Mouse anti-Human CD39 Monoclonal Antibody | anti-CD39 antibody

anti-human CD39-Purified (with Antibiotics)

Cat. Num. AAA666066

• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Western Blot, Immunoprecipitation
• Purity: Purified (with Antibiotics)
• Synonyms: CD39; Monoclonal Antibody; anti-human CD39-Purified (with Antibiotics); Monoclonal anti-human CD39; anti-CD39 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: BU61
• Purity/Purification: Purified (with Antibiotics)
• Form/Format: 50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 0.5% Gentamicin sulfate
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 513

• Applicable Applications for anti-CD39 antibody: Flow Cytometry (FC/FACS), Western Blot (WB), Immunoprecipitation (IP)
• Performance: Five x 105 ficoll prepared human peripheral blood mononuclear cells were washed and pre incubated ~5 minutes with 20ul of 300 ug/ml human IgG (to block non specific binding) after which they were incubated 45 minutes on ice with 80 ul of anti-CD39 at 5 ug/ml. Cells were washed twice and incubated with 2 degree reagent Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed by FACS. A 15% sub population of the cells stained positive with a mean shift of 1.25 log10 fluorescent units when compared to a Mouse IgG1 negative control at a similar concentration.
• Production: Antibody was Protein A purified from (low FBS containing) tissue culture supernatant. Purity was >95% Immunoglobulin by SDS-PAGE and contains less than 1% Bovine Immunoglobulin.
• Buffer: 50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 0.5 mg/ml Gentamicin Sulfate (as a preservative).
• Group Header: anti-human CD39
• Immunogen: Human WM-1 (Waldenström’s macroglobulinemia) cell line
• Preparation and Storage: Store at 2 - 5 degree C. Freeze/Thawing is not recommended.; Product should retain activity for at least 12 months after shipping date when stored as recommended.

Testing Data

Related Product Information for anti-CD39 antibody

Information: Human CD39 is found on most mature B cells, activated NK cells and activated T cells. CD39 has homology to the N- myc family of proteins and has been cloned. Antibody BU61 recognizes the CD39 molecule of approximately 80 kd.

References

J.R. Voland, et al, (1992) Proc Natl Acad Sci USA 89: 10425-10429. C.R. Maliszewski, et al, (1994) J Immunol 153: 3574-3583. Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford, (1995) p. 383-385.

NCBI and Uniprot Product Information

• NCBI GI #: 2240205
• NCBI GeneID: 282223
• Molecular Weight: 58,114 Da
• NCBI Official Full Name: CD39
• NCBI Official Synonym Full Names: ectonucleoside triphosphate diphosphohydrolase 1<
• NCBI Official Symbol: ENTPD1
• NCBI Protein Information: ectonucleoside triphosphate diphosphohydrolase 1; ATPDase; NTPDase 1; ecto-apyrase; ecto-ATPase 1; ecto-ATPDase 1; ecto-ATP diphosphohydrolase 1; lymphoid cell activation antigen
• UniProt Protein Name: Ectonucleoside triphosphate diphosphohydrolase 1
• UniProt Gene Name: ENTPD1
• UniProt Synonym Gene Names: CD39; NTPDase 1; Ecto-ATPDase 1; Ecto-ATPase 1
• UniProt Entry Name: ENTP1_BOVIN

Uniprot Description

In the nervous system, could hydrolyze ATP and other nucleotides to regulate purinergic neurotransmission. Could also be implicated in the prevention of platelet aggregation by hydrolyzing platelet-activating ADP to AMP. Hydrolyzes ATP and ADP equally well.