Mouse anti-Human CD326 Monoclonal Antibody | anti-CD326 antibody
MOUSE ANTI HUMAN CD326
Gene Names
EPCAM; ESA; KSA; M4S1; MK-1; DIAR5; EGP-2; EGP40; KS1/4; MIC18; TROP1; EGP314; HNPCC8; TACSTD1
Reactivity
Human
Applications
Immunohistochemistry, Immunofluorescence, Immunohistochemistry
Synonyms
CD326; Monoclonal Antibody; MOUSE ANTI HUMAN CD326; anti-CD326 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
HEA-125
Specificity
CD326
This antibody recognises the 34kD cell surface antigen known as CD326 or Epithelial Cell Adhesion Molecule (Ep-CAM). This antigen has been identified independently by a number of groups, and it has been known by a variety of names including Epithelial Specific Antigen, MOC31 and Ber-EP4. Several monoclonal antibodies have been raised against Ep-CAM, many of which have been described as tumour specific molecules on carcinomas. Ep-CAM is a Type 1 transmembrane glycoprotein. It is expressed on the basolateral membrane of cells by the majority of epithelial tissues, with the exception of adult squamous epithelium and some specific epithelial cell types including hepatocytes and gastric epithelial cells. Ep-CAM expression has been reported to be a possible marker of early malignancy, with expression being increased in tumour cells, and de novo expression being seen in dysplastic squamous epithelium
This antibody recognises the 34kD cell surface antigen known as CD326 or Epithelial Cell Adhesion Molecule (Ep-CAM). This antigen has been identified independently by a number of groups, and it has been known by a variety of names including Epithelial Specific Antigen, MOC31 and Ber-EP4. Several monoclonal antibodies have been raised against Ep-CAM, many of which have been described as tumour specific molecules on carcinomas. Ep-CAM is a Type 1 transmembrane glycoprotein. It is expressed on the basolateral membrane of cells by the majority of epithelial tissues, with the exception of adult squamous epithelium and some specific epithelial cell types including hepatocytes and gastric epithelial cells. Ep-CAM expression has been reported to be a possible marker of early malignancy, with expression being increased in tumour cells, and de novo expression being seen in dysplastic squamous epithelium
Form/Format
Ready To Use (Purified IgG - liquid, ready to use.)
Sequence Length
314
Applicable Applications for anti-CD326 antibody
Immunohistology - Paraffin (IHC), Immunofluorescence (IF), Immunohistology - Frozen (IHC)
Application Notes
Immunohistology - Frozen: Max Dilution: Neat
Immunohistology - Paraffin: Max Dilution: Neat
Application Note: This product requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase.
Histology Positive Control Tissue: Human colon.
Immunohistology - Paraffin: Max Dilution: Neat
Application Note: This product requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase.
Histology Positive Control Tissue: Human colon.
Immunogen
HT29 carcinoma cell line
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of a mouse myeloma cell line.
Preparation
Purified IgG prepared by affinity chromatography
Preservative Stabilizers
0.09% Sodium Azide
0.5% Bovine Serum Albumin
0.5% Bovine Serum Albumin
Buffer Solution
Phosphate buffered saline
Preparation and Storage
Store at +4 degree C.
DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of dispatch.
DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of dispatch.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
34,932 Da
NCBI Official Full Name
epithelial cell adhesion molecule
NCBI Official Synonym Full Names
epithelial cell adhesion molecule
NCBI Official Symbol
EPCAM
NCBI Official Synonym Symbols
ESA; KSA; M4S1; MK-1; DIAR5; EGP-2; EGP40; KS1/4; MIC18; TROP1; EGP314; HNPCC8; TACSTD1
NCBI Protein Information
epithelial cell adhesion molecule; epithelial glycoprotein 314; human epithelial glycoprotein-2; cell surface glycoprotein Trop-1; adenocarcinoma-associated antigen; tumor-associated calcium signal transducer 1; major gastrointestinal tumor-associated protein GA733-2; membrane component, chromosome 4, surface marker (35kD glycoprotein)
UniProt Protein Name
Epithelial cell adhesion molecule
UniProt Gene Name
EPCAM
UniProt Synonym Gene Names
GA733-2; M1S2; M4S1; MIC18; TACSTD1; TROP1; Ep-CAM; EGP; EGP314; hEGP314
UniProt Entry Name
EPCAM_HUMAN
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Product Notes
The CD326 epcam (Catalog #AAA213527) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The MOUSE ANTI HUMAN CD326 reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD326 can be used in a range of immunoassay formats including, but not limited to, Immunohistology - Paraffin (IHC), Immunofluorescence (IF), Immunohistology - Frozen (IHC). Immunohistology - Frozen: Max Dilution: Neat Immunohistology - Paraffin: Max Dilution: Neat Application Note: This product requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase. Histology Positive Control Tissue: Human colon. Researchers should empirically determine the suitability of the CD326 epcam for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD326, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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