Rabbit anti-Human CD1a/HTA1 Polyclonal Antibody | anti-CD1A antibody

CD1a/HTA1 (Mature Langerhans Cells Marker)

Cat. Num. AAA4381376

• Gene Names: CD1A; R4; T6; CD1; FCB6; HTA1
• Reactivity: Human
• Applications: Immunohistochemistry
• Purity: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Synonyms: CD1a/HTA1; Polyclonal Antibody; CD1a/HTA1 (Mature Langerhans Cells Marker); Cortical thymocyte antigen CD1A; Epidermal dendritic cell marker CD1a antibody; FCB6; HTA1; T cell surface antigen T6/Leu 6; T-Cell Surface Glycoprotein CD1A; anti-CD1A antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: At least five CD1 genes (CD1a, b, c, d, and e) are identified. CD1 proteins have been demonstrated to restrict T cell response to non-peptide lipid and glycolipid antigens and play a role in non-classical antigen presentation. CD1a is a non-polymorphic MHC Class 1 related cell surface glycoprotein, expressed in association with Beta-2 microglobulin. Anti-CD1a labels Langerhans cell histiocytosis (Histiocytosis X), extranodal histiocytic sarcoma, a subset of T-lymphoblastic lymphoma/leukemia, and interdigitating dendritic cell sarcoma of the lymph node. When combined with antibodies against TTF-1 and CD5, anti-CD1a is useful in distinguishing between pulmonary and thymic neoplasms since CD1a is consistently expressed in thymic lymphocytes in both typical and atypical thymomas, but only focally in 1/6 of thymic carcinomas and not in lymphocytes in pulmonary neoplasms. Anti-CD1a is reported to be a new marker for perivascular epithelial cell tumor (PEComa).
• Purity/Purification: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Form/Format: 200ug/ml of Ab Purified by Protein A Column. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 327

• Applicable Applications for anti-CD1A antibody: Immunohistochemistry (IHC) Paraffin
• Application Notes: Immunohistochemistry (Formalin-fixed) (0.5-1ug/ml for 30 min at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
• Positive Control: MOLT-4 cells. Paracortex in a tonsil or a reactive lymph node.
• Immunogen: Recombinant human CD1a protein
• Cellular Localization: Cell Surface
• Hu-chromosome Location: 1q23.1
• Preparation and Storage: Antibody with azide: store at 2 to 8 degree C.; Antibody without azide: store at -20 to -80 degree C.; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded human Skin stained with CD1a Rabbit Polyclonal Antibody.)

References

1. Cassaday, RD. et al. 2007. Clin. Cancer Res. 13: 540-549. 2. Matsuda, A. et al. 2009. Invest. Ophthalmol. Vis. Sci. 50: 2871-2877. 3. Gulubova, M. et al. 2008. Clin. Exp. Metastasis. 25: 777-785. 4. McNally, AK. et al. 2011. Exp. Mol. Pathol. 91: 673-681.

NCBI and Uniprot Product Information

• NCBI GI #: 288558852
• NCBI GeneID: 909
• UniProt Accession #: P06126
• Molecular Weight: 49kDa
• NCBI Official Full Name: T-cell surface glycoprotein CD1a
• NCBI Official Synonym Full Names: CD1a molecule
• NCBI Official Symbol: CD1A
• NCBI Official Synonym Symbols: R4; T6; CD1; FCB6; HTA1
• NCBI Protein Information: T-cell surface glycoprotein CD1a
• UniProt Protein Name: T-cell surface glycoprotein CD1a
• Protein Family: T-cell surface glycoprotein
• UniProt Gene Name: CD1A
• UniProt Synonym Gene Names: hTa1 thymocyte antigen

NCBI Description

This gene encodes a member of the CD1 family of transmembrane glycoproteins, which are structurally related to the major histocompatibility complex (MHC) proteins and form heterodimers with beta-2-microglobulin. The CD1 proteins mediate the presentation of primarily lipid and glycolipid antigens of self or microbial origin to T cells. The human genome contains five CD1 family genes organized in a cluster on chromosome 1. The CD1 family members are thought to differ in their cellular localization and specificity for particular lipid ligands. The protein encoded by this gene localizes to the plasma membrane and to recycling vesicles of the early endocytic system. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2016]

Uniprot Description

Antigen-presenting protein that binds self and non-self lipid and glycolipid antigens and presents them to T-cell receptors on natural killer T-cells.