Mouse anti-Human CD2 Monoclonal Antibody | anti-CD2 antibody
Mouse anti Human CD2
Reactivity
Human
Applications
Flow Cytometry, Functional Assay
Purity
Protein A/G Chromatography
Synonyms
CD2; Monoclonal Antibody; Mouse anti Human CD2; T-cell surface antigen CD2; CD2 cytoplasmic domain-binding protein; anti-CD2 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
T6.3
Purity/Purification
Protein A/G Chromatography
Form/Format
Provided as solution in phosphate buffered saline with 0.08% sodium azide
Applicable Applications for anti-CD2 antibody
Flow Cytometry (FC/FACS)
Application Notes
PBMC: Add 10 µl of antibody to 1 million PBMCs in 100 µl PBS. Mix gently and incubate for 15 minutes at 2 to 8oC. Wash twice with PBS, incubate with an appropriate concentratio with a fluorochrome conjugated secondary antibody, wash again and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze.
WHOLE BLOOD: Add10 µl of antibody to 100 µl of whole blood. Mix gently and incubate for 15 minutes at room temperature 20oC. Lyse the whole blood. Wash once with PBS, incubate with an appropriate concentratio with a fluorochrome conjugated secondary antibody, wash again and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze Functional Analysis: Flow cytometry staining
WHOLE BLOOD: Add10 µl of antibody to 100 µl of whole blood. Mix gently and incubate for 15 minutes at room temperature 20oC. Lyse the whole blood. Wash once with PBS, incubate with an appropriate concentratio with a fluorochrome conjugated secondary antibody, wash again and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze Functional Analysis: Flow cytometry staining
Source Note
Derived from hybridization of mouse Sp2/0 myeloma cells with spleen cells from BALB/c mice immunized with T Lymphocytes activated by a mixed lymphocyte culture.
Preparation and Storage
Product should be stored at -20 degree C. Aliquot to avoid freeze/thaw cycles. Product Stability: Antibodies are stable for one year from purchase if stored frozen
Related Product Information for anti-CD2 antibody
Mouse anti human CD2 can be used for identification of human T cells and a subset of NK cells associated with the receptor for sheep erythocytes rosettes expressing the 45-50,000 M.W. surface antigen.
References
1. An Improved Rosetting Assay for Detection of Human T Lymphocytes. Kaplan M.E., Clark C., J. Immunol. Methods 1974, 5,131.
2. Structural and functional characterization of the CD2 immunoadhesion domain. Evidence for inclusion of CD2 in an alpha-beta protein folding class.Recny M.A., Neidhardt E.A., Sayre P.H., Ciardelli T.L., Reinherz E.L., J. Biol. Chem. 1990 May 2;265(15):8541-9.
3. Partial deletions of the cytoplasm domain of CD2 result in a partial defect in signal transduction. Bierer B.E., Bogart R.E., Burakoff S.J., J. Immunol. 1990 Feb. :144(3):785.
4. Functional CD2 mutants unable to bind to, or be stimulated by, LFA-3. Wolff H.L., Burakoff S.J., Bierer B.E., J. Immunol. 1990 Feb. 1;144(4):1215-20.
5. Association of CD2 and CD45 on human T lymphocytes. Schraven B., Samstag Y., Altevogt P., Meuer S.C., Nature 1990 May ;345(6270):71-4 .
6. Gardner, J.P. et al. 'Robust, but transient expression of adeno-associated virus-transduced genes during human t lymphopoisis.' Blood, 1997, 90, 4854-4864.
2. Structural and functional characterization of the CD2 immunoadhesion domain. Evidence for inclusion of CD2 in an alpha-beta protein folding class.Recny M.A., Neidhardt E.A., Sayre P.H., Ciardelli T.L., Reinherz E.L., J. Biol. Chem. 1990 May 2;265(15):8541-9.
3. Partial deletions of the cytoplasm domain of CD2 result in a partial defect in signal transduction. Bierer B.E., Bogart R.E., Burakoff S.J., J. Immunol. 1990 Feb. :144(3):785.
4. Functional CD2 mutants unable to bind to, or be stimulated by, LFA-3. Wolff H.L., Burakoff S.J., Bierer B.E., J. Immunol. 1990 Feb. 1;144(4):1215-20.
5. Association of CD2 and CD45 on human T lymphocytes. Schraven B., Samstag Y., Altevogt P., Meuer S.C., Nature 1990 May ;345(6270):71-4 .
6. Gardner, J.P. et al. 'Robust, but transient expression of adeno-associated virus-transduced genes during human t lymphopoisis.' Blood, 1997, 90, 4854-4864.
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Product Notes
The CD2 (Catalog #AAA574004) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse anti Human CD2 reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD2 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS). PBMC: Add 10 µl of antibody to 1 million PBMCs in 100 µl PBS. Mix gently and incubate for 15 minutes at 2 to 8oC. Wash twice with PBS, incubate with an appropriate concentratio with a fluorochrome conjugated secondary antibody, wash again and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add10 µl of antibody to 100 µl of whole blood. Mix gently and incubate for 15 minutes at room temperature 20oC. Lyse the whole blood. Wash once with PBS, incubate with an appropriate concentratio with a fluorochrome conjugated secondary antibody, wash again and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze Functional Analysis: Flow cytometry staining. Researchers should empirically determine the suitability of the CD2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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