Mouse anti-Mouse CD19 Monoclonal Antibody | anti-CD19 antibody

Mouse Anti-Mouse CD19-APC

Cat. Num. AAA673251

• Gene Names: CD19; B4; CVID3; MGC12802
• Reactivity: Mouse
• Applications: Flow Cytometry
• Synonyms: CD19; Monoclonal Antibody; Mouse Anti-Mouse CD19-APC; Mouse Anti-Mouse CD19 - Allophycocyanin; Mouse Anti-Mouse CD19; anti-CD19 antibody

• Host: Mouse
• Reactivity: Mouse
• Clonality: Monoclonal
• Isotype: Mouse (129 x C57BL/6) IgA kappa
• Clone Number: MB19-1
• Specificity: CD19 B cell differentiation antigen, Mr 95 kDa; CD19 is a monomeric transmembrane glycoprotein expressed at relatively constant levels throughout B cell development from early pro-B/pre-B cells (i.e., B220^+/CD43^+/HSA^+) through fully differentiated B cell stages. ^1-7 Terminally differentiated plasma cells do not express CD19. ^1 In humans, the CD19 molecule on the surface of mature B cells associates with CD21 (CR-2) and CD81 (TAPA-1), and this multimolecular complex synergizes with surface immunoglobulin to provide signal transduction and promote cellular activation. ^3, 4 All splenic and peritoneal IgM^+ cells of both B-1 and B-2 lineages are CD19^+, with B-1 cells expressing higher levels of CD19 than B-2 cells in these sites. ^1, 7 Recent studies with CD19-deficient mice have suggested that this molecule may not be required for normal generation and maturation of B cells in the bone marrow. ^7
• Form/Format: Allophycocyanin (APC) Conjugate
• Sequence Length: 556

• Applicable Applications for anti-CD19 antibody: Flow Cytometry
• Application Notes: Flow Cytometry Suggested Dilution: <= 0.3 ug/10^6 cells; Since applications are varies, you should determine the optimum working dilution for the product that is appropriate for your specific need.
• Immunogen: CD19^+ mouse pre-B cell line, 300.19
• Characterization: To ensure lot-to-lot consistency, each batch of monoclonal antibody is tested by flow cytometry to conform to characteristics of a standard reference reagent.
• Preparation and Storage: The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2- 8 degree C. ; The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C. ; The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C. ; The R-phycoerythrin (R-PE) and allophycocyanin (APC) conjugates are supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze! ; The Spectral Red (SPRD), R-phycoerythrin-Cyanine 5.5 (R-PE-CY 5.5), Allophycocyanin-Cyanine 5.5 (APC-CY 5.5), and Allophycocyanin- Cyanine 7 (APC-CY 7) conjugates are supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze! ; Protect conjugated forms from light. Each reagent is stable for the period shown on the bottle label if stored as directed.

References

1. Sato, S., N. Ono, D.A. Steeber, D.S. Pisetsky, and T.F. Tedder. 1996. J. Immunol. 157:4371-4378.
2. Krop, I., A.R. Fougerolles, R.R. Hardy, M. Allison, M.S. Schlissel, and D.T. Fearon. 1996. Eur. J. Immunol. 26:238.
3. Krop, I., A.L. Shaffer, D.T. Fearon, and M.S. Schlissel. 1996. J. Immunol. 157:48.
4. Fearon, D.T. 1993. Curr. Opin. in Immunol. 5:341.
5. Tedder, T.F., L.-J. Zhou, and P. Engel. 1994. Immunol. Today 15:437.
6. Rickert, R.C., K. Rajewsky, and J. Roes. 1995. Nature 376:352.
7. Engel, P., L.-J. Zhou, D.C. Ord, S. Sato, B. Koller, and T.F. Tedder. 1995. Immunity 3:39.
8. Rolink, A., E. ten Boekel, F. Melchers, D.T. Fearon, I. Krop, and J. Andersson. 1996. J. Exp. Med. 183:187.

NCBI and Uniprot Product Information

• NCBI GI #: 901823
• NCBI GeneID: 930
• UniProt Accession #: P15391; Q96S68; Q9BRD6; A0N0P9; Q6ZNU2; Q71UW0
• NCBI Official Full Name: CD19
• NCBI Official Synonym Full Names: CD19 molecule
• NCBI Official Symbol: CD19
• NCBI Official Synonym Symbols: B4; CVID3; MGC12802
• NCBI Protein Information: B-lymphocyte antigen CD19; OTTHUMP00000122551; differentiation antigen CD19; T-cell surface antigen Leu-12; B-lymphocyte surface antigen B4
• UniProt Protein Name: B-lymphocyte antigen CD19
• Protein Family: B-lymphocyte antigen
• UniProt Gene Name: CD19
• UniProt Entry Name: CD19_HUMAN

NCBI Description

Lymphocytes proliferate and differentiate in response to various concentrations of different antigens. The ability of the B cell to respond in a specific, yet sensitive manner to the various antigens is achieved with the use of low-affinity antigen receptors. This gene encodes a cell surface molecule which assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation. [provided by RefSeq]

Uniprot Description

Function: Assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation.

Subunit structure: Forms a complex with CD21, CD81 and CD225 in the membrane of mature B cells. Interacts with VAV. Interacts with GRB2 and SOS when phosphorylated on Tyr-348 and/or Tyr-378. Interacts with PLCG2 when phosphorylated on Tyr-409. Ref.11

Subcellular location: Membrane; Single-pass type I membrane protein.

Post-translational modification: Phosphorylated on serine and threonine upon DNA damage, probably by ATM or ATR. Phosphorylated on tyrosine following B-cell activation. Ref.10 Ref.11 Ref.12

Involvement in disease: Defects in CD19 are the cause of immunodeficiency common variable type 3 (CVID3) [

MIM:613493]; also called antibody deficiency due to CD19 defect. CVID3 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B cells is usually in the normal range, but can be low. Ref.13

Sequence similarities: Contains 2 Ig-like C2-type (immunoglobulin-like) domains.

Sequence caution: The sequence AAA35533.1 differs from that shown. Reason: Frameshift at position 396.