Mouse anti-Human. CD2 Monoclonal Antibody | anti-CD2 antibody

CD2

Cat. Num. AAA438109

• Gene Names: CD2; T11; SRBC; LFA-2
• Reactivity: Human.
• Applications: ELISA, Flow Cytometry, Functional Assay, Immunofluorescence, Immunoprecipitation, Immunohistochemistry
• Synonyms: CD2; Monoclonal Antibody; Erythrocyte receptor; LFA-2; LFA-3 receptor; Ly-37; Lymphocyte function antigen 2; Rosette receptor; SRBC; T-cell surface antigen CD2; T-cell surface antigen T11/Leu-5; T11; anti-CD2 antibody

• Host: Mouse
• Reactivity: Human.
• Clonality: Monoclonal
• Isotype: IgG2b, kappa
• Clone Number: BH1
• Specificity: CD2 interacts through its amino-terminal domain with the extracellular domain of CD58 (also designated CD2 ligand) to mediate cell adhesion. CD2/CD58 binding can enhance antigen-specific T cell activation. CD2 is a transmembrane glycoprotein that is expressed on peripheral blood T lymphocytes, NK cells and thymocytes. CD58 is a heavily glycosylated protein with a broad tissue distribution in hematopoietic and other cells, including endothelium. Interaction between CD2 and its counter receptor LFA3 (CD58) on opposing cells optimizes immune system recognition, thereby facilitating communication between helper T lymphocytes and antigen-presenting cells, as well as between cytolytic effectors and target cells.
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 351

• Applicable Applications for anti-CD2 antibody: ELISA (EIA), Functional Studies, Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistology (IH)
• Application Notes: ELISA (For coating, order Ab without BSA); Functional Studies (Order Ab without Azide); Flow Cytometry (0.5-1ug/million cells); Immunofluorescence (0.5-1ug/ml); Immunoprecipitation (0.5-1ug/500ug protein lysate); Immunohistology (Frozen) (0.5-1ug/ml for 30 minutes at RT) Optimal dilution for a specific application should be determined.
• Localization: Cell surface
• Positive Control: 293T or Jurkat cells. Lymph node and tonsil
• Human Chromosomal Location: 1p13.1
• Immunogen: Human CD2 protein
• Quantity and Buffer: 0.5 ml at 200ug/ml with BSA and azide
• Preparation and Storage: Store at 2 to 8 degree C. Antibody is stable for 24 months.

References

Wang MY et. al. An effective immuno-magnetic method for bone marrow purging in T cell malignancies. Bone Marrow Transplant. 1992;9(5):319-23.

NCBI and Uniprot Product Information

• NCBI GI #: 156071472
• NCBI GeneID: 914
• NCBI Accession #: NP_001758.2
• NCBI GenBank Nucleotide #: NM_001767.3
• UniProt Accession #: P06729; Q96TE5
• Molecular Weight: 50kDa
• NCBI Official Full Name: T-cell surface antigen CD2
• NCBI Official Synonym Full Names: CD2 molecule
• NCBI Official Symbol: CD2
• NCBI Official Synonym Symbols: T11; SRBC; LFA-2
• NCBI Protein Information: T-cell surface antigen CD2; LFA-3 receptor; rosette receptor; erythrocyte receptor; lymphocyte-function antigen-2; T-cell surface antigen T11/Leu-5; CD2 antigen (p50), sheep red blood cell receptor
• UniProt Protein Name: T-cell surface antigen CD2
• Protein Family: CD2
• UniProt Gene Name: CD2
• UniProt Synonym Gene Names: SRBC
• UniProt Entry Name: CD2_HUMAN

NCBI Description

CD2 is a surface antigen of the human T-lymphocyte lineage that is expressed on all peripheral blood T cells (summarized by Sewell et al., 1986 [PubMed 3490670]). It is one of the earliest T-cell markers, being present on more than 95% of thymocytes; it is also found on some natural killer cells but not on B lymphocytes. Monoclonal antibodies directed against CD2 inhibit the formation of rosettes with sheep erythrocytes, indicating that CD2 is the erythrocyte receptor or is closely associated with it.[supplied by OMIM, Jul 2010]

Uniprot Description

CD2: CD2 interacts with lymphocyte function-associated antigen (LFA-3) and CD48/BCM1 to mediate adhesion between T-cells and other cell types. CD2 is implicated in the triggering of T- cells, the cytoplasmic domain is implicated in the signaling function.

Protein type: Membrane protein, integral; Apoptosis; Cell surface

Chromosomal Location of Human Ortholog: 1p13.1

Cellular Component: internal side of plasma membrane; cell surface; anchored to plasma membrane; integral to plasma membrane; extracellular region; plasma membrane; intercellular junction; external side of plasma membrane

Molecular Function: protein binding; protein homodimerization activity; receptor activity; receptor binding

Biological Process: heterotypic cell-cell adhesion; cell-cell adhesion; cell surface receptor linked signal transduction; T cell activation; apoptosis; natural killer cell activation; regulation of T cell differentiation; lipid raft polarization; blood coagulation; positive regulation of tumor necrosis factor production; leukocyte migration; positive regulation of myeloid dendritic cell activation