Mouse anti-Human, Mouse CD1a / HTA1 Monoclonal Antibody | anti-CD1A antibody

CD1a / HTA1 (Mature Langerhans Cells Marker) Mouse Monoclonal Antibody

Cat. Num. AAA438742

• Gene Names: CD1A; R4; T6; CD1; FCB6; HTA1
• Reactivity: Human, Mouse
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence
• Synonyms: CD1a / HTA1; Monoclonal Antibody; CD1a / HTA1 (Mature Langerhans Cells Marker) Mouse Monoclonal Antibody; Cortical thymocyte antigen CD1A; Epidermal dendritic cell marker CD1a; FCB6; HTA1; T cell surface antigen T6 / Leu 6; T-Cell Surface Glycoprotein CD1A; anti-CD1A antibody

• Host: Mouse
• Reactivity: Human, Mouse
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone Number: CBT6
• Specificity: At least five CD1 genes (CD1a, b, c, d, and e) are identified. CD1 proteins have been demonstrated to restrict T cell response to non-peptide lipid and glycolipid antigens and play a role in non-classical antigen presentation. CD1a is a non-polymorphic MHC Class 1 related cell surface glycoprotein, expressed in association with Beta-2 microglobulin. Anti-CD1a labels Langerhans cell histiocytosis (Histiocytosis X), extranodal histiocytic sarcoma, a subset of T-lymphoblastic lymphoma/leukemia, and interdigitating dendritic cell sarcoma of the lymph node. When combined with antibodies against TTF-1 and CD5, anti-CD1a is useful in distinguishing between pulmonary and thymic neoplasms since CD1a is consistently expressed in thymic lymphocytes in both typical and atypical thymomas, but only focally in 1/6 of thymic carcinomas and not in lymphocytes in pulmonary neoplasms. Anti-CD1a is reported to be a new marker for perivascular epithelial cell tumor (PEComa).
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 316

• Applicable Applications for anti-CD1A antibody: Flow Cytometry (FC/FACS), Immunofluorescence (IF)
• Application Notes: Flow Cytometry (0.5-1ug/million cells in 0.1ml); Immunofluorescence (1-2ug/ml)
• Cellular Localization: Cell Surface
• Immunogen: Human CD1a
• Hu-Chromosome Location: 1q23.1
• Positive Control: MOLT-4 cells. Paracortex in a tonsil or a reactive lymph node.
• Preparation and Storage: Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

References

van de Rijn M et al. J Immunol 1983, 131(2):851-5 Lerch PG et al. Hum Immunol 1983, 6(1):13-30 Knapp W. et al. (eds) Leukocyte Typing IV, p251-263, Oxford University Press, Oxford, 1989 Khalili-Shirazi A, et al. J Neurol Sci 1998,158(2):154-163. Maher JK and Kronenberg M.. Curr Opin Immunol 1997, 9(4):456-461 Blumberg RS et al. Immunol Rev 1995, 147:5-29. Salamone MC et al. Dis Markers 1990, 8(5):275-281

NCBI and Uniprot Product Information

• NCBI GI #: 1002167153
• NCBI GeneID: 909
• NCBI Accession #: NP_001307581.1
• NCBI GenBank Nucleotide #: NM_001320652.1
• UniProt Accession #: P06126; Q13962; Q5TDJ8; Q9UMM4; Q9Y5M5; D3DVD7
• Molecular Weight: 49kDa
• NCBI Official Full Name: T-cell surface glycoprotein CD1a isoform 2
• NCBI Official Synonym Full Names: CD1a molecule
• NCBI Official Symbol: CD1A
• NCBI Official Synonym Symbols: R4; T6; CD1; FCB6; HTA1
• NCBI Protein Information: T-cell surface glycoprotein CD1a
• UniProt Protein Name: T-cell surface glycoprotein CD1a
• Protein Family: T-cell surface glycoprotein
• UniProt Gene Name: CD1A
• UniProt Synonym Gene Names: hTa1 thymocyte antigen
• UniProt Entry Name: CD1A_HUMAN

NCBI Description

This gene encodes a member of the CD1 family of transmembrane glycoproteins, which are structurally related to the major histocompatibility complex (MHC) proteins and form heterodimers with beta-2-microglobulin. The CD1 proteins mediate the presentation of primarily lipid and glycolipid antigens of self or microbial origin to T cells. The human genome contains five CD1 family genes organized in a cluster on chromosome 1. The CD1 family members are thought to differ in their cellular localization and specificity for particular lipid ligands. The protein encoded by this gene localizes to the plasma membrane and to recycling vesicles of the early endocytic system. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2016]

Uniprot Description

CD1A: Antigen-presenting protein that binds self and non-self lipid and glycolipid antigens and presents them to T-cell receptors on natural killer T-cells.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 1q23.1

Cellular Component: plasma membrane

Molecular Function: beta-2-microglobulin binding; endogenous lipid antigen binding; exogenous lipid antigen binding; protein binding

Biological Process: antigen processing and presentation, exogenous lipid antigen via MHC class Ib; regulation of immune response