Mouse anti-Human CD28 Monoclonal Antibody | anti-CD28 antibody

Human CD28 (3G5E) Monoclonal Antibody

Cat. Num. AAA3014184

• Gene Names: CD28; Tp44
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, Immunoprecipitation
• Purity: Protein A purified mouse monoclonal antibody, >95% immunoglobulin by SDS-PAGE
• Synonyms: CD28; Monoclonal Antibody; Human CD28 (3G5E) Monoclonal Antibody; T-cell-specific surface glycoprotein CD28; TP44; anti-CD28 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Specificity: Recognizes the CD28 protein.
• Purity/Purification: Protein A purified mouse monoclonal antibody, >95% immunoglobulin by SDS-PAGE
• Form/Format: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 123

• Applicable Applications for anti-CD28 antibody: Flow Cytometry (FC/FACS), Immunohistochemistry (IHC) Frozen, Immunoprecipitation (IP), T Cell Co-stimulation
• Application Notes: FC/FACS: 5 ug/ml; IHC-Fr: User-optimized; IP: User-optimized; T cell Co-stimulation: User-optimized
• Immunogen: Human CD28 Ig fusion protein and HPB-MLT human tumor cells.
• Preparation and Storage: Following initial use, aliquot and freeze (-20 degree C) for long-term storage.; Avoid freeze/thaw cycles.

Related Product Information for anti-CD28 antibody

Human CD28 is an important co-stimulatory molecule found on all CD4+ T cells and ~50% of CD8+ T cells. T cell activities attributed to CD28 include prevention of anergy, induction of cytokine gene transcription, stabilization of cytokine mRNA, and activation of CD8+ cytotoxic T lymphocytes. The ligands for CD28, known as CD80 (B7-1) and CD86 (B7-2), are monomeric transmembrane glycoproteins.

NCBI and Uniprot Product Information

• NCBI GI #: 340545508
• NCBI GeneID: 940
• NCBI Accession #: NP_001230006.1
• NCBI GenBank Nucleotide #: NP_001230006.1
• UniProt Accession #: P10747
• Molecular Weight: ~ 25kDa
• NCBI Official Full Name: T-cell-specific surface glycoprotein CD28 isoform 2
• NCBI Official Synonym Full Names: CD28 molecule
• NCBI Official Symbol: CD28
• NCBI Official Synonym Symbols: Tp44
• NCBI Protein Information: T-cell-specific surface glycoprotein CD28
• UniProt Protein Name: T-cell-specific surface glycoprotein CD28
• Protein Family: T-cell-specific surface glycoprotein
• UniProt Gene Name: CD28
• UniProt Entry Name: CD28_HUMAN

NCBI Description

The protein encoded by this gene is essential for T-cell proliferation and survival, cytokine production, and T-helper type-2 development. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jul 2011]

Uniprot Description

CD28: a type I plasma membrane protein Involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival. Binds to B7-1 and -2. Expressed in T cells and plasma cells, but not in less mature B cells. Six splice-variant isoforms have been described.

Protein type: Cell surface; Membrane protein, integral

Chromosomal Location of Human Ortholog: 2q33

Cellular Component: integral to plasma membrane; plasma membrane; immunological synapse; cytosol; external side of plasma membrane

Molecular Function: identical protein binding; protein binding; SH3/SH2 adaptor activity; protease binding; coreceptor activity

Biological Process: positive regulation of isotype switching to IgG isotypes; positive regulation of translation; nerve growth factor receptor signaling pathway; viral reproduction; positive regulation of interleukin-2 biosynthetic process; cytokine biosynthetic process; T cell receptor signaling pathway; positive regulation of inflammatory response to antigenic stimulus; cell surface receptor linked signal transduction; positive regulation of T cell proliferation; regulatory T cell differentiation; epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; positive regulation of viral genome replication; positive regulation of mitosis; negative thymic T cell selection; regulation of defense response to virus by virus; humoral immune response; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein kinase B signaling cascade; T cell costimulation; innate immune response; positive regulation of alpha-beta T cell proliferation; positive regulation of transcription from RNA polymerase II promoter