Rabbit anti-Human FBXO4 Polyclonal Antibody | anti-FBXO4 antibody

FBXO4 (FBX4, F-box Only Protein 4, DKFZp547N213, FLJ10141) (HRP)

Cat. Num. AAA6378266

• Gene Names: FBXO4; FBX4
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: FBXO4; Polyclonal Antibody; FBXO4 (FBX4; F-box Only Protein 4; DKFZp547N213; FLJ10141) (HRP); anti-FBXO4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human FBXO4.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-FBXO4 antibody: Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full length human FBXO4, aa1-387 (NP_036308.1).
• Immunogen Sequence: MAGSEPRSGTNSPPPPFSDWGRLEAAILSGWKTFWQSVSKERVARTTSREEVDEAASTLTRLPIDVQLYILSFLSPHDLCQLGSTNHYWNETVRDPILWRYFLLRDLPSWSSVDWKSLPDLEILKKPISEVTDGAFFDYMAVYRMCCPYTRRASKSSRPMYGAVTSFLHSLIIQNEPRFAMFGPGLEELNTSLVLSLMSSEELCPTAGLPQRQIDGIGSGVNFQLNNQHKFNILILYSTTRKERDRAREEHTSAVNKMFSRHNEGDDQQGSRYSVIPQIQKVCEVVDGFIYVANAEAHKRHEWQDEFSHIMAMTDPAFGSSGRPLLVLSCISQGDVKRMPCFYLAHELHLNLLNHPWLVQDTEAETLTGFLNGIEWILEEVESKRAR
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(FBXO4 rabbit polyclonal antibody. Western Blot analysis of FBXO4 expression in human pancreas.)

Western Blot (WB)

(Western Blot analysis of FBXO4 expression in transfected 293T cell line by FBXO4 polyclonal antibody. Lane 1: FBXO4 transfected lysate (44.1kD). Lane 2: Non-transfected lysate.)

Related Product Information for anti-FBXO4 antibody

Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex that mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Promotes ubiquitination of CCND1 and its subsequent proteasomal degradation. Recognizes TERF1 and promotes its ubiquitination together with UBE2D1.

Product Categories/Family for anti-FBXO4 antibody

Antibodies; Ubiquitin Ligases

NCBI and Uniprot Product Information

• NCBI GI #: 15834619
• NCBI GeneID: 26272
• NCBI Accession #: NP_036308.1
• NCBI GenBank Nucleotide #: NM_012176.2
• UniProt Accession #: Q9UKT5; Q68CU8; Q86VT8; Q9UK98
• Molecular Weight: 35,003 Da
• NCBI Official Full Name: F-box only protein 4 isoform 1
• NCBI Official Synonym Full Names: F-box protein 4
• NCBI Official Symbol: FBXO4
• NCBI Official Synonym Symbols: FBX4
• NCBI Protein Information: F-box only protein 4; F-box protein Fbx4
• UniProt Protein Name: F-box only protein 4
• Protein Family: F-box only protein
• UniProt Gene Name: FBXO4
• UniProt Synonym Gene Names: FBX4
• UniProt Entry Name: FBX4_HUMAN

NCBI Description

This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2014]

Uniprot Description

FBXO4: Substrate recognition component of a SCF (SKP1-CUL1-F- box protein) E3 ubiquitin-protein ligase complex that mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Promotes ubiquitination of CCND1 and its subsequent proteasomal degradation. Recognizes TERF1 and promotes its ubiquitination together with UBE2D1. Homodimer. Directly interacts with SKP1 and CUL1. Part of the SCF (SKP1-CUL1-F-box) E3 ubiquitin-protein ligase complex SCF(FBXO4) formed of CUL1, SKP1, RBX1 and FBXO4. Interacts with TERF1. Identified in a complex with CRYAB and CCND1. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin conjugating system; Ligase

Chromosomal Location of Human Ortholog: 5p12

Cellular Component: cytoplasm; SCF ubiquitin ligase complex; ubiquitin ligase complex

Molecular Function: protein binding; protein homodimerization activity; ubiquitin-protein ligase activity

Biological Process: ubiquitin-dependent protein catabolic process; SCF-dependent proteasomal ubiquitin-dependent protein catabolic process; protein polyubiquitination; positive regulation of protein ubiquitination; protein ubiquitination; telomere maintenance