Mouse anti-Human CD1a Monoclonal Antibody | anti-CD1a antibody

Anti-Hu CD1a FITC

• Gene Names: CD1A; R4; T6; CD1; FCB6; HTA1
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay
• Purity: The purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
• Synonyms: CD1a; Monoclonal Antibody; Anti-Hu CD1a FITC; T6; Leu-6; HTA1; FCB6; CD1a molecule; anti-CD1a antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse IgG2b kappa
• Clone Number: SK9
• Specificity: The mouse monoclonal antibody SK9 recognizes an extracellular epitope of CD1a (T6), a 49kDa polypeptide associated with beta2-microglobulin expressed on cortical thymocytes (strongly), Langerhans cells, dendritic cells and some T cell leukemias and lymphomas.
• Purity/Purification: The purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
• Form/Format: Stabilizing phosphate buffered saline (PBS) solution containing 15mM sodium azide

• Applicable Applications for anti-CD1a antibody: Flow Cytometry (FC/FACS) (QC tested)
• Application Notes: FC/FACS: The reagent is designed for analysis of human blood cells using 4ul reagent/100ul of whole blood or 10⁶ cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
• Conjugation: FITC
• Immunogen: Human thymocytes
• Preparation and Storage: Store in the dark at 2-8 degree C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.

Related Product Information for anti-CD1a antibody

CD1a, together with CD1b and c, belongs to group 1 of CD1 glycoproteins. These proteins serve as antigen-presenting molecules for a subset of T cells that responds to specific lipids and glycolipids found in the cell walls of bacterial pathogens or self-glycolipid antigens such as gangliosides, and they have also roles in antiviral immunity. Unlike CD1b, CD1a is excluded from late endosomal compartments and instead traffics independently in the recycling pathway of the early endocytic system, and CD1a antigen presentation is independent upon vesicular acidification.

References

*Kader M, Smith AP, Guiducci C, Wonderlich ER, Normolle D, Watkins SC, Barrat FJ, Barratt-Boyes SM: Blocking TLR7- and TLR9-mediated IFN-alpha production by plasmacytoid dendritic cells does not diminish immune activation in early SIV infection. PLoS Pathog. 2013;9(7):e1003530., URL: http://www.ncbi.nlm.nih.gov/pubmed/term=kader+m+s,*Awasthi S, Wolf R, White G: Ontogeny and phagocytic Functional Application (FUNC)tion of baboon lung dendritic cells. Immunol Cell Biol. 2009 Jul;87(5):419-27., URL: http://www.ncbi.nlm.nih.gov/pubmed/19223853,*Tenca C, Merlo A, Zarcone D, Saverino D, Bruno S, De Santanna A, Ramarli D, Fabbi M, Pesce C, Deaglio S, Ciccone E, Malavasi F, Grossi CE: Death of T cell precursors in the human thymus: a role for CD38. Int Immunol. 2003 Sep;15(9):1105-16., URL: http://www.ncbi.nlm.nih.gov/pubmed/12917263,*Brown KN, Trichel A, Barratt-Boyes SM: Parallel loss of myeloid and plasmacytoid dendritic cells from blood and lymphoid tissue in simian AIDS. J Immunol. 2007 Jun 1;178(11):6958-67., URL: http://www.ncbi.nlm.nih.gov/pubmed/term=brown+kn+,*Zimmer MI, Larregina AT, Castillo CM, Capuano S 3rd, Falo LD Jr, Murphey-Corb M, Reinhart TA, Barratt-Boyes SM: Disrupted homeostasis of Langerhans cells and interdigitating dendritic cells in monkeys with AIDS. Blood. 2002 Apr 15;99(8):2859-68., URL: http://www.ncbi.nlm.nih.gov/pubmed/term=zimmer+mi,*Wood GS, Burns BF, Dorfman RF, Warnke RA: The immunohistology of non-T cells in the acquired immunodeficiency syndrome. Am J Pathol. 1985 Sep;120(3):371-9., URL: http://www.ncbi.nlm.nih.gov/pubmed/term=am+j+path

NCBI and Uniprot Product Information

• NCBI GI #: 288558852
• NCBI GeneID: 909
• UniProt Accession #: P06126; Q13962; Q5TDJ8; Q9UMM4; Q9Y5M5; D3DVD7
• Molecular Weight: 37,077 Da
• NCBI Official Full Name: T-cell surface glycoprotein CD1a
• NCBI Official Synonym Full Names: CD1a molecule
• NCBI Official Symbol: CD1A
• NCBI Official Synonym Symbols: R4; T6; CD1; FCB6; HTA1
• NCBI Protein Information: T-cell surface glycoprotein CD1a
• UniProt Protein Name: T-cell surface glycoprotein CD1a
• Protein Family: T-cell surface glycoprotein
• UniProt Gene Name: CD1A
• UniProt Synonym Gene Names: hTa1 thymocyte antigen

NCBI Description

This gene encodes a member of the CD1 family of transmembrane glycoproteins, which are structurally related to the major histocompatibility complex (MHC) proteins and form heterodimers with beta-2-microglobulin. The CD1 proteins mediate the presentation of primarily lipid and glycolipid antigens of self or microbial origin to T cells. The human genome contains five CD1 family genes organized in a cluster on chromosome 1. The CD1 family members are thought to differ in their cellular localization and specificity for particular lipid ligands. The protein encoded by this gene localizes to the plasma membrane and to recycling vesicles of the early endocytic system. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2016]

Uniprot Description

Antigen-presenting protein that binds self and non-self lipid and glycolipid antigens and presents them to T-cell receptors on natural killer T-cells.