Rat anti-Mouse CD32/CD16 Monoclonal Antibody
CD32/CD16 (Fc gamma Receptor II/III, FcRII/III) (PE)
Reactivity
Mouse
Applications
Flow Cytometry, Functional Assay
Purity
Affinity PurifiedPurified by Protein G affinity chromatography.
Synonyms
CD32/CD16; Monoclonal Antibody; CD32/CD16 (Fc gamma Receptor II/III; FcRII/III) (PE); Anti -CD32/CD16 (Fc gamma Receptor II/III; anti-CD32/CD16 antibody
Host
Rat
Reactivity
Mouse
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
6A284
Specificity
Recognizes an epitope expressed by the murine low affinity Fc receptors, Fc gamma III (CD16) and Fc gamma II (CD32). In the mouse only the transmembrane form of CD16 is reported to exist, which is expressed on macrophages, NK cells, granulocytes, myeloid precursors, and subpopulations of T lymphocytes. CD32 is primarily expressed on cells of the myeloid lineage and also on mature B lymphocytes.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Purified by Protein G affinity chromatography.
Form/Format
Supplied as a lyphilized powder in PBS, pH 7.4, 1% BSA, 0.09% sodium azide.
Applicable Applications for anti-CD32/CD16 antibody
Flow Cytometry (FC/FACS)
Application Notes
Suitable for use in Flow Cytometry.
Dilution: Flow Cytometry:Neat, 1:10 Use 10ul to label 10e6 cells in 100ul.
Optimal dilution determined by the researcher
Dilution: Flow Cytometry:Neat, 1:10 Use 10ul to label 10e6 cells in 100ul.
Optimal dilution determined by the researcher
Immunogen
PU5 1.8 IOE7 Balb/c mouse cell line.
Recommended Negative Controls
Rat IgG2b
Hybridoma
Cells from immunized rats were fused with cells of the mouse P3X63-Ag8.653 myeloma cell line.
Preparation and Storage
Lyophilized powder may be stored at 4 degree C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Product Categories/Family for anti-CD32/CD16 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
CD32
Similar Products
Product Notes
The CD32/CD16 (Catalog #AAA601838) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The CD32/CD16 (Fc gamma Receptor II/III, FcRII/III) (PE) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CD32/CD16 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS). Suitable for use in Flow Cytometry. Dilution: Flow Cytometry:Neat, 1:10 Use 10ul to label 10e6 cells in 100ul. Optimal dilution determined by the researcher. Researchers should empirically determine the suitability of the CD32/CD16 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD32/CD16, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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