Rat anti-Mouse CD16/32 (Fc gamma Receptor) Monoclonal Antibody
Rat Anti-Mouse CD16/32-PE
Reactivity
Mouse
Applications
Flow Cytometry
Synonyms
CD16/32 (Fc gamma Receptor); Monoclonal Antibody; Rat Anti-Mouse CD16/32-PE; Rat Anti-Mouse CD16/32 - Phycoerythrin-Labeled; Rat Anti-Mouse CD16/32 (Fc gamma Receptor); anti-CD16/32 (Fc gamma Receptor) antibody
Host
Rat
Reactivity
Mouse
Clonality
Monoclonal
Isotype
Rat IgG2a
Clone Number
93
Specificity
Murine CD16/32 (CD16/Fc gamma II and CD32/Fc gamma III receptors)
The lymphocyte Fc gamma receptors recognize the Fc portion of IgG, presented either as immune complexes or as free antibody. The different classes of receptors are distinct because of varying size, tissue distribution and affinity for IgGs. The Fc type II receptor is expressed on a wide variety of cells including B cells, hematopoietic cells, monocyte/macrophages, neutrophils, platelets, Langerhans cells, eosinophils, basophils, trophoblasts, and endothelial cells of the placenta.^1, 2 The Fc gamma type III receptors are higher affinity than the type II and are expressed on macrophages, NK cells and neutrophils. Both types of receptors can be expressed on the same cell and in varying ratios.^1 The receptors are constitutively expressed, although cytokines and lymphokines can modulate their expression.^4 Besides identifying Fc gamma R^+ cells, monoclonal antibodies to the Fc gamma II/III receptor have been used to block Fc receptor binding of IgG, Fcmediated signal transduction and effector functions, clearance of immune complexes and to attenuate infection by organisms dependent on Fc gamma R for parasitic invasion.^4-6 The monoclonal antibody 93 recognizes a conformational epitope formed by Fc gamma II and Fc gamma III receptors.
The lymphocyte Fc gamma receptors recognize the Fc portion of IgG, presented either as immune complexes or as free antibody. The different classes of receptors are distinct because of varying size, tissue distribution and affinity for IgGs. The Fc type II receptor is expressed on a wide variety of cells including B cells, hematopoietic cells, monocyte/macrophages, neutrophils, platelets, Langerhans cells, eosinophils, basophils, trophoblasts, and endothelial cells of the placenta.^1, 2 The Fc gamma type III receptors are higher affinity than the type II and are expressed on macrophages, NK cells and neutrophils. Both types of receptors can be expressed on the same cell and in varying ratios.^1 The receptors are constitutively expressed, although cytokines and lymphokines can modulate their expression.^4 Besides identifying Fc gamma R^+ cells, monoclonal antibodies to the Fc gamma II/III receptor have been used to block Fc receptor binding of IgG, Fcmediated signal transduction and effector functions, clearance of immune complexes and to attenuate infection by organisms dependent on Fc gamma R for parasitic invasion.^4-6 The monoclonal antibody 93 recognizes a conformational epitope formed by Fc gamma II and Fc gamma III receptors.
Form/Format
R - phycoerythrin (R - PE) Conjugate
Applicable Applications for anti-CD16/32 (Fc gamma Receptor) antibody
Flow Cytometry
Application Notes
Research Application(s): Blocking of Fc gamma receptors
Flow Cytometry Suggested Dilution: <= 0.2 ug/10^6 cells
Flow Cytometry Suggested Dilution: <= 0.2 ug/10^6 cells
Characterization
To insure lot-to-lot consistency, each batch of product is tested by flow cytometry for conformance with characteristics of a standard reference reagent.
Preparation and Storage
The purified (UNLB) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2-8 degree C.
The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL or 0.2 mg in 2.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The allophycocyanin (APC) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The Spectral Red (SPRD), R-phycoerythrin-Cyanine 7 (R-PE-CY 7) and R-phycoerythrin-Cyanine 5.5 (R-PE-CY 5.5) conjugates are supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below -20 degree C.
Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide-free product at -20 degree C immediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored as directed.
The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL or 0.2 mg in 2.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The allophycocyanin (APC) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The Spectral Red (SPRD), R-phycoerythrin-Cyanine 7 (R-PE-CY 7) and R-phycoerythrin-Cyanine 5.5 (R-PE-CY 5.5) conjugates are supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below -20 degree C.
Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide-free product at -20 degree C immediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored as directed.
References
1. Unkeless, J. C., et al. 1995. Semin. Immunol. 7:37.
2. Warmerdam, P.A., et al. 1992. Immunobiol. 185:175.
3. Huizinga, T. W., et al. 1989. J. Immunol. 142:2365.
4. Ho, A. S-Y., et al. 1995. Mol. and Cell. Biol. 15:5043.
5. John Kearney, personal communication.
6. Kurlander, R.J., D. M. Ellison and J. Hall. 1984. J. Immunol. 133:855.
7. Araujo-Jorge, T., et al. 1993. Infection and Immunity 61:4925.
8. Titus, J. A., et al. 1984. J. Immunol. 133:556.
2. Warmerdam, P.A., et al. 1992. Immunobiol. 185:175.
3. Huizinga, T. W., et al. 1989. J. Immunol. 142:2365.
4. Ho, A. S-Y., et al. 1995. Mol. and Cell. Biol. 15:5043.
5. John Kearney, personal communication.
6. Kurlander, R.J., D. M. Ellison and J. Hall. 1984. J. Immunol. 133:855.
7. Araujo-Jorge, T., et al. 1993. Infection and Immunity 61:4925.
8. Titus, J. A., et al. 1984. J. Immunol. 133:556.
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Product Notes
The CD16/32 (Fc gamma Receptor) (Catalog #AAA673331) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The Rat Anti-Mouse CD16/32-PE reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CD16/32 (Fc gamma Receptor) can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry. Research Application(s): Blocking of Fc gamma receptors Flow Cytometry Suggested Dilution: <= 0.2 ug/10^6 cells. Researchers should empirically determine the suitability of the CD16/32 (Fc gamma Receptor) for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD16/32 (Fc gamma Receptor), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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