Mouse CD23 Monoclonal Antibody | anti-CD23 antibody

MOUSE ANTI HUMAN CD23

Cat. Num. AAA214075

• Gene Names: FCER2; CD23; FCE2; CD23A; IGEBF; CLEC4J; BLAST-2
• Applications: Immunohistochemistry, Flow Cytometry, Functional Assay, Immunohistochemistry
• Synonyms: CD23; Monoclonal Antibody; MOUSE ANTI HUMAN CD23; anti-CD23 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 1B12
• Form/Format: Tissue Culture Supernatant - lyophilised
• Sequence Length: 320

• Applicable Applications for anti-CD23 antibody: Immunohistology Frozen, Flow cytometry (FC/FACS), Immunohistology Paraffin*
• Application Notes: Immunohistology - Frozen: Minimum Dilution: 1/20; Maximum Dilution: 1/40;; Immunohistology - Paraffin: Minimum Dilution: 1/20; Maximum Dilution: 1/40; Application Note: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
• Reconstitution: Reconstitute with 1.0ml distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. MyBioSource recommends that the vial is gently mixed after reconstitution. ; Perservative Stabilisers
• Immunogen: Recombinant human CD23; Histology Positive Control Tissue
• Target Species: Human
• Preparation and Storage: Prior to reconstitution store at 4 degree C. Following reconstitution store at -20 degree C if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 12 months from date of reconstitution.

Testing Data

(Staining of human tonsil. Formalin fixed, paraffin processed tissue. Mag. x 100 with Mouse anti human CD23 (MBS215238))

Related Product Information for anti-CD23 antibody

Mouse anti Human CD23 antibody, clone 1B12 recognizes the human CD23 cell surface antigen, a 45kDa glycoprotein that is a low affinity receptor for IgE. CD23 is expressed by peripheral B lymphocytes, cells of B cell chronic lymphocytic leukaemia and upon EBV transformed lymphoblastoid cells.

NCBI and Uniprot Product Information

• NCBI GI #: 334085205
• NCBI GeneID: 2208
• NCBI Accession #: NP_001193948.2
• NCBI GenBank Nucleotide #: NM_001207019.2
• UniProt Accession #: P06734
• Molecular Weight: 36,469 Da
• NCBI Official Full Name: low affinity immunoglobulin epsilon Fc receptor isoform b
• NCBI Official Synonym Full Names: Fc fragment of IgE, low affinity II, receptor for (CD23)
• NCBI Official Symbol: FCER2
• NCBI Official Synonym Symbols: CD23; FCE2; CD23A; IGEBF; CLEC4J; BLAST-2
• NCBI Protein Information: low affinity immunoglobulin epsilon Fc receptor; C-type lectin domain family 4, member J; CD23 antigen; fc-epsilon-RII; immunoglobulin E-binding factor; lymphocyte IgE receptor
• UniProt Protein Name: Low affinity immunoglobulin epsilon Fc receptor
• UniProt Gene Name: FCER2
• UniProt Synonym Gene Names: CD23A; CLEC4J; FCE2; IGEBF
• UniProt Entry Name: FCER2_HUMAN

NCBI Description

The protein encoded by this gene is a B-cell specific antigen, and a low-affinity receptor for IgE. It has essential roles in B cell growth and differentiation, and the regulation of IgE production. This protein also exists as a soluble secreted form, then functioning as a potent mitogenic growth factor. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.[provided by RefSeq, Jul 2011]

Uniprot Description

Low-affinity receptor for immunoglobulin E (IgE) and CR2/CD21. Has essential roles in the regulation of IgE production and in the differentiation of B-cells (it is a B-cell-specific antigen).