Rat CD16 Monoclonal Antibody | anti-CD16 antibody
RAT ANTI MOUSE CD16
Gene Names
Fcgr3; CD16
Applications
Flow Cytometry, Immunohistochemistry
Synonyms
CD16; Monoclonal Antibody; RAT ANTI MOUSE CD16; FcRIII; anti-CD16 antibody
Host
Rat
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
AT154-2
Specificity
Rat anti Mouse CD16 anitbody, clone AT154-2, recognizes CD16, also known as FcRIII. CD16 is a low affiniity activatory receptor which interacts preferentially with mouse IgG1, IgG2a and IgG2b immune complexes and is involved in various effector functions including phagocytosis, NK antibody dependent cellular cytotoxicity and the release of inflammatory mediators.
In mice, CD16 is expressed on macrophages, NK cells, granulocytes, myeloid precursors and subpopulations of T lymphocytes.
In mice, CD16 is expressed on macrophages, NK cells, granulocytes, myeloid precursors and subpopulations of T lymphocytes.
Form/Format
Purified IgG - liquid, TRIS buffered saline
Applicable Applications for anti-CD16 antibody
Flow Cytometry (FC), Immunohistology - Frozen
Application Notes
Flow Cytometry: 1/10*
Immunohistology - Frozen: 1/100 - 1/1000
*Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Where this product has not been tested in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Immunohistology - Frozen: 1/100 - 1/1000
*Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Where this product has not been tested in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Preservative Stabilisers
0.09 Sodium Azide (NaN3)
Concentration
IgG concentration 1.0 mg/ml
Target Species
Mouse
Immunogen
HEK293F cell expressing CD16 and gamma chain
Fusion Partners
Spleen cells from immunised rats were fused with cells of the NS-1 myeloma cell line
Preparation and Storage
Shelf Life: 18 months form date of dispatch.
Store at 4°C or at -20°C if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use.
Store at 4°C or at -20°C if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
Low affinity immunoglobulin gamma Fc region receptor III
NCBI Official Synonym Full Names
Fc receptor, IgG, low affinity III
NCBI Official Symbol
Fcgr3
NCBI Official Synonym Symbols
CD16
NCBI Protein Information
low affinity immunoglobulin gamma Fc region receptor III
UniProt Protein Name
Low affinity immunoglobulin gamma Fc region receptor III
Protein Family
UniProt Gene Name
Fcgr3
UniProt Synonym Gene Names
IgG Fc receptor III; FcRIII
UniProt Entry Name
FCGR3_MOUSE
Uniprot Description
FCGR2A: low affinity receptor for the Fc region of IgGs. Binding to IgG initiates cellular responses against pathogens and soluble antigens. A type I membrane protein found on monocytes, neutrophils and platelets. SHP-1 associates with this protein to modulate signaling events in myeloid cells.
Protein type: Membrane protein, integral; Cell surface; Receptor, misc.
Cellular Component: external side of plasma membrane; integral to membrane; membrane; plasma membrane
Molecular Function: IgG binding; IgG receptor activity
Biological Process: antibody-dependent cellular cytotoxicity; antigen processing and presentation of exogenous peptide antigen via MHC class I; cell surface receptor linked signal transduction; mast cell activation; neutrophil chemotaxis; phagocytosis, engulfment; phagocytosis, recognition; positive regulation of phagocytosis; positive regulation of tumor necrosis factor biosynthetic process; positive regulation of type I hypersensitivity; positive regulation of type IIa hypersensitivity; positive regulation of type III hypersensitivity; regulation of immune response; serotonin secretion
Research Articles on CD16
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Product Notes
The CD16 fcgr3 (Catalog #AAA224798) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's CD16 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC), Immunohistology - Frozen. Flow Cytometry: 1/10* Immunohistology - Frozen: 1/100 - 1/1000 *Use 10ul of the suggested working dilution to label 1x106 cells in 100ul. Where this product has not been tested in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls. Researchers should empirically determine the suitability of the CD16 fcgr3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD16, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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