product Categories/Family for anti-CD3D antibody

Cancer; Immunology; Stem cells

NCBI and Uniprot Product Information

• NCBI GI #: 4502669
• NCBI GeneID: 915
• NCBI Accession #: NP_000723.1
• NCBI GenBank Nucleotide #: NM_000732.4
• UniProt Accession #: P04234; A8MVP6
• Molecular Weight: Calculated: 19kDa; Observed: 25-30kDa
• NCBI Official Full Name: T-cell surface glycoprotein CD3 delta chain isoform A
• NCBI Official Synonym Full Names: CD3d molecule
• NCBI Official Symbol: CD3D
• NCBI Official Synonym Symbols: T3D; IMD19; CD3-DELTA
• NCBI Protein Information: T-cell surface glycoprotein CD3 delta chain
• UniProt Protein Name: T-cell surface glycoprotein CD3 delta chain
• Protein Family: T-cell surface glycoprotein
• UniProt Gene Name: CD3D
• UniProt Synonym Gene Names: T3D

NCBI Description

The protein encoded by this gene is part of the T-cell receptor/CD3 complex (TCR/CD3 complex) and is involved in T-cell development and signal transduction. The encoded membrane protein represents the delta subunit of the CD3 complex, and along with four other CD3 subunits, binds either TCR alpha/beta or TCR gamma/delta to form the TCR/CD3 complex on the surface of T-cells. Defects in this gene are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (SCIDBNK). Two transcript variants encoding different isoforms have been found for this gene. Other variants may also exist, but the full-length natures of their transcripts has yet to be defined. [provided by RefSeq, Feb 2009]

Uniprot Description

Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways (PubMed:2470098). In addition of this role of signal transduction in T-cell activation, CD3D plays an essential role in thymocyte differentiation. Indeed, participates in correct intracellular TCR-CD3 complex assembly and surface expression. In absence of a functional TCR-CD3 complex, thymocytes are unable to differentiate properly. Interacts with CD4 and CD8 and thus serves to establish a functional link between the TCR and coreceptors CD4 and CD8, which is needed for activation and positive selection of CD4 or CD8 T-cells(PubMed:12215456).