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Western Blot (WB) (Western blot analysis of CD168 on human CD168 recombinant protein using anti-CD168 antibody at 1/1, 000 dilution.)

Mouse anti-Human CD168 Monoclonal Antibody | anti-CD168 antibody

CD168 Antibody

Gene Names
HMMR; CD168; IHABP; RHAMM
Reactivity
Human
Applications
Western Blot, Flow Cytometry, Functional Assay
Purity
ProA affinity purified
Synonyms
CD168; Monoclonal Antibody; CD168 Antibody; CD168 antibody; CD168 antigen antibody; HMMR antibody; HMMR_HUMAN antibody; Hyaluronan mediated motility receptor antibody; Hyaluronan-mediated motility receptor (RHAMM) antibody; IHABP antibody; Intracellular hyaluronic acid-binding protein antibody; MGC119494 antibody; MGC119495 antibody; OTTHUMP00000196920 antibody; Receptor for hyaluronan-mediated motility antibody; RHAMM antibody; anti-CD168 antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Clone Number
C9-F2
Purity/Purification
ProA affinity purified
Form/Format
1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Applicable Applications for anti-CD168 antibody
Western Blot (WB), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:500-1:2000
FC/FACS: 1:50-1:100
Immunogen
Recombinant protein
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C.

Western Blot (WB)

(Western blot analysis of CD168 on human CD168 recombinant protein using anti-CD168 antibody at 1/1, 000 dilution.)

Western Blot (WB) (Western blot analysis of CD168 on human CD168 recombinant protein using anti-CD168 antibody at 1/1, 000 dilution.)

Western Blot (WB)

(Western blot analysis of CD168 on HEK293 (1) and CD168-hIgGFc transfected HEK293 (2) cell lysate using anti-CD168 antibody at 1/1, 000 dilution.)

Western Blot (WB) (Western blot analysis of CD168 on HEK293 (1) and CD168-hIgGFc transfected HEK293 (2) cell lysate using anti-CD168 antibody at 1/1, 000 dilution.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of Hela cells with CD168 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).)

Flow Cytometry (FC/FACS) (Flow cytometric analysis of Hela cells with CD168 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).)
Related Product Information for anti-CD168 antibody
Hyaluronic acid (HA) is a nonsulfated glycosaminoglycan that regulates cell adhesion and migration. HA effects are mediated through two receptors, CD44 (also designated HCAM) and the receptor of hyaluronic acid mediated motility (RHAMM). RHAMM, also designated intracellular hyaluronic acid binding protein (IHABP) and CD168, is a matrix receptor, which is linked to the plasma membrane by a GPI anchor and regulates cell motility. RHAMM expression is upregulated in malignant lymphoid tissues and is subsequently implicated in tumor progression and metastasis formation, as well as signal transduction. Although still unclear, RHAMM is thought to exist as several isoforms ranging in size. A variant isoform, designated v4, is a protein that when over-expressed, is thought to be the cause of transformation and metastasis formation in fibroblasts
Product Categories/Family for anti-CD168 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
84,100 Da
NCBI Official Full Name
hyaluronan mediated motility receptor isoform a
NCBI Official Synonym Full Names
hyaluronan-mediated motility receptor (RHAMM)
NCBI Official Symbol
HMMR
NCBI Official Synonym Symbols
CD168; IHABP; RHAMM
NCBI Protein Information
hyaluronan mediated motility receptor; receptor for hyaluronan-mediated motility; intracellular hyaluronic acid-binding protein
UniProt Protein Name
Hyaluronan mediated motility receptor
UniProt Gene Name
HMMR
UniProt Synonym Gene Names
IHABP; RHAMM
UniProt Entry Name
HMMR_HUMAN

NCBI Description

The protein encoded by this gene is involved in cell motility. It is expressed in breast tissue and together with other proteins, it forms a complex with BRCA1 and BRCA2, thus is potentially associated with higher risk of breast cancer. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Dec 2008]

Uniprot Description

HMMR: Involved in cell motility. When hyaluronan binds to HMMR, the phosphorylation of a number of proteins, including PTK2/FAK1 occurs. May also be involved in cellular transformation and metastasis formation, and in regulating extracellular- regulated kinase (ERK) activity. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor, misc.

Chromosomal Location of Human Ortholog: 5q34

Cellular Component: cell surface; membrane; cytoplasm; plasma membrane

Molecular Function: hyaluronic acid binding

Biological Process: glycosaminoglycan metabolic process; carbohydrate metabolic process; pathogenesis; hyaluronan metabolic process; hyaluronan catabolic process

Disease: Breast Cancer

Research Articles on CD168

Similar Products

Product Notes

The CD168 hmmr (Catalog #AAA9435262) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The CD168 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD168 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Flow Cytometry (FC/FACS). WB: 1:500-1:2000 FC/FACS: 1:50-1:100. Researchers should empirically determine the suitability of the CD168 hmmr for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD168, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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