Mouse anti-Human CD22 Monoclonal Antibody | anti-CD22 antibody

Anti-Hu CD22 PE

• Gene Names: CD22; SIGLEC2; SIGLEC-2
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay
• Purity: The purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
• Synonyms: CD22; Monoclonal Antibody; Anti-Hu CD22 PE; SIGLEC2; SIGLEC-2; CD22 molecule; anti-CD22 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse IgG2b kappa
• Clone Number: S-HCL-1
• Specificity: The mouse monoclonal antibody S-HCL-1 (also known as S-HCL1) recognizes an extracellular epitope of CD22 (BL-CAM), a 130kDa type I transmembrane glycoprotein (immunoglobulin superfamily) expressed in the cytoplasm of pro-B and pre-B lymphocytes, and on the surface of mature and activated B lymphocytes; it is lost on plasma cells, peripheral blood T lymphocytes, granulocytes and monocytes.
• Purity/Purification: The purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
• Form/Format: Stabilizing phosphate buffered saline (PBS) solution containing 15mM sodium azide

• Applicable Applications for anti-CD22 antibody: Flow Cytometry (FC/FACS) (QC tested)
• Application Notes: FC/FACS: The reagent is designed for analysis of human blood cells using 10ul reagent/100ul of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests. Extracellular and intracellular staining.
• Conjugation: PE
• Immunogen: Whole hairy cell leukemia cells and membrane preparation
• Preparation and Storage: Store in the dark at 2-8 degree C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.

Flow Cytometry (FC/FACS)

(Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD22 (S-HCL-1) PE antibody (10 ul reagent / 100 ul of peripheral whole blood).)

Flow Cytometry (FC/FACS)

(Separation of human CD22 positive lymphocytes (red-filled) from human neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD22 (S-HCL-1) PE antibody (10 ul reagent / 100 ul of peripheral whole blood).)

Related Product Information for anti-CD22 antibody

CD22, also known as Siglec-2 (sialic acid-binding immunoglobulin-like lectin-2) is a transmembrane glycoprotein binding alpha2,6-linked sialic acid-bearing ligands. Intracellular domain of CD22 recruits protein tyrosine phosphatase SHP-1 through the immunoreceptor tyrosine-based inhibitory motifs (ITIMs), thus setting a treshold for B cell receptor-mediated activation. CD22 also regulates B-cell response by involvement in controlling the CD19/CD21-Src-family protein tyrosine kinase amplification pathway and CD40 signaling. CD22 exhibits hallmarks of clathrin-mediated endocytic pathway.

References

*Reineks EZ, Osei ES, Rosenberg A, Auletta J, Meyerson HJ: CD22 expression on blastic plasmacytoid dendritic cell neoplasms and reactivity of anti-CD22 antibodies to peripheral blood dendritic cells. Cytometry B Clin Cytom. 2009 Jul;76(4):237-48, URL: https://www.ncbi.nlm.nih.gov/pubmed/term=Reineks+,*James SE, Greenberg PD, Jensen MC, Lin Y, Wang J, Till BG, Raubitschek AA, Forman SJ, Press OW: Antigen sensitivity of CD22-specific chimeric TCR is modulated by target epitope distance from the cell membrane. J Immunol. 2008 May 15;180(10):7028-38., URL: http://www.ncbi.nlm.nih.gov/pubmed/18453625,*Huang J, Fan G, Zhong Y, Gatter K, Braziel R, Gross G, Bakke A: Diagnostic usefulness of aberrant CD22 expression in differentiating neoplastic cells of B-Cell chronic lymphoproliferative disorders from admixed benign B cells in four-color multiparameter flow cytometry. Am J Clin Pathol. 2005 Jun;123(6):826-32., URL: http://www.ncbi.nlm.nih.gov/pubmed/15899772,*Foon KA, Todd RF 3rd: Immunologic classification of leukemia and lymphoma. Blood. 1986 Jul;68(1):1-31., URL: http://www.ncbi.nlm.nih.gov/pubmed/2941082
*Walker JA, Smith KG: CD22: an inhibitory enigma. Immunology. 2007 Dec 7, URL: https://www.ncbi.nlm.nih.gov/pubmed/18067554ordin,*Tateno H, Li H, Schur MJ, Bovin N, Crocker PR, Wakarchuk WW, Paulson JC: Distinct endocytic mechanisms of CD22 (Siglec-2) and Siglec-F reflect roles in cell signaling and innate immunity. Mol Cell Biol. 2007 Aug;27(16):5699-710., URL: https://www.ncbi.nlm.nih.gov/pubmed/17562860ordin,*Tedder TF, Poe JC, Haas KM: CD22: A MultiFunctional Application (FUNC)tional Receptor That Regulates B Lymphocyte Survival and Signal Transduction. Adv Immunol. 2005;88:1-50., URL: https://www.ncbi.nlm.nih.gov/pubmed/16227086ordin

NCBI and Uniprot Product Information

• NCBI GI #: 297374825
• NCBI GeneID: 933
• NCBI Accession #: NP_001172028.1
• NCBI GenBank Nucleotide #: NM_001185099.1
• UniProt Accession #: P20273; O95699; O95701; O95702; O95703; Q01665; Q92872; Q92873; Q9UQA6; Q9UQA7; Q9UQA8; F5H7U3
• Molecular Weight: 95,348 Da
• NCBI Official Full Name: B-cell receptor CD22 isoform 2
• NCBI Official Synonym Full Names: CD22 molecule
• NCBI Official Symbol: CD22
• NCBI Official Synonym Symbols: SIGLEC2; SIGLEC-2
• NCBI Protein Information: B-cell receptor CD22; BL-CAM; CD22 antigen; T-cell surface antigen Leu-14; B-lymphocyte cell adhesion molecule; sialic acid binding Ig-like lectin 2; sialic acid-binding Ig-like lectin 2
• UniProt Protein Name: B-cell receptor CD22
• Protein Family: CD226 antigen
• UniProt Gene Name: CD22
• UniProt Synonym Gene Names: SIGLEC2; BL-CAM; Siglec-2
• UniProt Entry Name: CD22_HUMAN

Uniprot Description

CD22: Mediates B-cell B-cell interactions. May be involved in the localization of B-cells in lymphoid tissues. Binds sialylated glycoproteins; one of which is CD45. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site can be masked by cis interactions with sialic acids on the same cell surface. Upon ligand induced tyrosine phosphorylation in the immune response seems to be involved in regulation of B-cell antigen receptor signaling. Plays a role in positive regulation through interaction with Src family tyrosine kinases and may also act as an inhibitory receptor by recruiting cytoplasmic phosphatases via their SH2 domains that block signal transduction through dephosphorylation of signaling molecules. Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell surface; Membrane protein, integral

Chromosomal Location of Human Ortholog: 19q13.1

Cellular Component: integral to plasma membrane; external side of plasma membrane

Molecular Function: protein binding; carbohydrate binding

Biological Process: cell adhesion