Mouse CD38 Monoclonal Antibody | anti-CD38 antibody

anti-human CD38-FITC

Cat. Num. AAA666491

• Gene Names: CD38; T10; ADPRC 1
• Reactivity: Human, Cynomolgus
• Applications: Flow Cytometry, Functional Assay
• Synonyms: CD38; Monoclonal Antibody; anti-human CD38-FITC; Monoclonal anti-human CD38/Biotin; anti-CD38 antibody

• Host: Mouse
• Reactivity: Human, Cynomolgus
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: AT1
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 300

• Applicable Applications for anti-CD38 antibody: Flow Cytometry (FC/FACS)
• Application Notes: Working dilution : 1:50 (or use 1.6 uL of concentrated stock per 5 x 10⁵-cell test)
• Performance: Five x 10⁵ cultured human Raji cells were washed and incubated 45 minutes on ice with 80 ul of anti-CD38/Biotin at 5 ug/ml. Cells were washed twice and incubated with 2 degree reagent Streptavidin/R-PE after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.94 log₁₀ fluorescent units when compared to a Mouse IgG1/Biotin negative control (MBS666714) at a similar concentration. Binding was blocked when cells were pre incubated 10 minutes with 20 ul of 0.5 mg/ml anti-CD38 antibody (MBS666469).
• Buffer: 50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA, 0.04% NaN₃ (as a preservative).
• Conjugation: FITC
• Production: Protein A purified antibody from tissue culture supernatant was reacted with FITC. Unconjugated FITC was separated from antibody/FITC conjugate by desalting column. The antibody/FITC conjugate is at 0.25 mg/mL with a Fluorescein/IgG molar ratio of 7.1.
• Preparation and Storage: Store at 2 - 5°C. ; Freeze/thawing not recommended.; Protect from light; ; Product should retain activity for at least 12 months after shipping date when stored as recommended.

Testing Data

(Binding of anti-CD38/FITC to human Raji cells)

Related Product Information for anti-CD38 antibody

Information: Human CD38 is a type II transmembrane glycoprotein with bifunctional ectoenzyme activity catalyzing both synthesis and hydrolysis of cyclic ADP ribose (1). CD38 is involved in lymphocyte activation and adhesion to endothelium and has recently been identified as a counter-receptor of CD31 (2). Antibody AT1 recognizes the CD38 molecule of 45 kd.

References

1.) Leukocyte Typing VI (T. Kishimoto, et al, eds.) Garland Publishing, Inc., New York (1997) p. 151-157. 2.) S. Deaglio, et al, (1998) J Immunol 160:395-402.

NCBI and Uniprot Product Information

• NCBI GI #: 1911103
• NCBI GeneID: 952
• NCBI Official Full Name: CD38
• NCBI Official Synonym Full Names: CD38 molecule
• NCBI Official Symbol: CD38
• NCBI Official Synonym Symbols: T10; ADPRC 1
• NCBI Protein Information: ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1; cADPr hydrolase 1; CD38 antigen (p45); NAD(+) nucleosidase; ADP-ribosyl cyclase 1; 2'-phospho-ADP-ribosyl cyclase; 2'-phospho-cyclic-ADP-ribose transferase; 2'-phospho-ADP-ribosyl cyclase/2'-phospho-cyclic-ADP-ribose transferase
• UniProt Protein Name: ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1
• Protein Family: ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase
• UniProt Gene Name: CD38
• UniProt Synonym Gene Names: ADPRC 1; cADPr hydrolase 1
• UniProt Entry Name: CD38_HUMAN

NCBI Description

CD38 is a novel multifunctional ectoenzyme widely expressed in cells and tissues especially in leukocytes. CD38 also functions in cell adhesion,signal transduction and calcium signaling. [provided by RefSeq, Jul 2008]

Uniprot Description

CD38: Synthesizes cyclic ADP-ribose, a second messenger for glucose-induced insulin secretion. Also has cADPr hydrolase activity. Also moonlights as a receptor in cells of the immune system. Belongs to the ADP-ribosyl cyclase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.2.2.6; Membrane protein, integral; Lyase; Apoptosis; EC 2.4.99.20; Cofactor and Vitamin Metabolism - nicotinate and nicotinamide; Cell cycle regulation; Hydrolase; Cell surface

Chromosomal Location of Human Ortholog: 4p15

Cellular Component: cell surface; membrane; plasma membrane; integral to membrane; nucleus

Molecular Function: transferase activity; phosphorus-oxygen lyase activity; NAD+ nucleosidase activity; NAD(P)+ nucleosidase activity

Biological Process: response to drug; response to retinoic acid; metabolic process; positive regulation of transcription, DNA-dependent; positive regulation of insulin secretion; female pregnancy; signal transduction; positive regulation of cell growth; response to estradiol stimulus; elevation of cytosolic calcium ion concentration; B cell receptor signaling pathway; response to hydroperoxide; positive regulation of B cell proliferation; response to hypoxia; positive regulation of vasoconstriction; response to progesterone stimulus; negative regulation of transcription, DNA-dependent; negative regulation of bone resorption; negative regulation of apoptosis