Rat anti-Mouse CD24/HSA Monoclonal Antibody
Rat Anti-Mouse CD24/HSA-FITC
Reactivity
Mouse
Applications
Flow Cytometry, Immunoprecipitation
Synonyms
CD24/HSA; Monoclonal Antibody; Rat Anti-Mouse CD24/HSA-FITC; Rat Anti-Mouse CD24/HSA - FITC; Rat Anti-Mouse CD24/HSA; anti-CD24/HSA antibody
Host
Rat
Reactivity
Mouse
Clonality
Monoclonal
Isotype
Rat IgG2c kappa
Clone Number
30-F1
Specificity
Mouse CD24/heat stable antigen (HSA)
CD24 is expressed in B cells until their terminal differentiation into plasma cells.1 It has been used widely as a marker to fractionate different B-lineage differentiation stages.10 CD24 has been found on a variety of other hematopoietic and neural cell types, and is now classified as a costimulatory molecule.2-4 The CD24/HSA molecule is composed mostly of carbohydrate, with a protein core of only 31-35 amino acids in the human and 27 amino acids in the mouse.1, 3 The molecular mass is variable, ranging from 38-70 kDa.3 CD24 is now included in the large group of phosphatidylinositol-anchored membrane proteins.1-4 Studies with purified CD24 applied to latex beads have demonstrated a self-binding property, suggesting that the molecule is capable of promoting homotypic aggregation, binding and cell adhesion.3 This is supported by earlier observations of the blockade of B cell lymphoblast aggregation with anti- CD24 monoclonal antibody.6 Recent studies of murine CD24 have assigned a role for CD24/HSA as a ligand for P-selectin, a calcium-dependent lectin.7
CD24 is expressed in B cells until their terminal differentiation into plasma cells.1 It has been used widely as a marker to fractionate different B-lineage differentiation stages.10 CD24 has been found on a variety of other hematopoietic and neural cell types, and is now classified as a costimulatory molecule.2-4 The CD24/HSA molecule is composed mostly of carbohydrate, with a protein core of only 31-35 amino acids in the human and 27 amino acids in the mouse.1, 3 The molecular mass is variable, ranging from 38-70 kDa.3 CD24 is now included in the large group of phosphatidylinositol-anchored membrane proteins.1-4 Studies with purified CD24 applied to latex beads have demonstrated a self-binding property, suggesting that the molecule is capable of promoting homotypic aggregation, binding and cell adhesion.3 This is supported by earlier observations of the blockade of B cell lymphoblast aggregation with anti- CD24 monoclonal antibody.6 Recent studies of murine CD24 have assigned a role for CD24/HSA as a ligand for P-selectin, a calcium-dependent lectin.7
Form/Format
Fluorescein (FITC) Conjugate
Applicable Applications for anti-CD24/HSA antibody
Flow Cytometry; Immunoprecipitation
Application Notes
Flow Cytometry Suggested Dilution: <= 2 ug/10^6 cells
Characterization
To ensure lot-to-lot consistency, each batch of monoclonal antibody is tested by flow cytometry to conform to characteristics of a standard reference reagent. Representative data are included in this product insert.
Preparation and Storage
The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2-8 degree C.
The fluorescein (FITC) conjugate is supplied as 0.5 mg or 0.1 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 degree C.
The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The R-phycoerythrin (R-PE) and allophycocyanin (APC) conjugates are supplied as 0.1 mg in 1.0 mL or 0.2 mg in 2.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The Spectral Red (SPRD) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below -20 degree C.
Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide-free product at -20 degree C immediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored as directed.
The fluorescein (FITC) conjugate is supplied as 0.5 mg or 0.1 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 degree C.
The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The R-phycoerythrin (R-PE) and allophycocyanin (APC) conjugates are supplied as 0.1 mg in 1.0 mL or 0.2 mg in 2.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The Spectral Red (SPRD) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below -20 degree C.
Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide-free product at -20 degree C immediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored as directed.
References
1. Kay, R., P. M. Rosten and R. K. Humphries. 1991. J. Immunol. 147:1412-1416.
2. Fischer, G. F., O. Majdic, S. Gadd, and W. Knapp. 1990. J. Immunol. 144:638.
3. Hubbe, M. and P. Altevogt. 1994. Eur. J. Immunol. 1994. 24:731.
4. Liu, Y., B. Jones, W. Brady, J. C. A. Janeway, and P. S. Linsley. 1992. Eur. J. Immunol. 22:2855.
5. Shields, J. G., S. H. Smith and R. E. Callard. 1987. In Leukocyte Typing III. A. J. McMichael, et. al., eds., Oxford University Press, Oxford. P. 488.
6. Kadmon, G., M. Eckert, M. Sammar, M. Schachner, and P. Altevogt. 1992. J. Cell Biol. 118:1245.
7. Aigner, S., M. Ruppert, M. Hubbe, M. Sammar, Z. Sthoeger, E. C. Butcher, D. Vestweber, and P. Altevogt. 1995. International Immunology 7:1557.
8. Ledbetter, J. A., and L. A. Herzenberg. 1979. Immunol. Rev. 47: 63.
9. Kariv, I., R. R. Hardy and K. Hayakawa. 1994. J. Exp. Med. 180:2419.
10. Hardy, R. R., C. E. Carmack, S. A. Shinton, J. D. Kemp, and K. Hayakawa. 1991. J. Exp. Med 173:1213.
2. Fischer, G. F., O. Majdic, S. Gadd, and W. Knapp. 1990. J. Immunol. 144:638.
3. Hubbe, M. and P. Altevogt. 1994. Eur. J. Immunol. 1994. 24:731.
4. Liu, Y., B. Jones, W. Brady, J. C. A. Janeway, and P. S. Linsley. 1992. Eur. J. Immunol. 22:2855.
5. Shields, J. G., S. H. Smith and R. E. Callard. 1987. In Leukocyte Typing III. A. J. McMichael, et. al., eds., Oxford University Press, Oxford. P. 488.
6. Kadmon, G., M. Eckert, M. Sammar, M. Schachner, and P. Altevogt. 1992. J. Cell Biol. 118:1245.
7. Aigner, S., M. Ruppert, M. Hubbe, M. Sammar, Z. Sthoeger, E. C. Butcher, D. Vestweber, and P. Altevogt. 1995. International Immunology 7:1557.
8. Ledbetter, J. A., and L. A. Herzenberg. 1979. Immunol. Rev. 47: 63.
9. Kariv, I., R. R. Hardy and K. Hayakawa. 1994. J. Exp. Med. 180:2419.
10. Hardy, R. R., C. E. Carmack, S. A. Shinton, J. D. Kemp, and K. Hayakawa. 1991. J. Exp. Med 173:1213.
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Product Notes
The CD24/HSA (Catalog #AAA673592) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The Rat Anti-Mouse CD24/HSA-FITC reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CD24/HSA can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry; Immunoprecipitation. Flow Cytometry Suggested Dilution: <= 2 ug/10^6 cells. Researchers should empirically determine the suitability of the CD24/HSA for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD24/HSA, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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