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Flow Cytometry (FC/FACS) (Peripheral blood lymphocytes stained with CD56 PE Conjugated Antibody .)

Mouse anti-Human CD56 Antibody | anti-CD56 antibody

CD56 PE

Gene Names
NCAM1; CD56; NCAM; MSK39
Reactivity
Human
Applications
Flow Cytometry, Functional Assay
Synonyms
CD56; CD56 PE; CD56PE; Mouse Single-Color Reagent C5.9; NCAM; Neural Cell Adhesion Molecule; anti-CD56 antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human
Isotype
IgG2b
Clone Number
C5.9
Form/Format
Form: RPE
Formulation: Provided as sterile filtered solution in phosphate buffered saline with 0.08% sodium azide and 0.2% carrier protein
Concentration
Titered for flow cytometry (varies by lot)
Sequence Length
848
Applicable Applications for anti-CD56 antibody
Flow Cytometry (FC/FACS)
Volume
1 mL
Use
PBMC: Add 10ul of MAB/106 PBMC in 100ul PBS. Mix gently and incubate for 15 minutes at 2 degree C to 8 degree C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add 10ul of MAB/100ul of whole blood. Mix gently and incubate for 15 minutes at room temperature 20°C. Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturer's instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.
Stability
Reagents are stable for the period shown on the vial label when stored properly.
Preparation and Storage
Product should be stored at 4-8°C. DO NOT FREEZE
Shipping:Room temperature

Flow Cytometry (FC/FACS)

(Peripheral blood lymphocytes stained with CD56 PE Conjugated Antibody .)

Flow Cytometry (FC/FACS) (Peripheral blood lymphocytes stained with CD56 PE Conjugated Antibody .)
Related Product Information for anti-CD56 antibody
CD56, M.W. 150,000, is expressed on approximately 10-25% of human peripheral blood lymphocytes. CD56 (NKH-1) is expressed on human peripheral blood natural killer cells, representing a pan NK-cell antigen. Expressed on non-MHC-restricted cytotoxic T cells.
Product Categories/Family for anti-CD56 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
neural cell adhesion molecule 1 isoform 1
NCBI Official Synonym Full Names
neural cell adhesion molecule 1
NCBI Official Symbol
NCAM1
NCBI Official Synonym Symbols
CD56; NCAM; MSK39
NCBI Protein Information
neural cell adhesion molecule 1; neural cell adhesion molecule, NCAM; antigen recognized by monoclonal antibody 5.1H11
UniProt Protein Name
Neural cell adhesion molecule 1
UniProt Gene Name
NCAM1
UniProt Synonym Gene Names
NCAM; N-CAM-1; NCAM-1
UniProt Entry Name
NCAM1_HUMAN

NCBI Description

This gene encodes a cell adhesion protein which is a member of the immunoglobulin superfamily. The encoded protein is involved in cell-to-cell interactions as well as cell-matrix interactions during development and differentiation. The encoded protein has been shown to be involved in development of the nervous system, and for cells involved in the expansion of T cells and dendritic cells which play an important role in immune surveillance. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2011]

Uniprot Description

Function: This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc.

Subcellular location: Isoform 1: Cell membrane; Single-pass type I membrane protein. Isoform 2: Cell membrane; Single-pass type I membrane protein. Isoform 3: Cell membrane; Lipid-anchor › GPI-anchor. Isoform 4: Cell membrane; Lipid-anchor › GPI-anchor

Potential. Isoform 5: Secreted. Isoform 6: Secreted

Potential.

Sequence similarities: Contains 2 fibronectin type-III domains.Contains 5 Ig-like C2-type (immunoglobulin-like) domains.

Sequence caution: The sequence BAD92680.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Research Articles on CD56

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Product Notes

The CD56 ncam1 (Catalog #AAA396540) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The CD56 PE reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD56 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS). Researchers should empirically determine the suitability of the CD56 ncam1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD56, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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