Mouse anti-Human CD3 Monoclonal Antibody | anti-CD3 antibody

CD3 (MaxLight 750)

Cat. Num. AAA6256648

• Gene Names: CD3D; T3D; IMD19; CD3-DELTA
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunoprecipitation
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: CD3; Monoclonal Antibody; CD3 (MaxLight 750); anti-CD3 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone Number: MEM-57
• Specificity: Recognizes human CD3. Species Crossreactivity: Chimpanzee
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight750.

• Applicable Applications for anti-CD3 antibody: Flow Cytometry (FC/FACS), Immunoprecipitation (IP)
• Conjugate: MaxLight750
• Preparation and Storage: Store at 4 degree C. Do Not Freeze.

Testing Data

(Staining of human peripheral blood lymphocytes with Mouse anti Human CD3:FITC)

Testing Data

(Staining of human peripheral blood lymphocytes with Mouse anti Human CD3:RPE)

Product Categories/Family for anti-CD3 antibody

Antibodies; CD Markers

References

1. Horejsi, V. et al. (1998) Monoclonal antibodies against human leucocyte antigens II. Antibodies against CD45 (T200), CD3 (T3), CD43, CD10 (CALLA) transferrin receptor (T9), a novel broadly expressed 18kDa antigen (MEM-43) and a novel antigen of restricted expression (MEM-74). Folia. Biol. (Praha). 34: 23-34. 2. Dave, V.P. et al. (1998) Altered functional responsiveness of thymocyte subsets from CD3delta- deficient mice to TCR-CD3 engagement. Int Immunol. 10: 1481-90. 3. Transy, C. et al. (1989) Most anti-human CD3 monoclonal antibodies are directed to the CD3 epsilon subunit. Eur J Immunol. 19: 947-50. 4. Amlot, P.L. et al. (1996) Activation antigen expression on human T cells. I. Analysis by two-color flow cytometry of umbilical cord blood, adult blood and lymphoid tissue. Clin Exp Immunol. 105: 176-82. 5. Gunnlaugsdottir, B. et al. (2008) Naive human T-cells become non-responsive towards anti-TNFalpha (infliximab) treatment in vitro if co-stimulated through CD28. Scand J Immunol. 68: 624-34. 6. Morris, R.J. et al. (2005) A high-efficiency system of natural killer cell cloning. J Immunol Methods. 307: 24-33.

NCBI and Uniprot Product Information

• NCBI GI #: 115985
• NCBI GeneID: 915
• UniProt Accession #: P04234; A8MVP6
• Molecular Weight: 171
• NCBI Official Full Name: T-cell surface glycoprotein CD3 delta chain
• NCBI Official Synonym Full Names: CD3d molecule, delta (CD3-TCR complex)
• NCBI Official Symbol: CD3D
• NCBI Official Synonym Symbols: T3D; IMD19; CD3-DELTA
• NCBI Protein Information: T-cell surface glycoprotein CD3 delta chain; OKT3, delta chain; CD3 antigen, delta subunit; T-cell receptor T3 delta chain; CD3d antigen, delta polypeptide (TiT3 complex)
• UniProt Protein Name: T-cell surface glycoprotein CD3 delta chain
• Protein Family: CD302 antigen
• UniProt Gene Name: CD3D
• UniProt Synonym Gene Names: T3D
• UniProt Entry Name: CD3D_HUMAN

NCBI Description

The protein encoded by this gene is part of the T-cell receptor/CD3 complex (TCR/CD3 complex) and is involved in T-cell development and signal transduction. The encoded membrane protein represents the delta subunit of the CD3 complex, and along with four other CD3 subunits, binds either TCR alpha/beta or TCR gamma/delta to form the TCR/CD3 complex on the surface of T-cells. Defects in this gene are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (SCIDBNK). Two transcript variants encoding different isoforms have been found for this gene. Other variants may also exist, but the full-length natures of their transcripts has yet to be defined. [provided by RefSeq, Feb 2009]

Uniprot Description

CD3D: a T cell surface glycoprotein that is a component of the T cell antigen receptor. A defect in this protein causes a severe combined immunodeficiency characterized by the absence of T cells but normal B cells. Contains 1 ITAM domain.

Protein type: Membrane protein, integral; Receptor, misc.

Chromosomal Location of Human Ortholog: 11q23

Cellular Component: T cell receptor complex; cytoplasm; plasma membrane; integral to membrane; alpha-beta T cell receptor complex

Molecular Function: transmembrane receptor activity; protein heterodimerization activity; transcription coactivator activity

Biological Process: regulation of immune response; cell surface receptor linked signal transduction; T cell costimulation; positive regulation of transcription from RNA polymerase II promoter; T cell receptor signaling pathway; T cell differentiation; positive thymic T cell selection

Disease: Immunodeficiency 19