Mouse anti-Rat CD3 Monoclonal Antibody | anti-CD3 antibody
CD3 mouse mAb (PE)
Reactivity
Rat
Applications
Flow Cytometry, Functional Assay
Purity
Affinity purified
Synonyms
CD3; Monoclonal Antibody; CD3 mouse mAb (PE); anti-CD3 antibody
Host
Mouse
Reactivity
Rat
Clonality
Monoclonal
Isotype
IgM, kappa
Purity/Purification
Affinity purified
Form/Format
Aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Applicable Applications for anti-CD3 antibody
Flow Cytometry (FC/FACS)
Immunogen
F344 rat spleen cells stimulated with PMA and calcium ionophore
Conjugation
PE
Modification
Unmodified
Notes
The following is a general protocol, optimization is required for best results of your experiment.)
1. Take 100 ul peripheral blood anticoagulated by EDTA and add to the bottom of 5 ml tube
2. Add <=1.0ug labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light
3. Add 2 ml 1×RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells
4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant
5. Add 2 ml PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant
6. Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be tested on the day on the machine, or be added fixation overnight at 4 degree C then measured); [PBS wash buffer: PBS +1% FBS +0.1% NaN3] [Cell fixation: 2% formaldehyde solution]
1. Take 100 ul peripheral blood anticoagulated by EDTA and add to the bottom of 5 ml tube
2. Add <=1.0ug labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light
3. Add 2 ml 1×RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells
4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant
5. Add 2 ml PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant
6. Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be tested on the day on the machine, or be added fixation overnight at 4 degree C then measured); [PBS wash buffer: PBS +1% FBS +0.1% NaN3] [Cell fixation: 2% formaldehyde solution]
Preparation and Storage
Store at 4 degree C. DO NOT freeze. Light sensitive.
Related Product Information for anti-CD3 antibody
CD3 is a complex composed of delta, gamma, epsilon, and zeta chains. They are 20-25 kD members of the immunoglobulin superfamily and associated with the T cell receptor (TCR).CD3 is expressed on thymocytes, peripheral T cells, some NK-T cells, and dendritic epidermal T cells. CD3 is involved in antigen recognition, signal transduction, and T cell activation.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
21312 MW
NCBI Official Full Name
T-cell surface glycoprotein CD3 epsilon chain
NCBI Official Synonym Full Names
CD3e molecule
NCBI Official Symbol
Cd3e
NCBI Protein Information
T-cell surface glycoprotein CD3 epsilon chain
UniProt Protein Name
CD3 antigen, epsilon polypeptide (Predicted)
Protein Family
UniProt Gene Name
Cd3e
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Product Notes
The CD3 cd3e (Catalog #AAA4753698) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The CD3 mouse mAb (PE) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's CD3 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS). Researchers should empirically determine the suitability of the CD3 cd3e for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD3, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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