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Rat CD25 Monoclonal Antibody | anti-CD25 antibody

RAT ANTI MOUSE CD25:RPE

Gene Names
Il2ra; CD25; Il2r; Ly-43
Applications
Flow Cytometry, Functional Assay
Synonyms
CD25; Monoclonal Antibody; RAT ANTI MOUSE CD25:RPE; anti-CD25 antibody
Ordering
For Research Use Only!
Host
Rat
Clonality
Monoclonal
Isotype
IgG1
Clone Number
PC61.5.3
Form/Format
Purified IgG conjugated to R. Phycoerythrin (RPE) - liquid
Concentration
IgG concentration 0.1 mg/ml (varies by lot)
Sequence Length
268
Applicable Applications for anti-CD25 antibody
Flow cytometry (FC/FACS)
Application Notes
Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity fc receptors. This may be reduced by using SeroBlock FcR.
Flow Cytometry: Maximum Dilution: Neat
Perservative Stabilisers
0.02% Sodium Azide
0.5% Bovine Serum Albumin
Preparation
Immunogen
B6.1 CTL cell line.
Fusion Partners
Target Species
Mouse
Preparation and Storage
Store at 4 degree C. DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 6 months from date of despatch.
Related Product Information for anti-CD25 antibody
Rat anti Mouse CD25 antibody, clone PC61.5.3 reacts with the low affinity alpha chain of the interleukin-2 receptor present on activated T and B cells in mice. Rat anti Mouse CD25 antibody, clone PC61.5.3 is reported to inhibit IL-2 binding and IL-2 dependent proliferation.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
30,723 Da
NCBI Official Full Name
interleukin-2 receptor subunit alpha
NCBI Official Synonym Full Names
interleukin 2 receptor, alpha chain
NCBI Official Symbol
Il2ra
NCBI Official Synonym Symbols
CD25; Il2r; Ly-43
NCBI Protein Information
interleukin-2 receptor subunit alpha; IL-2 receptor subunit alpha; IL-2-RA; IL-2R alpha chain; IL-2R subunit alpha; IL2-RA; p55 chain
UniProt Protein Name
Interleukin-2 receptor subunit alpha
UniProt Gene Name
Il2ra
UniProt Synonym Gene Names
Il2r; IL-2 receptor subunit alpha; IL-2-RA; IL-2R subunit alpha; IL2-RA
UniProt Entry Name
IL2RA_MOUSE

Uniprot Description

IL2RA: Receptor for interleukin-2. Non-covalent dimer of an alpha and a beta subunit. IL2R exists in 3 different forms: a high affinity dimer, an intermediate affinity monomer (beta subunit), and a low affinity monomer (alpha subunit). The high and intermediate affinity forms also associate with a gamma subunit.

Protein type: Membrane protein, integral

Cellular Component: cell surface; membrane; integral to membrane; external side of plasma membrane

Molecular Function: protein binding; interleukin-2 receptor activity; drug binding; interleukin-2 binding

Biological Process: Notch signaling pathway; regulation of T cell homeostatic proliferation; negative regulation of immune response; negative regulation of defense response to virus; positive regulation of activated T cell proliferation; negative regulation of lymphocyte proliferation; negative regulation of T cell proliferation; inflammatory response to antigenic stimulus; positive regulation of T cell differentiation; negative regulation of inflammatory response; positive regulation of T cell proliferation; T cell homeostasis; immune response; activated T cell apoptosis

Research Articles on CD25

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Product Notes

The CD25 il2ra (Catalog #AAA213567) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's CD25 can be used in a range of immunoassay formats including, but not limited to, Flow cytometry (FC/FACS). Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity fc receptors. This may be reduced by using SeroBlock FcR. Flow Cytometry: Maximum Dilution: Neat. Researchers should empirically determine the suitability of the CD25 il2ra for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD25, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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