anti-Mouse CD28 Monoclonal Antibody | anti-Cd28 antibody

Hamster Anti-Mouse CD28-LE/AF

Cat. Num. AAA673304

• Gene Names: Cd28; Cd28
• Reactivity: Mouse
• Applications: Flow Cytometry, Immunoprecipitation
• Synonyms: CD28; Monoclonal Antibody; Hamster Anti-Mouse CD28-LE/AF; Hamster Anti-Mouse CD28 - Low Endotoxin/Azide-Free; Hamster Anti-Mouse CD28; anti-Cd28 antibody

• Host: Syrian Hamster
• Reactivity: Mouse
• Clonality: Monoclonal
• Isotype: Syrian Hamster IgG lambda
• Clone Number: 37.51
• Specificity: CD28 costimulatory receptor, Mr 40 kDa; CD28 is a type I disulfide-linked homodimer that is constitutively expressed on most thymocytes, at low density on nearly all CD4^+ and CD8^+ peripheral T lymphocytes, and at very low levels on NK cells. Its expression is upregulated upon T-cell activation.^1-4 CD28 is a ligand for CD80/B7-1 and CD86/B7-2 on B cells and other antigen presenting cells, and plays an important role in the interaction between T cells and B cells. CD28 is a co-stimulatory receptor involved in many, but not all, T-cell independent immune responses.^5-8
• Form/Format: Low Endotoxin, Azide - Free (LE/AF)
• Sequence Length: 63

• Applicable Applications for anti-Cd28 antibody: Flow Cytometry; Immunoprecipitation
• Application Notes: Research Application(s): In vitro costimulation of T cells and NK cells
• Characterization: To ensure lot-to-lot consistency, each batch of monoclonal antibody is tested by flow cytometry to conform to characteristics of a standard reference reagent. Representative data are included in this product insert.
• Preparation and Storage: The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2-8 degree C. ; The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C. ; The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C. ; The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL or 0.2 mg in 2.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze! ; The allophycocyanin (APC) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing ; The Spectral Red (SPRD) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze! ; The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below -20 degree C. ; Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide-free product at -20 degree C immediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored as directed.

References

1. Gross, J. A., E. Callas, and J. P. Allison. 1992. J. Immunol. 149:380.
2. Giese, T., J. P. Allison, and W. F. Davidson. 1993. J. Immunol. 151:597.
3. Clements, J. L., G. Winslow, C. Donahue, S. M. Cooper, J. P. Allison, and R. C. Budd. 1993. Int. Immunol. 5:1451.
4. Nandi, D., J. A. Gross, and J. P. Allison. 1994. J. Immunol. 152:3361.
5. June, C.H., J.A. Bluestone, L.M. Nadler, and C.B. Thompson. 1992. Immunol. Today 15:321.
6. Gelfanov, V., Y. G. Lai, V. Gelfanova, J. Y. Dong, J. P. Su, and N. S. Liao. 1995. J. Immunol. 155:76.
7. Harding, F. A., J. G. McArthur, J. A. Gross, D. H. Raulet, and J. P. Allison. 1992. Nature 356:607.
8. Bluestone, J.A. 1995. Immunity 2:555.

NCBI and Uniprot Product Information

• NCBI GI #: 192485
• NCBI GeneID: 12487
• UniProt Accession #: P31041; Q8CDB3
• NCBI Official Full Name: CD28
• NCBI Official Synonym Full Names: CD28 antigen
• NCBI Official Symbol: Cd28
• NCBI Official Synonym Symbols: Cd28
• NCBI Protein Information: T-cell-specific surface glycoprotein CD28; OTTMUSP00000002157
• UniProt Protein Name: T-cell-specific surface glycoprotein CD28
• Protein Family: T-cell-specific surface glycoprotein
• UniProt Entry Name: CD28_MOUSE

Uniprot Description

CD28: a type I plasma membrane protein Involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival. Binds to B7-1 and -2. Expressed in T cells and plasma cells, but not in less mature B cells. Six splice-variant isoforms have been described.

Protein type: Membrane protein, integral; Cell surface

Cellular Component: membrane; plasma membrane; integral to membrane; immunological synapse; lipid raft; external side of plasma membrane

Molecular Function: protein homodimerization activity; SH3/SH2 adaptor activity; protease binding

Biological Process: positive regulation of isotype switching to IgG isotypes; positive regulation of mitosis; T cell activation; positive regulation of interleukin-2 biosynthetic process; T cell receptor signaling pathway; negative thymic T cell selection; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein kinase B signaling cascade; positive regulation of inflammatory response to antigenic stimulus; T cell costimulation; positive regulation of transcription from RNA polymerase II promoter; immune response; positive regulation of alpha-beta T cell proliferation; positive regulation of T cell proliferation; regulatory T cell differentiation