Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using MBS767691 (CD3E Antibody) at dilution of 1:8000)
Mouse anti-Human CD3 epsilon Monoclonal Antibody | anti-CD3 epsilon antibody
CD3 epsilon Mouse Monoclonal
Reactivity
Human
Applications
ELISA, Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry, Functional Assay
Purity
Purification: Protein A+G purificationPurity: >95% as determined by SDS-PAGE
Synonyms
CD3 epsilon; Monoclonal Antibody; CD3 epsilon Mouse Monoclonal; CD3; CD3E; CD3-epsilon; T3E; TCRE; anti-CD3 epsilon antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Specificity
other species are not tested. Please decide the specificity by homology
Purity/Purification
Purification: Protein A+G purification
Purity: >95% as determined by SDS-PAGE
Purity: >95% as determined by SDS-PAGE
Form/Format
Liquid; PBS with 0.02% sodium azide and 50% glycerol pH 7.3
Sequence Length
202
Applicable Applications for anti-CD3 epsilon antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:2000 - 1:20000
IHC: 1:1000 - 1:10000
IF: 1:50 - 500
IHC: 1:1000 - 1:10000
IF: 1:50 - 500
Immunogen
CD3e molecule, epsilon (CD3-TCR complex)
Preparation and Storage
-20 degree C for 24 months (Avoid repeated freeze / thaw cycles.)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using MBS767691 (CD3E Antibody) at dilution of 1:8000)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using MBS767691 (CD3E Antibody) at dilution of 1:8000)
Western Blot (WB)
(Jurkat cells were subjected to SDS PAGE followed by western blot with MBS767691 (CD3E Antibody) at dilution of 1:10000)
Western Blot (WB)
(Jurkat cells were subjected to SDS PAGE followed by western blot with MBS767691 (CD3E Antibody) at dilution of 1:10000)
Related Product Information for anti-CD3 epsilon antibody
CD3 is a complex of proteins that directly associates with the T cell receptor (TCR). The TCR/CD3 complex of T-lymphocytes consists of either a TCR alpha/beta or TCR gamma/delta heterodimer coexpressed at the cell surface with the invariant subunits of CD3 labeled gamma, delta, epsilon, zeta, and eta. The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction. CD3 is considered to be a pan-T cell marker for detection of normal and neoplastic T cells. This anti-CD3 is a monoclonal antibody directed against the epsilon chain of human CD3 molecule.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
23 kDa
NCBI Official Full Name
CD3 epsilon
NCBI Official Symbol
CD3E
NCBI Protein Information
T-cell surface glycoprotein CD3 epsilon chain
UniProt Protein Name
T-cell surface glycoprotein CD3 epsilon chain
UniProt Gene Name
CD3E
UniProt Entry Name
CD3E_CANLF
Uniprot Description
The CD3 complex mediates signal transduction, resulting in T-cell activation and proliferation. Required for normal immune responses.
Research Articles on CD3 epsilon
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Product Notes
The CD3 epsilon cd3e (Catalog #AAA767691) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The CD3 epsilon Mouse Monoclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD3 epsilon can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FC/FACS). WB: 1:2000 - 1:20000 IHC: 1:1000 - 1:10000 IF: 1:50 - 500. Researchers should empirically determine the suitability of the CD3 epsilon cd3e for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD3 epsilon, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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