Flow Cytometry (FC/FACS)
(Flow cytometric analysis of Human CD22 expression on human whole blood lymphocytes. Cells were stained with FITC-conjugated anti-Human CD22 and APC-conjugated anti-Human CD19. The dot plots were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes.)
Mouse anti-Human CD22 Monoclonal Antibody | anti-CD22 antibody
Anti-CD22 Antibody (FITC), Mouse Monoclonal
Gene Names
CD22; SIGLEC2; SIGLEC-2
Reactivity
Human
Applications
Flow Cytometry, Functional Assay
Purity
Protein A
Synonyms
CD22; Monoclonal Antibody; Anti-CD22 Antibody (FITC); Mouse Monoclonal; CD22/Siglec-2 Antibody (FITC); Mouse MAb; CD22 molecule; Anti-SIGLEC-2 Antibody; Anti-SIGLEC2 Antibody; anti-CD22 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
Mouse IgG1
Clone Number
13
Specificity
Human CD22/Siglec-2
Purity/Purification
Protein A
Form/Format
Liquid; Aqueous solution containing 0.5% BSA and 0.09% sodium azide
Concentration
5ul/Test, 0.1mg/ml (varies by lot)
Applicable Applications for anti-CD22 antibody
Flow Cytometry (FC/FACS/FCM)
Immunogen
Recombinant Human CD22/Siglec-2 Protein
Conjugation
FITC
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD22/Siglec-2 (rh CD22/Siglec-2) and conjugated with FITC under optimum conditions, the unreacted FITC was removed.
Preparation and Storage
This antibody can be stored at 2-8 degree C for twelve months without detectable loss of activity. Protected from prolonged exposure to light. Do not freeze! Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal.
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of Human CD22 expression on human whole blood lymphocytes. Cells were stained with FITC-conjugated anti-Human CD22 and APC-conjugated anti-Human CD19. The dot plots were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of Human CD22 expression on human whole blood lymphocytes. Cells were stained with FITC-conjugated anti-Human CD22 and APC-conjugated anti-Human CD19. The dot plots were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes.)
Related Product Information for anti-CD22 antibody
CD22 is a member of the immunoglobulin superfamily, SIGLEC family of lectins. It is first expressed in the cytoplasm of pro-B and pre-B cells, and on the surface as B cells mature to become IgD+. CD22 serves as an adhesion receptor for sialic acid-bearing ligands expressed on erythrocytes and all leukocyte classes. In addition to its potential role as a mediator of intercellular interactions, signal transduction through CD22 can activate B cells and modulate antigen receptor signaling in vitro. The phenotype of CD22-deficient mice suggests that CD22 is primarily involved in the generation of mature B cells within the bone marrow, blood, and marginal zones of lymphoid tissues. CD22 recruits the tyrosine phosphatase Src homology 2 domain-containing phosphatase 1 (SHP-1) to immunoreceptor tyrosine-based inhibitory motifs (ITIMs) and inhibits B-cell receptor (BCR)-induced Ca2+ signaling on normal B cells. CD22 interacts specifically with ligands carrying alpha2-6-linked sialic acids. As an inhibitory coreceptor of the B-cell receptor (BCR), CD22 plays a critical role in establishing signalling thresholds for B-cell activation. Like other coreceptors, the ability of CD22 to modulate B-cell signalling is critically dependent upon its proximity to the BCR, and this in turn is governed by the binding of its extracellular domain to alpha2, 6-linked sialic acid ligands. However, genetic studies in mice reveal that some CD22 functions are regulated by ligand binding, whereas other functions are ligand-independent and may only require expression of an intact CD22 cytoplasmic domain at the B-cell surface. CD19 regulates CD22 phosphorylation by augmenting Lyn kinase activity, while CD22 inhibits CD19 phosphorylation via SHP-1.
Product Categories/Family for anti-CD22 antibody
References
Tedder TF, et al. (1997) CD22, a B lymphocyte-specific adhesion molecule that regulates antigen receptor signaling. Annu Rev Immunol. 15: 481-504.
Tedder TF, et al. (2005) CD22: a multifunctional receptor that regulates B lymphocyte survival and signal transduction. Adv Immunol. 88: 1-50.
Fujimoto M, et al. (2007) B cell signaling and autoimmune diseases: CD19/CD22 loop as a B cell signaling device to regulate the balance of autoimmunity. J Dermatol Sci. 46(1): 1-9.
Walker JA, et al. (2008) CD22: an inhibitory enigma. Immunology. 123(3): 314-25.
Nitschke L. (2009) CD22 and Siglec-G: B-cell inhibitory receptors with distinct functions. Immunol Rev. 230(1): 128-43.
Tedder TF, et al. (2005) CD22: a multifunctional receptor that regulates B lymphocyte survival and signal transduction. Adv Immunol. 88: 1-50.
Fujimoto M, et al. (2007) B cell signaling and autoimmune diseases: CD19/CD22 loop as a B cell signaling device to regulate the balance of autoimmunity. J Dermatol Sci. 46(1): 1-9.
Walker JA, et al. (2008) CD22: an inhibitory enigma. Immunology. 123(3): 314-25.
Nitschke L. (2009) CD22 and Siglec-G: B-cell inhibitory receptors with distinct functions. Immunol Rev. 230(1): 128-43.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
95,348 Da
NCBI Official Full Name
B-cell receptor CD22 isoform 2
NCBI Official Synonym Full Names
CD22 molecule
NCBI Official Symbol
CD22
NCBI Official Synonym Symbols
SIGLEC2; SIGLEC-2
NCBI Protein Information
B-cell receptor CD22; BL-CAM; CD22 antigen; T-cell surface antigen Leu-14; B-lymphocyte cell adhesion molecule; sialic acid binding Ig-like lectin 2; sialic acid-binding Ig-like lectin 2
UniProt Protein Name
B-cell receptor CD22
Protein Family
UniProt Gene Name
CD22
UniProt Synonym Gene Names
SIGLEC2; BL-CAM; Siglec-2
UniProt Entry Name
CD22_HUMAN
Uniprot Description
CD22: Mediates B-cell B-cell interactions. May be involved in the localization of B-cells in lymphoid tissues. Binds sialylated glycoproteins; one of which is CD45. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site can be masked by cis interactions with sialic acids on the same cell surface. Upon ligand induced tyrosine phosphorylation in the immune response seems to be involved in regulation of B-cell antigen receptor signaling. Plays a role in positive regulation through interaction with Src family tyrosine kinases and may also act as an inhibitory receptor by recruiting cytoplasmic phosphatases via their SH2 domains that block signal transduction through dephosphorylation of signaling molecules. Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Cell surface; Membrane protein, integral
Chromosomal Location of Human Ortholog: 19q13.1
Cellular Component: integral to plasma membrane; external side of plasma membrane
Molecular Function: protein binding; carbohydrate binding
Biological Process: cell adhesion
Research Articles on CD22
Similar Products
Product Notes
The CD22 cd22 (Catalog #AAA8106025) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-CD22 Antibody (FITC), Mouse Monoclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD22 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS/FCM). Researchers should empirically determine the suitability of the CD22 cd22 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD22, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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