Testing Data
(Surface Staining of human peripheral blood cells with anti-human CD11a:APC )
Mouse anti-Human CD11a Monoclonal Antibody | anti-CD11a antibody
Mouse Anti Human CD11a: Low Endotoxin
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant and 0.2 um filter sterilized.
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant and 0.2 um filter sterilized.
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
This product should be stored undiluted.
Testing Data
(Surface Staining of human peripheral blood cells with anti-human CD11a:APC )
Testing Data
(Surface Staining of human peripheral blood cells with anti-human CD11a:APC )
Testing Data
(Surface Staining of human peripheral blood cells with anti-human CD11a:RPE )
Testing Data
(Surface Staining of human peripheral blood cells with anti-human CD11a:RPE )
Testing Data
(Surface Staining of human peripheral blood cells with anti-human CD11a:FITC )
Testing Data
(Surface Staining of human peripheral blood cells with anti-human CD11a:FITC )
Testing Data
(Figure A. FITC conjugated Mouse anti Human CD4 and RPE conjugated mouse IgG1 isotype control . Figure B. FITC conjugated Mouse anti Human CD4 and RPE conjugated Mouse anti Human CD11a . All experiments were performed on human peripheral blood lymphocytes blocked with 10% human serum, gated on live single cell lymphocytes. Data acquired on the ZE5 Cell Analyzer.)
Testing Data
(Figure A. FITC conjugated Mouse anti Human CD4 and RPE conjugated mouse IgG1 isotype control . Figure B. FITC conjugated Mouse anti Human CD4 and RPE conjugated Mouse anti Human CD11a . All experiments were performed on human peripheral blood lymphocytes blocked with 10% human serum, gated on live single cell lymphocytes. Data acquired on the ZE5 Cell Analyzer.)
LFA-1 is a receptor for ICAMs, JAM-1 and F11R. Its interaction with endothelial ligands, such as ICAM-1 and JAM-1 is responsible for leukocyte arrest and extravasation (Osterman et al.2002). In addition to its role in leukocyte adhesion and transmigration, LFA-1 is involved in T cell differentiation (Verma et al. 2016, Verman and Kelleher 2017) and is required for the effective lysis of infected cells by cytotoxic T cells (Anikeeva et al. 2005, Pontes Ferreira et al. 2017). LFA-1 also contributes to NK cell cytotoxicity (Domingo et al. 2004) and participates to the clearance of apoptotic neutrophils by macrophages (Kristóf et al. 2013). The interaction of LFA-1 with ICAM-1 is the target of Lifitegrast, a drug approved for the treatment of Dry Eye Disease (Abidi et al. 2016).
Mouse anti Human CD11 antibody, clone MEM-25 inhibits the cytotoxic activity of NK cells (Bazil et al. 1990).
NCBI and Uniprot Product Information
NCBI Description
ITGAL encodes the integrin alpha L chain. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form the integrin lymphocyte function-associated antigen-1 (LFA-1), which is expressed on all leukocytes. LFA-1 plays a central role in leukocyte intercellular adhesion through interactions with its ligands, ICAMs 1-3 (intercellular adhesion molecules 1 through 3), and also functions in lymphocyte costimulatory signaling. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
ITGAL: Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. It is involved in a variety of immune phenomena including leukocyte-endothelial cell interaction, cytotoxic T-cell mediated killing, and antibody dependent killing by granulocytes and monocytes. Belongs to the integrin alpha chain family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Motility/polarity/chemotaxis; Membrane protein, integral; Cell adhesion; Cell surface
Chromosomal Location of Human Ortholog: 16p11.2
Cellular Component: cell surface; membrane; plasma membrane; immunological synapse; intercellular junction; integrin complex; external side of plasma membrane
Molecular Function: protein binding; protein heterodimerization activity; metal ion binding; protein complex binding; ICAM-3 receptor activity; cell adhesion molecule binding
Biological Process: integrin-mediated signaling pathway; extracellular matrix organization and biogenesis; regulation of immune response; cell-matrix adhesion; activated T cell proliferation; positive regulation of calcium-mediated signaling; signal transduction; receptor clustering; heterophilic cell adhesion; leukocyte adhesion; T cell activation via T cell receptor contact with antigen bound to MHC molecule on antigen presenting cell; positive regulation of T cell proliferation; inflammatory response; blood coagulation; cell adhesion; cell motility; leukocyte migration
Research Articles on CD11a
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Product Notes
The CD11a itgal (Catalog #AAA225740) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Anti Human CD11a: Low Endotoxin reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD11a can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Functional Assay (FN), Immunoprecipitation (IP). FC/FACS: Min. Dilution: Neat, Max. Dilution: 1/50 Use 10ul of the suggested working dilution to label 1x106 cells in 100ul. Researchers should empirically determine the suitability of the CD11a itgal for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD11a, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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