Mouse anti-Human CD33 Monoclonal Antibody | anti-CD33 antibody

Mouse Anti-Human CD33-UNLB

Cat. Num. AAA674232

• Gene Names: CD33; p67; SIGLEC3; FLJ00391; SIGLEC-3
• Reactivity: Human
• Applications: Flow Cytometry, Immunohistochemistry, Immunoprecipitation, Western Blot
• Synonyms: CD33; Monoclonal Antibody; Mouse Anti-Human CD33-UNLB; Mouse Anti-Human CD33 - Purified; Unlabeled Antibody; Mouse Anti-Human CD33; anti-CD33 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone Number: WM53
• Specificity: Human CD33; CD33 is a 67 kDa type I transmembrane glycoprotein and a member of the sialoadhesin family of cell surface receptors. It is absent from pluripotent stem cells but appears on myelomonocytic precursors after CD34. It then continues to be expressed on both the myeloid and monocyte lineages, although it is absent on granulocytes. While it has been reported that CD33 can function as a sialic acid-dependent cell adhesion molecule, cells expressing CD33 require desialylation before they can bind cells bearing the appropriate sialoglycoconjugates. This suggests that inhibitory cis interactions may regulate or block any adhesion function.1-4
• Form/Format: Purified (UNLB) Antibody
• Sequence Length: 364

• Applicable Applications for anti-CD33 antibody: Flow Cytometry; Immunohistochemistry (Frozen Sections); Immunoprecipitation; Western Blot
• Application Notes: Flow Cytometry Suggested Dilution: <= 1 ug/10^6 cells
• Characterization: To insure lot-to-lot consistency, each batch of product is tested by flow cytometry to conform with the characteristics of a standard reference reagent.
• Preparation and Storage: The purified (UNLB) antibody is supplied as 0.1 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.0. No preservatives or amine-containing buffer salts added. Store at 2- 8 degree C. ; The fluorescein (FITC) conjugate is supplied as 100 tests in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. ; The R-phycoerythrin (R-PE) and allophycocyanin (APC) conjugates are supplied as 100 tests in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze! ; The Spectral Red (SPRD) and R-phycoerythrin-Cyanine 5.5 (R-PE-CY 5.5) conjugates are supplied as 100 tests in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze! ; The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below -20 degree C. ; With the exception of the low endotoxin, azide-free product, the vials should be stored at 2-8 degree C. Conjugated forms should not be frozen and should be protected from prolonged exposure to light. Aliquot and freeze the low endotoxin, azide-free product at -20 degree C immediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored as directed.

References

1. Knapp, W., B. Dorken, W.R. GIlks, E.P. Rieber, R.E. Schmidt, H. Stein, A.E.G.K. Von dem Borne, eds. 1989. Leukocyte Typing IV: White Cell Differentiation Antigens, Oxford University Press, Oxford.
2. Schlossman, S., L. Bloumsell, W. Gilks, J.M. Harlan, C. Kishimoto, J. Ritz, S. Shaw, R. Silverstein, T. Springer, T.F. Tedder, and R.F. Todd, eds. 1995. Leukocyte Typing V: White Cell Differentiation Antigens, Oxford University Press, Oxford.
3. Barclay, A.N., M.H. Brown, S.K.A. Law, A.J. McKnight, M.G. Tomlinson, and P.A. van der Merwe, eds. 1997. The Leukocyte Antigens Facts Book, 2nd Edition, CD33 Section, Academic Press, New York, p. 213.
4. Freeman, S.D., S. Kelm, E.K. Barber, and P.R. Crocker. 1995. Blood 85:2005.

NCBI and Uniprot Product Information

• NCBI GI #: 32187777
• NCBI GeneID: 945
• UniProt Accession #: P20138; Q8TD24; Q546G0; Q8NF11
• Molecular Weight: 39,825 Da
• NCBI Official Full Name: CD33 antigen (gp67)
• NCBI Official Synonym Full Names: CD33 molecule
• NCBI Official Symbol: CD33
• NCBI Official Synonym Symbols: p67; SIGLEC3; FLJ00391; SIGLEC-3
• NCBI Protein Information: myeloid cell surface antigen CD33; gp67; CD33 antigen (gp67); sialic acid binding Ig-like lectin 3; sialic acid-binding Ig-like lectin 3
• UniProt Protein Name: Myeloid cell surface antigen CD33
• Protein Family: Myeloid cell surface antigen
• UniProt Gene Name: CD33
• UniProt Synonym Gene Names: SIGLEC3
• UniProt Entry Name: CD33_HUMAN

Uniprot Description

Function: Putative adhesion molecule of myelomonocytic-derived cells that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. Induces apoptosis in acute myeloid leukemia (in vitro). Ref.5 Ref.7

Subunit structure: Interacts with PTPN6/SHP-1 and PTPN11/SHP-2 upon phosphorylation. Ref.5 Ref.6

Subcellular location: Cell membrane; Single-pass type I membrane protein.

Tissue specificity: Monocytic/myeloid lineage cells.

Domain: Contains 2 copies of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases.

Post-translational modification: Phosphorylation of Tyr-340 is involved in binding to PTPN6 and PTPN11. Phosphorylation of Tyr-358 is involved in binding to PTPN6.

Sequence similarities: Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.Contains 1 Ig-like C2-type (immunoglobulin-like) domain.Contains 1 Ig-like V-type (immunoglobulin-like) domain.