Mouse anti-Rat CD16a Monoclonal Antibody | anti-FCGR3A antibody
Anti-CD16a Antibody, Mouse Monoclonal
Gene Names
Fcgr3a; Fcgr4
Reactivity
Rat
Applications
ELISA
Purity
Protein A
Synonyms
CD16a; Monoclonal Antibody; Anti-CD16a Antibody; Mouse Monoclonal; CD16a/FCGR3A Antibody; Mouse MAb; Fc fragment of IgG; low affinity IIIa; receptor (CD16a); anti-FCGR3A antibody
Host
Mouse
Reactivity
Rat
Clonality
Monoclonal
Isotype
Mouse IgG1
Clone Number
4B1H6
Specificity
Rat CD16a/FCGR3A.
No cross-reactivity in ELISA with Human cell lysate (293 cell line)
No cross-reactivity in ELISA with Human cell lysate (293 cell line)
Purity/Purification
Protein A
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-FCGR3A antibody
ELISA (EIA)
Application Notes
ELISA: 1:1000-1:2000
Immunogen
Recombinant Rat CD16a/FCGR3A Protein
Conjugation
Unconjugated
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Rat CD16a/FCGR3A (rR CD16a/FCGR3A). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
Related Product Information for anti-FCGR3A antibody
The Fc receptor with low affinity for IgG (FCGR3, or CD16) is encoded by 2 nearly identical genes, FCGR3A and FCGR3B, resulting in tissue-specific expression of alternative membrane-anchored isoforms. FCGR3A, it is also known as CD16a, encodes a transmembrane protein expressed on activated monocytes/macrophages, natural killer (NK) cells, and a subset of T cells. CD16a/FCGR3A is a receptor expressed on NK cells that facilitates antibody dependent cellular cytotoxicity (ADCC) by binding to the Fc portion of various antibodies. CD16a/FCGR3A also has a broader function. CD16a/FCGR3A is directly involved in the lysis of some virus-infected cells and tumor cells by NK cells, independent of antibody binding. Cross-linking of CD16a/FCGR3A on NK cells resulted in increased intracellular Ca2+ levels and a cascade of biochemical events similar to those activated by the T cell receptor. CD16a/FCGR3A on human NK cells is a lysis receptor that mediates the direct killing of some virus infected and tumor cells, independent of antibody ligation.
Product Categories/Family for anti-FCGR3A antibody
References
David Dornan, et al. Effect of FCGR2A and FCGR3A variants on CLL outcome. Blood. 2010 116: 4212-4222
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
Fc fragment of IgG intermediate affinity IV receptor
NCBI Official Synonym Full Names
Fc fragment of IgG receptor IIIa
NCBI Official Symbol
Fcgr3a
NCBI Official Synonym Symbols
Fcgr4
NCBI Protein Information
Fc fragment of IgG receptor IIIa; Fc fragment of IgG, low affinity IIIa, receptor
Protein Family
Similar Products
Product Notes
The FCGR3A (Catalog #AAA8112610) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-CD16a Antibody, Mouse Monoclonal reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's CD16a can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA). ELISA: 1:1000-1:2000. Researchers should empirically determine the suitability of the FCGR3A for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD16a, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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