Mouse anti-Human CD3 Monoclonal Antibody | anti-CD3 antibody

Mouse anti CD3 (T3)-Hu Monoclonal Antibody

Cat. Num. AAA460440

• Gene Names: CD247; T3Z; CD3H; CD3Q; CD3Z; TCRZ; IMD25; CD3-ZETA
• Reactivity: Human
• Applications: ELISA, Flow Cytometry, Functional Assay, Immunohistochemistry, Immunoprecipitation, Western Blot
• Purity: Purified by Protein A-Affinity Chromatography according to Isotyping
• Synonyms: CD3; Monoclonal Antibody; Mouse anti CD3 (T3)-Hu Monoclonal Antibody; anti-CD3 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Specificity: This antibody recognizes human CD3 protein. The other species was not tested.
• Purity/Purification: Purified by Protein A-Affinity Chromatography according to Isotyping
• Form/Format: Supplied in sterile Phosphate buffered saline (pH7.2) containing antibody stabilizer.
• Sequence Length: 167

• Applicable Applications for anti-CD3 antibody: ELISA (EIA), Flow Cytometry (FC/FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB)
• Application Notes: ELISA: 0.01-0.1 ug/ml; Flow Cytometry: 5-10 ug/ml; Immunohistochemistry: 2-10 ug/ml; Immunoprecipitation: 2-5 ug/ml; Western Blot: 0.1-1 ug/ml
• Preparation: Human T cells expressed CD3
• Preparation and Storage: The antibodies are stable for 12 months from date of receipt when stored at -20 degree C. The antibodies can be stored at 2 degree C - 8 degree C for one month without detectable loss of activity. Avoid repeated freezing-thawing cycles.

Related Product Information for anti-CD3 antibody

CD3 is a glycoprotein consisting of up to five polypeptide chains (gp20, gp26, p16, p20, p28). CD3 is required for proper assembly, trafficking and surface expression of TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. CD3 is involved in T cell activation, proliferation and apoptosis pathway. This protein is present on virtually all peripheral blood T cells and thymocytes. This anti-CD3 antibody is commonly used as a phenotypic marker for human T cells.

References

Kearse KP. Calnexin associates with monomeric and oligomeric (disulfidelinked) CD3delta proteins in murine T lymphocytes. J Biol Chem. 1998 Jun 5;273 (23):141527.

NCBI and Uniprot Product Information

• NCBI GI #: 1914807
• NCBI GeneID: 919
• UniProt Accession #: P79951; O13176; P20963; A46461
• Molecular Weight: 18,897 Da
• NCBI Official Full Name: cd3
• NCBI Official Synonym Full Names: CD247 molecule
• NCBI Official Symbol: CD247
• NCBI Official Synonym Symbols: T3Z; CD3H; CD3Q; CD3Z; TCRZ; IMD25; CD3-ZETA
• NCBI Protein Information: T-cell surface glycoprotein CD3 zeta chain
• UniProt Protein Name: CD3gamma/delta
• Protein Family: CD302 antigen
• UniProt Gene Name: cd3g

NCBI Description

The protein encoded by this gene is T-cell receptor zeta, which together with T-cell receptor alpha/beta and gamma/delta heterodimers, and with CD3-gamma, -delta and -epsilon, forms the T-cell receptor-CD3 complex. The zeta chain plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. Low expression of the antigen results in impaired immune response. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]