Mouse anti-Human, Rat CD34 Monoclonal Antibody | anti-CD34 antibody

CD34 (Hematopoietic Progenitor Cell Antigen, HPCA1, Mucosialin) (FITC)

Cat. Num. AAA6122150

• Reactivity: Human, Rat
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence, Western Blot
• Purity: Purified by Protein A/G Affinity Chromatography.
• Synonyms: CD34; Monoclonal Antibody; CD34 (Hematopoietic Progenitor Cell Antigen; HPCA1; Mucosialin) (FITC); anti-CD34 antibody

• Host: Mouse
• Reactivity: Human, Rat
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: ICO-115
• Specificity: Recognizes human CD34. Species Crossreactivity: rat. Labels >85% of angiosarcoma and Kaposi's sarcoma, but shows low specificity.
• Purity/Purification: Purified by Protein A/G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS. No preservative added. BSA-Free. Labeled with Fluorescein isothiocyanate (FITC).

• Applicable Applications for anti-CD34 antibody: Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Blast cells of a chronic myeloid leukemia patient
• Conjugate: FITC
• Positive Control: KG-1 cells, Tonsil, or Angiosarcoma
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Flow Cytometry (FC/FACS)

(Surface flow cytometric analysis of CD34 on KG-1 cells using (red) and isotype control (green).)

Related Product Information for anti-CD34 antibody

This antibody recognizes a single chain, transmembrane, heavily glycosylated protein of 90-120kD, which is identified as CD34. Its expression is a hallmark for identifying pluripotent hematopoietic stem or progenitor cells. Its expression is gradually lost as lineage committed progenitors differentiate. CD34 is a marker of choice for staining blasts in acute myeloid leukemia. In addition, it is expressed by soft tissue tumors, such as solitary fibrous tumor and gastrointestinal stromal tumor. CD34 expression is also found in vascular endothelium. Additionally, it appears that proliferating endothelial cells overexpress this molecule than the non-proliferating endothelial cells.

Product Categories/Family for anti-CD34 antibody

Antibodies; CD Markers

References

1. Felshow DM et al. Blood 97:3768-3775 (2001). 2. Sato T et al. Blood 94:2548-2554 (1999).

NCBI and Uniprot Product Information

• NCBI GI #: 68342038
• NCBI GeneID: 947
• NCBI Accession #: NP_001020280
• NCBI GenBank Nucleotide #: NM_001025109.1
• UniProt Accession #: P28906; Q15970; Q15971; Q5JTA3; Q5JTA4; Q9UJB1; A8K664
• Molecular Weight: 35,011 Da
• NCBI Official Full Name: hematopoietic progenitor cell antigen CD34 isoform a
• NCBI Official Synonym Full Names: CD34 molecule
• NCBI Official Symbol: CD34
• NCBI Protein Information: hematopoietic progenitor cell antigen CD34; CD34 antigen
• UniProt Protein Name: Hematopoietic progenitor cell antigen CD34
• Protein Family: Hematopoietic progenitor cell antigen
• UniProt Gene Name: CD34
• UniProt Entry Name: CD34_HUMAN

Uniprot Description

CD34: Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins. Selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues. Belongs to the CD34 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Cell surface

Chromosomal Location of Human Ortholog: 1q32

Cellular Component: intercellular bridge; lysosome; integral to plasma membrane; perinuclear region of cytoplasm; cytoplasm; apical plasma membrane; basal plasma membrane; plasma membrane; external side of plasma membrane

Molecular Function: sulfate binding; carbohydrate binding; transcription factor binding

Biological Process: positive regulation of odontogenesis; regulation of immune response; negative regulation of nitric oxide biosynthetic process; glomerular filtration; cell motility involved in cell locomotion; negative regulation of blood coagulation; negative regulation of tumor necrosis factor production; signal transduction; positive regulation of interleukin-10 production; cell proliferation; cell-cell adhesion; positive regulation of angiogenesis; endothelial cell proliferation; regulation of blood pressure; tissue homeostasis; hemopoiesis; leukocyte migration