Mouse anti-Human CD257 Monoclonal Antibody | anti-CD257 antibody

anti-human CD257(BAFF)-Purified Preservative Free

Cat. Num. AAA666726

• Reactivity: Human
• Applications: ELISA
• Purity: Purified Preservative Free
• Synonyms: CD257; Monoclonal Antibody; anti-human CD257(BAFF)-Purified Preservative Free; Monoclonal anti-human CD257 (BAFF; Tall-1); anti-CD257 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1k
• Clone Number: ANC2H3
• Purity/Purification: Purified Preservative Free
• Form/Format: 50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl
• Concentration: 1.0 mg/ml (varies by lot)

• Applicable Applications for anti-CD257 antibody: ELISA (EIA)
• Performance: Activity of anti-CD257 antibody was determined by its ability to block binding of recombinant CD257 to receptors present on Raji cell surface. Five x 105 human Raji cells were washed and incubated 45 minutes on ice with 80 ul of recombinant CD257(BAFF)trn-muCD8 at 0.5 ug/ml. Cells were washed twice and incubated with 2 degree anti-muCD8a/PE, after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.07 log10 fluorescent units when compared to a buffer control. Binding was 87% inhibited when reagent was pre incubated with 5 ug/ml anti-CD257 antibody.
• Production: Antibody was Protein A purified from (low FBS containing) tissue culture supernatant. Purity was >95% Immunoglobulin by SDS-PAGE and contains less than 1% Bovine Immunoglobulin. Product was 0.2u sterile filtered and vialed under aseptic conditions.
• Buffer: 50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl.
• Immunogen: Recombinant soluble human CD257(BAFF)
• Preparation and Storage: Store at 2 - 5 degree C. Freeze/Thawing is not recommended. Open under aseptic conditions.; Product should retain activity for at least 12 months after shipping date when stored as recommended.

Related Product Information for anti-CD257 antibody

Information: Human CD257 (B cell activating factor, BAFF,TALL-1, Blys, THANK) and CD256 (APRIL, a proliferation inducing ligand) are both type II molecules belonging to the TNF superfamily (TNFSFL #13b and 13 respectively). They are expressed by non-B cells, and are down regulated by mitogenic stimulation(2). BAFF and APRIL bind to at least two receptors: CD267 (TACI, transmembrane activator and CAML-interactor) and CD269 (BCMA, B cell maturation antigen), both of which are restricted to B cells(3,4). Ligation of these receptors with recombinant BAFF dramatically increases IgM production by peripheral blood B cells(1). A third receptor CD268 (BAFFR) is specific for BAFF has also been described(5). BAFF and BAFFR knockout mice have a reduced numbers of mature B cells in the periphery, however TACI and BCMA knockouts do not share this phenotype, suggesting that BAFF-R may the primary receptor for BAFF in mice(8,9,10). Cell surface BAFF can be proteolytically cleaved to form a soluble trimeric molecule(2). Levels of soluble BAFF correspond with levels of autoantibodies in Sjogren's Syndrome(11). Clone ANC2H3 blocks binding of recombinant human CD257(BAFF) to receptors on Raji cells in Flow Cytometry.

References

1) Schneider P., J. Tschopp, et al. J. Exp. Med. 1999, 189(11):1747-1756. 2) Shu, H.B., H. Johnson, W.H. Hui. J Leukoc Biol 1999, 65:680-683. 3) Marsters, S.A., A. Ashkenazi, et al. 2000, Curr Biol 10:785-788. 4) Xia, X., H. Hsu, et al. 2000, J Exp Med, 192(1): 137-143. 5) Thompson J.S., C. Ambrose, et al. Science 2001, 293: 2108-2111. 6) Roschke, V, T.S. Migone, et al. J Immunol. 2002, 169: 4314-4321. 7) MacLennan, C.M., C.G. Vinuesa, 2002, Immunity 17:235-238. 8) B. Schiemann, et al, (2001) Science 293: 2111-2114. 9) S.M. Harless,et al, (2001) Curr Biol 11: 1988-1989. 10) Mol Cell Biol (2001) 21: 4067-4074. 11) X. Mariette, et al, (2003) Ann Rhem Dis 62: 168-171.