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Testing Data (Staining of Mouse peripheral blood platelets with Rat anti Mouse CD36:Alexa Fluor ?647 (MBS217380A647))

Rat CD36 Monoclonal Antibody | anti-CD36 antibody

RAT ANTI MOUSE CD36

Gene Names
Cd36; FAT; GPIV; Scarb3
Applications
Flow Cytometry, Functional Assay, Immunofluorescence, Immunoprecipitation, Western Blot
Synonyms
CD36; Monoclonal Antibody; RAT ANTI MOUSE CD36; anti-CD36 antibody
Ordering
For Research Use Only!
Host
Rat
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
MF3
Form/Format
Purified
Purified IgG - liquid
Concentration
IgG concentration 1.0mg/ml (varies by lot)
Sequence Length
472
Applicable Applications for anti-CD36 antibody
Flow cytometry (FC/FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blot (WB)
Application Notes
Flow Cytometry: Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Flow Cytometry: Minimum Dilution: Neat; Maximum Dilution: 1/25
Perservative Stabilisers
0.09% Sodium Azide (NaN3)
Preparation
Immunogen
IL-4 treated murine thioglycollate-elicited peritoneal macrophages.
Fusion Partners
Target Species
Mouse
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.

Testing Data

(Staining of Mouse peripheral blood platelets with Rat anti Mouse CD36:Alexa Fluor ?647 (MBS217380A647))

Testing Data (Staining of Mouse peripheral blood platelets with Rat anti Mouse CD36:Alexa Fluor ?647 (MBS217380A647))

Testing Data

(Staining of mouse peripheral blood platelets with Rat anti Mouse CD36 (MBS217382))

Testing Data (Staining of mouse peripheral blood platelets with Rat anti Mouse CD36 (MBS217382))

Testing Data

(Staining of Mouse peripheral blood platelets with Rat anti Mouse CD36 Alexa Fluor 488 (MBS217380A488))

Testing Data (Staining of Mouse peripheral blood platelets with Rat anti Mouse CD36 Alexa Fluor 488 (MBS217380A488))

Testing Data

(Staining of mouse peripheral blood platelets with Rat anti Mouse CD36:Low Endotoxin (MBS217381))

Testing Data (Staining of mouse peripheral blood platelets with Rat anti Mouse CD36:Low Endotoxin (MBS217381))

Testing Data

(Staining of mouse peripheral blood platelets with Rat anti Mouse CD36)

Testing Data (Staining of mouse peripheral blood platelets with Rat anti Mouse CD36)

Testing Data

(Staining of mouse peripheral blood platelets with Rat anti Mouse CD36:FITC (MBS219350))

Testing Data (Staining of mouse peripheral blood platelets with Rat anti Mouse CD36:FITC (MBS219350))
Related Product Information for anti-CD36 antibody
Rat anti Mouse CD36 antibody, clone MF3 recognizes mouse CD36, an 85kD transmembrane glycoprotein which is primarily expressed on platelets, monocytes/macrophages, smooth muscle and endothelial cells. The CD36 molecule is type B scavenger receptor, which binds to multiple ligands including thrombospondin, anionic phospholipids, oxidized low density lipoproteins and long chain fatty acids. CD36 has diverse functions and is reported to play a role in innate immunity, platelet adhesion/aggregation and long chain fatty acid transport. The CD36 molecule also directly mediates cytoadhesion of erythrocytes infected with Plasmodium falciparum, and may be involved in the development of atherosclerotic lesions and the formation of foam cells. Rat anti Mouse CD36 antibody, clone MF3 has been shown to inhibit IL-4 induced thioglycollate-elicited peritoneal macrophage fusion and significantly block IL-4/GM-CSF-induced bone-marrorw derived macrophage fusion.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
52,698 Da
NCBI Official Full Name
platelet glycoprotein 4
NCBI Official Synonym Full Names
CD36 antigen
NCBI Official Symbol
Cd36
NCBI Official Synonym Symbols
FAT; GPIV; Scarb3
NCBI Protein Information
platelet glycoprotein 4; GPIIIB; PAS IV; PAS-4; fatty acid translocase; glycoprotein IIIb; platelet glycoprotein IV
UniProt Protein Name
Platelet glycoprotein 4
Protein Family
UniProt Gene Name
Cd36
UniProt Synonym Gene Names
GPIIIB; GPIV
UniProt Entry Name
CD36_MOUSE

Uniprot Description

CD36: Seems to have numerous potential physiological functions. Binds to collagen, thrombospondin, anionic phospholipids and oxidized LDL. May function as a cell adhesion molecule. Directly mediates cytoadherence of Plasmodium falciparum parasitized erythrocytes. Binds long chain fatty acids and may function in the transport and/or as a regulator of fatty acid transport. Receptor for thombospondins, THBS1 AND THBS2, mediating their antiangiogenic effects. Defects in CD36 are the cause of platelet glycoprotein IV deficiency (PG4D)[MIM:608404]; also known as CD36 deficiency. Platelet glycoprotein IV deficiency can be divided into 2 subgroups. The type I phenotype is characterized by platelets and monocytes/macrophages exhibiting complete CD36 deficiency. The type II phenotype lacks the surface expression of CD36 in platelets, but expression in monocytes/macrophages is near normal. Genetic variations in CD36 are associated with susceptibility to coronary heart disease type 7 (CHDS7). Belongs to the CD36 family.

Protein type: Membrane protein, integral; Cell adhesion; Membrane protein, multi-pass

Cellular Component: Golgi apparatus; extracellular space; cell surface; protein complex; intracellular membrane-bound organelle; mitochondrion; endoplasmic reticulum; integral to membrane; caveola; lipid raft; membrane; apical part of cell; plasma membrane; intracellular; sarcolemma; external side of plasma membrane

Molecular Function: low-density lipoprotein receptor activity; protein binding; transforming growth factor beta binding; low-density lipoprotein binding; lipid binding; high-density lipoprotein binding

Biological Process: positive regulation of blood coagulation; cGMP-mediated signaling; positive regulation of interleukin-12 production; phagocytosis, recognition; pattern recognition receptor signaling pathway; negative regulation of transcription from RNA polymerase II promoter; fatty acid metabolic process; signal transduction; negative regulation of transcription factor import into nucleus; low density lipoprotein mediated signaling; sequestering of lipid; cell surface receptor linked signal transduction; positive regulation of MAPKKK cascade; transport; regulation of transcription from RNA polymerase II promoter in response to oxidative stress; positive regulation of cell-matrix adhesion; cell adhesion; long-chain fatty acid transport; receptor-mediated endocytosis; cholesterol transport; positive regulation of I-kappaB kinase/NF-kappaB cascade; negative regulation of systemic arterial blood pressure; positive regulation of interleukin-6 production; long-chain fatty acid metabolic process; positive regulation of tumor necrosis factor production; negative regulation of angiogenesis; positive regulation of peptidyl-tyrosine phosphorylation; defense response to Gram-positive bacterium; triacylglycerol metabolic process; response to mechanical stimulus; lipoprotein transport; fatty acid oxidation; positive regulation of phagocytosis, engulfment; nitric oxide mediated signal transduction; plasma membrane long-chain fatty acid transport; apoptotic cell clearance; fatty acid transport; positive regulation of cytokine secretion

Research Articles on CD36

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Product Notes

The CD36 cd36 (Catalog #AAA217380) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's CD36 can be used in a range of immunoassay formats including, but not limited to, Flow cytometry (FC/FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blot (WB). Flow Cytometry: Use 10ul of the suggested working dilution to label 1x106 cells in 100ul. Flow Cytometry: Minimum Dilution: Neat; Maximum Dilution: 1/25. Researchers should empirically determine the suitability of the CD36 cd36 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD36, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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